Method for culturing wild jujube grafted by jujube in-vitro tissue culture seedling

Abstract

The invention relates to a method for culturing a wild jujube grafted by a jujube in-vitro tissue culture seedling, which is characterized by comprising the following steps of: (1) carrying out bud forcing treatment on a wild jujube seed, and seeding the wild jujube seed in a nutrition bowl filled with medium when the seed begins to bud; (2) hardening a tissue culture seedling growing to the length of 5-7cm and being cultured for 25-35 days in an opened bottle under hard light for 5-10 days, shearing the tissue culture seedling into a stem with two buds, wherein the stem with the two buds is used as a scion; and cutting the lower part of the scion to be wedged; (3) after the main leaf of a wild jujube gives off, cutting off a growing point, remaining two seed leaves, cutting from the middle of the two seed leaves by using a blade through a cleft graft method, meanwhile, rapidly inserting the wedged part of the scion into a cut, and aligning the scion to two ends of a stock stem and twining by the sealing film; and (4) cutting off sprout tillers of the wild jujube in time after one week since grafting, and growing for 15-30 days to obtain a grafted seedling. The method has the beneficial effects that: 1, the problem that the jujube tissue culture seedling is difficult or unstable in rooting can be solved; and 2, the grafted seedling is directly obtained without being limited by seasons and can be produced all the year round.

Description

technical field [0001] The invention relates to a method for growing jujube seedlings by grafting jujube seedlings outside the test tube. Background technique [0002] The most commonly used seedling propagation method of jujube tree at present is root tiller seedling division seedling raising method and wild jujube grafting propagation technique. The quantity and quality of root tiller seedlings are affected by the age of the mother plant, the meristematic ability of the stem root system, the induction method of trenching treatment, and the season. The reproductive coefficient is low and the quality of the seedlings is uneven; Strains, the purity of seedling varieties or strains cannot be guaranteed, and the production quantity of scions is limited. [0003] The use of tissue culture technology greatly improves the breeding speed of jujube seedlings. According to theoretical calculations, hundreds of thousands of tissue culture seedlings can be obtained within one year, an...

AI Technical Summary

Problems solved by technology

[0004] The micro-grafting technology of jujube tree tissue culture seedlings in test tubes needs to be operated under sterile conditions, and there are problems such as high operational technical requirements, strict cultivation conditions, and cumbersome technical links.