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Method for platycerium wallichii tissue culture and rapid propagation

A technology of tissue culture rapid propagation and staghorn fern, which is applied in the field of plant tissue culture, can solve the problems of insufficient system of laboratory tissue culture, etc., and achieve the effect of alleviating the lack of seedlings and shortening the growth cycle

Inactive Publication Date: 2015-06-03
杨惠才
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the advantages of long ornamental period and convenient management of staghorn fern, it is widely used as potted foliage plants in the world at present, but my country has just entered the stage of development and utilization, and the research on staghorn fern is in its infancy. Not perfect enough

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] (1) Disinfection of explants: Select the newly grown young leaves of the sporophylls of robust staghorn fern, soak them in 0.1% potassium permanganate solution for 10 minutes, wash them with sterile water for 4 times, soak them in 75% ethanol for 10 seconds, and wash them with sterile water. After washing with water for 3 times, disinfect with 0.1% mercuric solution for 3 minutes, and then wash with sterile water for 5 times before use.

[0020] (2) Induction culture: Use a scalpel to slightly scratch the surface of the sporophyll after the treatment in step (1), and inoculate it face-up in the induction medium for GGB induction. After inoculation, put it in the light for 14 hours a day, the light intensity is 1500lx, and the culture temperature is 25°C. After 20 days of cultivation, brown root hairs grow on most of the explants, and then the base of the root hairs expands and grows tender green GGB. When they grow to a certain size, the young sporophylls begin to turn ...

Embodiment 2

[0025] (1) Disinfection of explants: Select the newly grown young leaves of the sporophylls of robust staghorn fern, soak them in 0.2% potassium permanganate solution for 12 minutes, wash them with sterile water for 5 times, soak them in 78% ethanol for 11 seconds, and wash them with sterile water. After washing with water for 4 times, disinfect with 0.1% mercury solution for 5 minutes, and then wash with sterile water for 5 times before use.

[0026] (2) Induction culture: Use a scalpel to slightly scratch the surface of the sporophyll after the treatment in step (1), and inoculate it face-up in the induction medium for GGB induction. After inoculation, put it in the light for 15 hours a day, the light intensity is 2000lx, and the culture temperature is 26°C. After culturing for 23 days, brown root hairs grow on most of the explants, and then the base of the root hairs expands and grows tender green GGB. When they grow to a certain size, the young sporophylls begin to turn w...

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Abstract

The invention discloses a method for platycerium wallichii tissue culture and rapid propagation and relates to a method for obtaining the platycerium wallichii test-tube plantlet in a short time through a plant tissue culture technology. The in-vitro tissue culture and rapid propagation method of the platycerium wallichii is built by taking the platycerium wallichii tender sporophyl as an explant, disinfecting the explant, and performing GGB induction, proliferating, differentiating and rooting. The growth cycle is shortened, the shortage of the platycerium wallichii commercialized produced seedlings can be relieved and a foundation is laid for innovating the germplasm resources of the pteridophyte in future.

Description

technical field [0001] The invention relates to a method for plant tissue culture in plant biotechnology, in particular to a method for tissue culture and rapid propagation of staghorn fern. Background technique [0002] Ferns originated from the Silurian and Devonian naked ferns of the Paleozoic Era, and there are more than 12,000 species in the world. In its life history, there are two independent living plant bodies, sporophyte and gametophyte. The sporophyte generation and gametophyte generation alternately appear in the life history of ferns, and the sporophyte generation has a greater advantage in the life history of ferns. . Ferns are extremely sensitive to water and heat conditions, and most of them are shade plants and epiphytes, and most groups need forest vegetation to provide their living environment. Due to the diverse types of forest ecosystems in China, there are complete and rich species of ferns. Ferns have high medicinal value. They contain various s...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 杨惠才
Owner 杨惠才
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