Tissue culture of Ypsilandra thibetica and planting method

Abstract

The invention provides a method for in vitro tissue culture and nuisance-free planting by taking maternal tissue leaves of Ypsilandra thibetica as explant materials. Large quantities of tissue culture seedlings can be obtained by the method, which meets the planting requirement. In the method, plantation in a natural forest is adopted to overcome pollution caused by pesticides and heavy metals, the survival rate reaches 95.5%, new medicinal materials can be supplied within 3 years, weight of an individual plant is twice more than that of wild medicinal materials and non-proliferated medicinal materials at the same year, the content of effective ingredients which can induce uterus contraction and arrest bleeding is higher than that of the wild medicinal materials at the same year, thus meeting the requirement of pharmaceutical enterprises for raw medicinal materials. The method helps culture the tissue culture seedlings in batches and plant the tissue culture seedlings at a large area, and obtain the nuisance-free medicinal materials 2 years earlier, therefore, the method has popularization value.

Description

Technical field: [0001] The invention belongs to the technical field of plant tissue culture and transplanting planting, and in particular relates to a tissue culture technology and a pollution-free planting method of a new medicinal material of the Liliaceae plant, Arthia chinensis. Background technique: [0002] Yarui flower plant is called Shifengdan and Suibeidan among the people. It has the functions of clearing away heat, detoxifying, dispelling stagnation, diuresis, and mainly treating tuberculosis and edema. There are four kinds of plants in this genus, namely Y. Flower (Y.yunnanensis var.micranthaHand-Mazz); Alpina wang et Tang; Xiaoya stamen (Y.cavaleriei Levl et Vant), all belong to traditional Chinese medicines that have not been listed in the domestic market, and have been The Yunnan Provincial Food and Drug Administration has included it in the standard of traditional Chinese medicinal materials in Yunnan Province, and the 2005 edition of Yi Nationality Medicin...

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Problems solved by technology

[0005] Aiming at the above-mentioned deficiencies of the prior art, the present invention provides a kind of arastina tissue cultivation and nuisance-free planting technology. By adopting the method of the present invention, a large number of tissue-cultured seedlings can be obtained to meet the planting needs; and planting in natural forest areas can overcome pesticide and heavy metal pollution. , the survival rate is 95.5%, and new medicinal materials can be provided within three years. The weight of a single plant is 2 times higher than that of wild and non-propagative planted medicinal materials in the same year, and the content of active ingredients for uterine hemostasis is higher than that of wild medicinal materials in the same year; and it meets the requirements of pharmaceutical companies for original medicinal materials. needs, it can be cultivated in batches and planted in large areas, and the pollution-free medicinal materials can be obtained two years earlier, which has practical value

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