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A sticky red yeast and feed technology, applied in the field of bioengineering, can solve the problems of high production cost, high toxicity, and difficulty in mass production
Inactive Publication Date: 2010-12-15
黑龙江大荒春酒业有限公司
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Problems solved by technology
[0003] Chemical synthesis of β-carotene has high toxicity, low biological activity, complex process and high cost; β-carotene extracted from natural products has extremely high biological activity, but it is difficult to obtain due to various factors such as geographical location, raw materials and yield. It is produced in large quantities, and the production cost is too high, so it is difficult to be accepted by the general market; the microbial fermentation method is not affected by environmental conditions and other conditions, and has a high yield, so it is the best method to produce β-carotene
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Embodiment 1
[0034] 1. Selection of Rhodotorula viscosus and preparation of medium
[0035] The Rhodotorula glutinis selected in the present invention was purchased from China Industrial Microorganism Strain Collection Center, numbered 31596, and the strain culture medium was: 5°Bé wort.
[0036] 2. Preparation of fermentation strains
[0037] 2.1 Preparation of primary fermentation strains
[0038] Measure 100ml of 5°Bé wort into a 500ml Erlenmeyer flask, sterilize at 121°C for 20 minutes and cool to 28-30°C, use an inoculation loop to inoculate one ring of the slant of Rhodotorula viscosus strain, at 28-30°C, 120 rpm Minute shaker culture and culture for 36 hours, as a primary fermentation strain.
[0039] 2.2 Preparation of secondary fermentation strains
[0040] 5°Bé wort, sterilized at 121°C for 20 minutes, cooled to 28-30°C, inoculated with primary fermentation strains at 10% by weight, and cultured on a shaking table at 28-30°C, 120 rpm for 48 hours.
[0041] Feeding conditions ...
Embodiment 2
[0060] 1. Selection of Rhodotorula viscosus and preparation of medium
[0061] The Rhodotorula glutinis selected in the present invention was purchased from China Industrial Microorganism Strain Collection Center, numbered 31596, and the strain culture medium was: 5°Bé wort.
[0062] 2. Preparation of fermentation strains
[0063] 2.1 Preparation of primary fermentation strains
[0064] Measure 100ml of 5°Bé wort into a 500ml Erlenmeyer flask, sterilize at 121°C for 20 minutes and cool to 28-30°C, use an inoculation loop to inoculate one ring of the slant of Rhodotorula viscosus strain, at 28-30°C, 120 rpm Minute shaker culture and culture for 36 hours, as a primary fermentation strain.
[0065] 2.2 Preparation of secondary fermentation strains
[0066] 5°Bé wort, sterilized at 121°C for 20 minutes, cooled to 28-30°C, inoculated with primary fermentation strains at 10% by weight, and cultured on a shaking table at 28-30°C, 120 rpm for 48 hours.
[0067]Feeding conditions o...
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Abstract
The invention provides a method for preparing a rhodotorula glutinis feed. The method comprises the following steps of: preparing a primary fermentative strain of 5 degree Be wort by using the rhodotorula glutinis as a parent strain; inoculating the primary fermentative strain into the 5-degree Be wort in a weight ratio of 10 percent; after 12 hours, continuously feeding a nutrient source containing 2 percent of maltose, 0.2 percent of urea and 0.1 percent of ammonium sulfate in 16 hours; culturing for 48 hours, wherein the viable count of the rhodotorula glutinis in the fermentation broth is more than or equal to 109 per milliliter, and the rhodotorula glutinis is used as a secondary fermentative strain; inoculating the secondary fermentative strain accounting for 5 to 8 percent of a distiller grain fermentation medium; tiling the uniformly stirred fermentation medium in a fermenting tank to ferment at the temperature of between 28 and 30 DEG C for 60 hours, wherein the thickness of the medium is between 20 and 25cm; and drying the strain by hot wind through a feed drier at the temperature of between 50 and 60 DEG C until the moisture of the strain is lower than 5 percent, and packaging the strain to obtain the rhodotorula glutinis feed finished product.
Description
(1) Technical field [0001] The invention relates to a method for preparing a rhodotorula feed, in particular to a method for producing a rhodotorula feed by fermenting distiller's grains with the rhodotorula, which belongs to the field of bioengineering. (2) Background technology [0002] Rhodotorula viscosus can produce a large amount of β-carotene during the metabolic process, β-carotene is a kind of carotenoid, used as a feed additive, can effectively improve the nutritional status of animals, and can also improve the quality of egg yolk, poultry and meat. The color of the skin. Carotenoids can enhance the reproductive capacity of animals and enhance immune function. Natural carotenoid is a rare drug with extensive immune function and an indispensable green feed additive, which has been widely used in animal husbandry, poultry, and aquaculture. At present, in most areas of our country, it is necessary to add appropriate amount of pigment products to poultry feed to meet...
Claims
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Application Information
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