Semiliquid in-vitro tissue culture and rapid propagation method for MuSa basjoo

A tissue culture fast propagation, semi-liquid technology, applied in horticultural methods, botanical equipment and methods, horticulture and other directions, can solve the problems of high browning rate of explants, slow bud proliferation, long growth cycle, etc., and shorten the cultivation period. The effect of cycle, seedling character stability, reducing browning rate

Active Publication Date: 2016-04-27
FLOWER RES INST OF YUNNAN ACAD OF AGRI SCI
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AI Technical Summary

Problems solved by technology

[0004] In order to solve the technical problems of high explant browning rate, slow bud proliferation and long growth cycle in the tissue culture and rapid propagation of plantain in existing tissue culture technology, the invention provides a semi-liquid isolated tissue culture and rapid propagation method of plantain, To meet the needs of large-scale production of high-quality plantain seedlings

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0026] (1) Selection and sterilization of explants

[0027] ① In March of each year, choose healthy plantain plants with no pests and diseases to suck buds, rinse them under tap water, peel off the outer layer of old and dead leaves, and soak them in 72% streptomycin sulfate solution for pretreatment for 3 hours , and then peel off the pretreated suction buds into cusps with a diameter of 2 to 3 cm and a length of 3 to 4 cm;

[0028] ②Clean the tooth tips under tap water, soak them in 70% alcohol for 30 minutes under aseptic conditions, then sterilize them with 0.1% mercuric chloride solution for 25 minutes, and rinse them with sterile water for 5-6 minutes. Once again, use sterile filter paper to absorb the surface moisture;

[0029] ③ Cut the cusp of the tooth and leave the growth point and the cut pieces with 4-5 layers of leaf sheaths around it as explants.

[0030] (2) Induction culture

[0031] Inoculate the explants described in step (1) ③ on the semi-liquid inductio...

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Abstract

The invention discloses a semiliquid in-vitro tissue culture and rapid propagation method for MuSa basjoo. According to the method, a semiliquid induction medium comprises an MS culture solution, 6-BA with the concentration of 4 mg/L, NAA with the concentration of 0.15 mg/L, sucrose with the concentration of 30 g/L, activated carbon with the concentration of 0.5 g/L and agar with the concentration of 3 g/L, explants are cultured in a constant-temperature shaking incubator with the shaking frequency of 30 times per minute under the conditions that pH is 5.6-5.8, the temperature is 28-30 DEG C, the illumination intensity is 1,600-2,000 lx and the illumination time of 8 h/d; a multiplication subculture medium mainly comprises the MS culture solution, 6-BA with the concentration of 3 mg/L, NAA with the concentration of 0.15 mg/L and the activated carbon with the concentration of 0.5 g/L; a rooting medium mainly comprises the MS culture solution, NAA with the concentration of 0.5 mg/L, IBA with the concentration of 0.1 mg/L and the activated carbon with the concentration of 0.5 g/L. The browning ratio of the MuSa basjoo is reduced, the induced differentiation ratio and the multiplication coefficient are increased remarkably, and the culture period is shortened.

Description

technical field [0001] The invention belongs to the technical field of tissue culture of Saigon banana, and in particular relates to a semi-liquid rapid in vitro propagation method of Saigon banana. Background technique [0002] Musabasjoo, also known as Musaabb, is one of the edible bananas. Saigon banana has beautiful fruit shape, thin skin and bright yellow color, tender flesh, suitable sweetness, good commerciality, and strong adaptability to barrenness, drought and cold. It is very popular in the market for its resistance to banana leaf spot, bunchy top, mosaic core rot and annual fruiting. It has been widely planted in Guangxi, Guangdong, Yunnan and other places, and has achieved good economic and social benefits, which has increased the enthusiasm of banana farmers to plant Saigon bananas. Traditional seedling propagation is difficult to meet the demand for banana seedlings in the market. With the market With the development of the economy, the supply of its product...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 苏艳杨宝明瞿素萍张艺萍王继华王丽花杨秀梅张丽芳许凤
Owner FLOWER RES INST OF YUNNAN ACAD OF AGRI SCI
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