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Rabbit hemorrhagic disease virus mutant strain, construction method and application thereof

A technology of rabbit viral hemorrhage and its construction method, which is applied in the field of rabbit viral hemorrhagic virus mutant strains and its construction, can solve problems such as production difficulties, residual live virus, and increased production costs, and achieve good immune protection effect

Active Publication Date: 2015-12-23
SHANGHAI VETERINARY RES INST CHINESE ACAD OF AGRI SCI
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  • Abstract
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AI Technical Summary

Problems solved by technology

For example, virus escape may occur during the production process, live virus may remain during the chemical inactivation process, and the inoculation dose is high and side effects are large, etc.
In addition, with the sharp reduction of non-immunized rabbits, the production cost is also increasing, and it has brought great difficulties to the production of the vaccine.

Method used

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  • Rabbit hemorrhagic disease virus mutant strain, construction method and application thereof
  • Rabbit hemorrhagic disease virus mutant strain, construction method and application thereof
  • Rabbit hemorrhagic disease virus mutant strain, construction method and application thereof

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Embodiment Construction

[0052] The present invention will be described in detail below in conjunction with specific embodiments. The following examples will help those skilled in the art to further understand the present invention, but do not limit the present invention in any form. It should be noted that those skilled in the art can make several modifications and improvements without departing from the concept of the present invention. These all belong to the protection scope of the present invention.

[0053] 1 Materials and methods

[0054] 1.1 Plasmids, Cells and Viruses

[0055] Plasmid pTVT-RHDV has been disclosed in the following literature: Yang Zongwei, Zhang Weiwei, Chen Zongyan, Li Chuanfeng, Wu Run, Liu Guangqing. Optimization of infectious clone of rabbit hemorrhagic disease virus[J]. Journal of Gansu Agricultural University, 2011,03:8-12;

[0056] BHK21 cells (BSR cells) expressing T7 polymerase were subcultured and preserved in our laboratory, and the culture medium was DMEM (Hyclo...

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Abstract

The invention discloses a rabbit hemorrhagic disease virus mutant strain, a construction method and application thereof. The rabbit hemorrhagic disease virus mutant strain contains a genome coded capsid protein VP60, a secondary structure protein VP10 and non-structural protein p11, p28, p35, p32, VPg, 3C like protease and RNA dependent RNA polymerase. Specifically, the capsid protein contains RGD short peptide, which is composed of the 305th arginine, 306th glycine and 307th aspartic acid of capsid protein VP60. The rabbit hemorrhagic disease virus mutant strain constructed by the invention can achieve stable proliferation and stable passage in RK13 cells, and can reach a good protective effect on hosts after cytotoxicity inactivation. The construction method can promote batch production of vaccines and reduce the vaccine production cost.

Description

technical field [0001] The invention belongs to the field of recombinant viruses, and in particular relates to a mutant strain of rabbit viral hemorrhagic disease virus and its construction method and application. Background technique [0002] Rabbit Hemorrhagic Disease Virus (RHDV) is a virulent virus that can cause acute and fatal infectious diseases in a variety of rabbits and hares, and its fatality rate is as high as 90%-100%. Rabbit disease caused by RHDV infection, also known as "rabbit plague", was first discovered in Jiangsu Province, China in 1984. Subsequently, many countries in Asia, Europe, Africa, and South America also successively reported the outbreak of the disease. Today, the epidemic is still widespread, posing a serious threat to the development of the rabbit industry, and is regarded as the "number one killer" of the rabbit industry. [0003] RHDV belongs to Caliciviridae, Lagovirus, and European Brown Hare Syndrome (EBHSV). All the RHDVs discovered ...

Claims

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Application Information

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IPC IPC(8): C12N7/00C12N15/85C12N15/40A61K39/12A61P31/14C12R1/93
Inventor 刘光清朱杰孟春春李传峰陈宗艳
Owner SHANGHAI VETERINARY RES INST CHINESE ACAD OF AGRI SCI
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