Chlorella pyrenoidosa, biological environment restoration liquid prepared therefrom and application of chlorella pyrenoidos
A technology of chlorella pyrenoidosa and the environment, applied in the direction of biochemical equipment and methods, applications, microorganisms, etc., can solve the problems of less active algae cell biological fertilizer, reduced content of beneficial microorganisms, obstacles to continuous cropping of crops, etc., to improve the supply situation , increase the content of organic matter, reduce the effect of negative impact
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Embodiment 1
[0047] The chlorella pyrenoidosa of the present embodiment prepares the biological environment restoration liquid, is carried out according to the following steps:
[0048] After culturing Chlorella pyrenoidosa with nutrient solution to the exponential growth phase, mix it evenly with an alginate solution with a mass percent content of 2% to obtain a mixed solution of Chlorella pyrenoidosa; then add it dropwise to a concentration of 0.2 Keep in mol / L calcium chloride solution for 30 minutes to form small balls; then prepare by adding 200 small balls per 1L of the used solution to obtain the biological environment restoration solution;
[0049] The mass volume ratio of the chlorella pyrenoidosa and 2% alginate solution is 1g:50mL, and the volume ratio of the chlorella pyrenoidosa solution to the calcium chloride solution is 1:5-7; The use liquid is a nutrient solution;
[0050] Described nutrient solution contains 0.2g (NH 4 ) 2 SO 4 , 25mg of potassium chloride, 30mg of su...
Embodiment 2
[0054] Here is the functional verification:
[0055] The bio-environment restoration solution prepared in Example 1 is carried out to verify the growth state of rice:
[0056] In the rice seedling stage, there are 2 experimental fields, each of which is 2 mu. One piece that only applies chemical fertilizer is the control group (only applies chemical fertilizer 70kg), and one piece adds the green algae active cell biological fertilizer of the control group 2 / 3 chemical fertilizer and 2L embodiment (applying into chemical fertilizer 46kg and 2L green algae active cell biological fertilizer) is small In the coccus group, observe and record the growth of rice after 20 days and record.
[0057]
[0058] Prepare 2 test fields, each with 3 mu. One piece with only chemical fertilizer applied is the control group (only 60kg of chemical fertilizer is applied), and one piece with 2 / 3 of the chemical fertilizer and 3L of green algae active cell biological fertilizer (applied with 40k...
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