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Method for culturing synthetic bacteria in rapid growth and acid production and obtained synthetic bacteria

A technology of complex flora and culture methods, applied in the direction of microorganism-based methods, biochemical equipment and methods, restoration of contaminated soil, etc., can solve the problems of difficult cultivation of microorganisms, achieve pollution avoidance, shorten growth time, and solve growth problems. slow effect

Active Publication Date: 2017-09-05
CENT SOUTH UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to provide a method for cultivating rapid growth and acid-producing complex flora, to solve the problem of difficult cultivation of microorganisms required for rapid leaching treatment of heavy metal-contaminated soil, and to quickly cultivate acid-producing mixed flora for heavy metals Rapid leaching treatment of contaminated soil provides technical guidance

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] The recessive acidophilus ATCC33463 (Acidiphiliumcryptum), Candida rugosa CCTCC AY93026 (Candida rugosa), nitrogen-fixing acetic acid bacteria CGMCC No.5455 (Acetobacterdiazotrophicus), Rhodotorula glutinis ATCC96365 (Rhodotorulaglutinis) and Pseudomonas aeruginosa CCTCC AB 2013184 (Pseudomonasaeruginosa) artificial co-culture system composed of 15%, 20%, 25%, 30%, 10% ratio: (NH 4 ) 2 SO 4 0.3g / L, MgSO 4 0.05g / L,KCl 0.01g / L,K 2 HPO 4 0.05g / L, Ca(NO 3 ) 2 0.001g / L, add 0.1g / L pyrrhotite particles and 0.5g / L sulfur powder, 1g / L glucose, 0.5g / L yeast powder at the same time, crush pyrrhotite and sublimated sulfur to more than 200 mesh Granules, the microbial concentration after inoculation was 5.0×10 6 per milliliter, the pH value is 3.24, the culture temperature is about 30°C, the stirring and bottom aeration are carried out at the same time, and the aeration rate is 0.20m 3 / min, the stirring speed was 400rpm, after 48 hours of cultivation, the pH dropped to...

Embodiment 2

[0025] The recessive acidophilus ATCC33463 (Acidiphiliumcryptum), Candida rugosa CCTCC AY93026 (Candida rugosa), nitrogen-fixing acetic acid bacteria CGMCC No.5455 (Acetobacterdiazotrophicus), Rhodotorula glutinis ATCC96365 (Rhodotorulaglutinis) and Pseudomonas aeruginosa CCTCC AB 2013184 (Pseudomonasaeruginosa) artificial co-culture system composed of 15%, 20%, 25%, 30%, 10% ratio: (NH 4 ) 2 SO 4 0.3g / L, MgSO 4 0.05g / L,KCl 0.01g / L,K 2 HPO 4 0.05g / L, Ca(NO 3 ) 2 0.001g / L, add 0.1g / L pyrrhotite particles and 0.5g / L sulfur powder, 1g / L glucose, 0.5g / L yeast powder at the same time, crush pyrrhotite and sublimated sulfur to more than 200 mesh Granules, the microbial concentration after inoculation was 8.0×10 6 per milliliter, the pH value is 3.02, the culture temperature is 26°C, stirring and bottom aeration are carried out at the same time, and the aeration rate is 0.20m 3 / min, the stirring speed is 400rpm, cultivated for 56 hours, the pH drops to 2.04, and the bact...

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PUM

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Abstract

The invention discloses a method for culturing synthetic bacteria in rapid growth and acid production and obtained synthetic bacteria. Original strains mainly comprise Acidiphilium cryptum, Candida rugose, Acetobacter diazotrophicus, Rhodotorula glutinis and Pseudomonas aeruginosa. Pyrrhotite powder is added into a culture medium, strain inoculation quantity ranges from 5.0*10<6> / liter to 8.0*10<6> / liter, and aeration culture is performed. By the method, efficient acid production mixed bacteria can be rapidly and substantially cultured, and the pH (potential of hydrogen) of solution is reduced to be around 2.0. The bacteria are applied to rapid leaching treatment for heavy metal contaminated soil and wide in prospect.

Description

technical field [0001] The invention belongs to the technical field of microbial environment treatment, and in particular relates to a compound flora capable of rapid growth and acid production and a cultivation method thereof. The compound flora cultivated by the method is used for rapid leaching treatment of heavy metal-contaminated soil, and the effect is remarkable. Background technique [0002] Heavy metals are difficult to degrade in the soil environment, easy to accumulate in animals and plants, gradually enriched through the food chain, and the concentration can sometimes increase by a million times, and finally enter the human body and cause harm to human health. They are one of the most harmful pollutants to human beings. Soil heavy metal pollution has the characteristics of concealment, long-term, irreversible, etc., and it is extremely difficult to control. [0003] At present, the methods widely used in the treatment of soil heavy metal pollution at home and abr...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/16B09C1/10C12R1/01C12R1/72C12R1/02C12R1/385
CPCB09C1/10B09C1/105C12N1/16C12N1/20
Inventor 刘学端柏连阳刘宏伟蒋慧丹尹华群徐梁枫
Owner CENT SOUTH UNIV
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