Rabbit hemorrhagic disease virus mutant strain, construction method and application thereof
A technology of rabbit viral hemorrhage and its construction method, which is applied in the field of rabbit viral hemorrhagic virus mutant strains and its construction, can solve problems such as production difficulties, residual live virus, and increased production costs, and achieve good immune protection effect
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[0052] The present invention will be described in detail below in conjunction with specific embodiments. The following examples will help those skilled in the art to further understand the present invention, but do not limit the present invention in any form. It should be pointed out that for those of ordinary skill in the art, a number of modifications and improvements can be made without departing from the concept of the present invention. These all belong to the protection scope of the present invention.
[0053] 1 Materials and methods
[0054] 1.1 Plasmids, cells and viruses
[0055] The plasmid pTVT-RHDV has been published in the following documents: Yang Zongwei, Zhang Weiwei, Chen Zongyan, Li Chuanfeng, Wu Run, Liu Guangqing. Optimization of the infectious clone of rabbit hemorrhagic disease virus[J].Journal of Gansu Agricultural University,2011,03:8-12;
[0056] BHK21 cells (BSR cells) expressing T7 polymerase are passaged and preserved in our laboratory, and the medium is ...
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