Method for culturing synthetic bacteria in rapid growth and acid production and obtained synthetic bacteria
A technology of complex flora and culture methods, applied in the direction of microorganism-based methods, biochemical equipment and methods, restoration of contaminated soil, etc., can solve the problems of difficult cultivation of microorganisms, achieve pollution avoidance, shorten growth time, and solve growth problems. slow effect
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Embodiment 1
[0022] The recessive acidophilus ATCC33463 (Acidiphiliumcryptum), Candida rugosa CCTCC AY93026 (Candida rugosa), nitrogen-fixing acetic acid bacteria CGMCC No.5455 (Acetobacterdiazotrophicus), Rhodotorula glutinis ATCC96365 (Rhodotorulaglutinis) and Pseudomonas aeruginosa CCTCC AB 2013184 (Pseudomonasaeruginosa) artificial co-culture system composed of 15%, 20%, 25%, 30%, 10% ratio: (NH 4 ) 2 SO 4 0.3g / L, MgSO 4 0.05g / L,KCl 0.01g / L,K 2 HPO 4 0.05g / L, Ca(NO 3 ) 2 0.001g / L, add 0.1g / L pyrrhotite particles and 0.5g / L sulfur powder, 1g / L glucose, 0.5g / L yeast powder at the same time, crush pyrrhotite and sublimated sulfur to more than 200 mesh Granules, the microbial concentration after inoculation was 5.0×10 6 per milliliter, the pH value is 3.24, the culture temperature is about 30°C, the stirring and bottom aeration are carried out at the same time, and the aeration rate is 0.20m 3 / min, the stirring speed was 400rpm, after 48 hours of cultivation, the pH dropped to...
Embodiment 2
[0025] The recessive acidophilus ATCC33463 (Acidiphiliumcryptum), Candida rugosa CCTCC AY93026 (Candida rugosa), nitrogen-fixing acetic acid bacteria CGMCC No.5455 (Acetobacterdiazotrophicus), Rhodotorula glutinis ATCC96365 (Rhodotorulaglutinis) and Pseudomonas aeruginosa CCTCC AB 2013184 (Pseudomonasaeruginosa) artificial co-culture system composed of 15%, 20%, 25%, 30%, 10% ratio: (NH 4 ) 2 SO 4 0.3g / L, MgSO 4 0.05g / L,KCl 0.01g / L,K 2 HPO 4 0.05g / L, Ca(NO 3 ) 2 0.001g / L, add 0.1g / L pyrrhotite particles and 0.5g / L sulfur powder, 1g / L glucose, 0.5g / L yeast powder at the same time, crush pyrrhotite and sublimated sulfur to more than 200 mesh Granules, the microbial concentration after inoculation was 8.0×10 6 per milliliter, the pH value is 3.02, the culture temperature is 26°C, stirring and bottom aeration are carried out at the same time, and the aeration rate is 0.20m 3 / min, the stirring speed is 400rpm, cultivated for 56 hours, the pH drops to 2.04, and the bact...
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