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Microbial oil preparation method with biodiesel byproducts as carbon sources

A microbial oil and biodiesel technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of expensive raw materials of biodiesel and expensive cost of microbial oil, achieve short preparation cycle, reduce preparation cost, The effect of large application prospects

Active Publication Date: 2014-12-17
UNIV OF SCI & TECH BEIJING
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The technical problem to be solved by the present invention is to provide a method for preparing microbial oil using crude glycerin, a by-product of biodiesel, as a carbon source. The problem of utilization and disposal of biodiesel, and at the same time solve the problem of expensive raw materials for the preparation of biodiesel

Method used

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  • Microbial oil preparation method with biodiesel byproducts as carbon sources

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Experimental program
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Effect test

Embodiment 1

[0032] The above inoculum was added to the sterilized medium at a ratio of 10% and cultivated for 7 days, the pH of the medium was 6.0, the sterilization temperature was 121°C, the sterilization time was 30 minutes, the shaker speed was 200r / min, crude glycerol Ingredients: 27% glycerin, 18% methanol, 10% ethanol, 45% water. Medium composition: crude glycerol 27g / L (to ensure that the carbon content in the medium is consistent with 20g / L glucose), NH 4 SO 4 5g / L, KH 2 PO 4 1g / L, yeast extract powder 0.5g / L, Mg 2 SO 4 0.5g / L. The rest of the experimental conditions and steps are the same as in Comparative Example 1. At this time, the dry weight of the cells was measured to be 20.5 g / L, and the oil content in the dry weight of the cells was 47.8%.

[0033] From Comparative Example 1, Comparative Example 2 and Example 1, it can be seen that the dry cell weight and oil yield of microbial oils prepared with crude glycerol as carbon source are higher than those of cells when ...

Embodiment 2

[0035] The inoculum was added to the sterilized medium at a ratio of 5% and cultivated for 6 days, the pH was 5.8, the sterilization temperature was 121°C, the sterilization time was 30 minutes, the shaker speed was 180r / min, and the crude glycerin composition: glycerol 25%, methanol 25%, ethanol 15%, water 30%, fatty acid sodium 2%, fatty acid ester 1%, NaCl 2%. Crude glycerol medium composition: crude glycerol 100g / L, NH 4 SO 4 5g / L, KH 2 PO 4 1g / L, yeast extract powder 0.5g / L, Mg 2 SO 4 0.5g / L. Put 32489 5ml of Rhodotorula viscosus obtained after fermentation in a centrifuge, centrifuge at 4000r / min for 30 minutes, take the lower layer of bacteria and put them in an oven, and dry them at 65°C until they reach a constant weight. At this time, the dry weight of the cells was measured to be 16.5 g / L. Put the dried Rhodotorula viscosus 32489 into a centrifuge tube, add 10ml of 4mol / L hydrochloric acid to the dried bacteria, place the shaker to mix well, leave it at room ...

Embodiment 3

[0037] Add the inoculum to the sterilized medium at a ratio of 10% and cultivate for 10 days, the pH is 6.0, the sterilization temperature is 121°C, the sterilization time is 30 minutes, the shaker speed is 220r / min, and the crude glycerin composition: glycerol 45%, methanol 15%, ethanol 5%, water 31%, fatty acid sodium 1%, fatty acid ester 2%, NaCl 1%. Crude glycerol medium composition: crude glycerol 20g / L, NH 4 SO 4 5g / L, KH 2 PO 4 1g / L, yeast extract powder 0.5g / L, Mg 2 SO 4 0.5g / L. Take 32489 5ml of Rhodotorula viscosus obtained after fermentation and put it in a centrifuge, centrifuge at 4000r / min for 30 minutes, take the lower layer of bacteria and put them in an oven, and dry them at 45°C until they reach a constant weight. At this time, the dry weight of the cells was measured to be 13.5 g / L. Put the dried Rhodotorula viscosus 32489 into a centrifuge tube, add 10ml of 4mol / L hydrochloric acid to the dried cells, place the shaker to mix well, leave it at room te...

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Abstract

The invention provides a microbial oil preparation method with biodiesel byproducts as carbon sources and belongs to the technical field of microbial oil preparation. According to the microbial oil preparation method, microbial oil is prepared with the biodiesel byproducts serving as the carbon sources and rhodotorula glutinis 32489 serving as the oil-producing microorganisms. Therefore, the problem of high cost when glucose or pure glycerine (AR) is used as the carbon sources to prepare microbial oil is solved, and crude glycerine is effectively used; the components of the produced microbial oil are almost as same as those of vegetable oil. Compared with vegetable oil, microbial oil has the advantages of being short in the preparation cycle and free of occupying arable land, is a cheap non-crop biomass raw material to prepare biodiesel, can reduce the preparation cost of biodiesel, and has a wide application prospect.

Description

[0001] technical field [0002] The invention relates to the technical field of microbial oil preparation, in particular to a method for preparing microbial oil using crude glycerol, a by-product of biodiesel, as a carbon source. [0003] Background technique [0004] In recent years, it has become a big problem that the total amount of fats and oils required by human beings is only provided by vegetable fats and animal fats. It is becoming more and more important to find other raw materials of fats and oils to produce the fats and oils that people need. In addition, with the increasing price of oil and the depletion of non-renewable resource oil, it becomes an inevitable trend to use other renewable resources to produce energy. Microbial oil is a high-quality raw material for biodiesel production. Microbial oil also contains a variety of unsaturated fatty acids that are beneficial to human health. Therefore, it has important development and utilization values ​​in improvin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/64C12R1/645
CPCY02E50/10
Inventor 汪群慧马英群王娟张琦杨雅娟高振
Owner UNIV OF SCI & TECH BEIJING
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