Chlorella pyrenoidosa, biological environment restoration liquid prepared therefrom and application of chlorella pyrenoidos

A technology of chlorella pyrenoidosa and the environment, applied in the direction of biochemical equipment and methods, applications, microorganisms, etc., can solve the problems of less active algae cell biological fertilizer, reduced content of beneficial microorganisms, obstacles to continuous cropping of crops, etc., to improve the supply situation , increase the content of organic matter, reduce the effect of negative impact

Active Publication Date: 2021-03-23
范秀娟
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The root exudates and decomposing products of crops (such as p-hydroxybenzoic acid PA) can inhibit the growth and activity of the roots of continuous cropping plants, and have the strongest self-toxicity, resulting in a decrease in the content of beneficial microorganisms in the soil and an imbalance of microorganisms in the soil. Consecutive cropping obstacles in the growth process
[0006] In order to effectively ensure the potential fertility of the soil, it is necessary to artificially add a large number of beneficial microorganisms to the soil, so as to better ensure the activity of microorganisms in the soil and achieve a comprehensive improvement of soil nutritional conditions. However, research reports on the use of algae active cell biofertilizers less

Method used

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  • Chlorella pyrenoidosa, biological environment restoration liquid prepared therefrom and application of chlorella pyrenoidos
  • Chlorella pyrenoidosa, biological environment restoration liquid prepared therefrom and application of chlorella pyrenoidos
  • Chlorella pyrenoidosa, biological environment restoration liquid prepared therefrom and application of chlorella pyrenoidos

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] The chlorella pyrenoidosa of the present embodiment prepares the biological environment restoration liquid, is carried out according to the following steps:

[0048] After culturing Chlorella pyrenoidosa with nutrient solution to the exponential growth phase, mix it evenly with an alginate solution with a mass percent content of 2% to obtain a mixed solution of Chlorella pyrenoidosa; then add it dropwise to a concentration of 0.2 Keep in mol / L calcium chloride solution for 30 minutes to form small balls; then prepare by adding 200 small balls per 1L of the used solution to obtain the biological environment restoration solution;

[0049] The mass volume ratio of the chlorella pyrenoidosa and 2% alginate solution is 1g:50mL, and the volume ratio of the chlorella pyrenoidosa solution to the calcium chloride solution is 1:5-7; The use liquid is a nutrient solution;

[0050] Described nutrient solution contains 0.2g (NH 4 ) 2 SO 4 , 25mg of potassium chloride, 30mg of su...

Embodiment 2

[0054] Here is the functional verification:

[0055] The bio-environment restoration solution prepared in Example 1 is carried out to verify the growth state of rice:

[0056] In the rice seedling stage, there are 2 experimental fields, each of which is 2 mu. One piece that only applies chemical fertilizer is the control group (only applies chemical fertilizer 70kg), and one piece adds the green algae active cell biological fertilizer of the control group 2 / 3 chemical fertilizer and 2L embodiment (applying into chemical fertilizer 46kg and 2L green algae active cell biological fertilizer) is small In the coccus group, observe and record the growth of rice after 20 days and record.

[0057]

[0058] Prepare 2 test fields, each with 3 mu. One piece with only chemical fertilizer applied is the control group (only 60kg of chemical fertilizer is applied), and one piece with 2 / 3 of the chemical fertilizer and 3L of green algae active cell biological fertilizer (applied with 40k...

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Abstract

The invention discloses chlorella pyrenoidosa, biological environment restoration liquid prepared therefrom and application of the chlorella pyrenoidosa, relates to a green alga active cell biologicalrestoration liquid, and aims to provide chlorella for environment restoration and application of the chlorella. The chlorella is the Chlorella pyrenoidosa ZXL X, and the depositary authority is ChinaCenter for Type Culture Collection, the depositary address is Wuhan University, Wuhan, Hubei Province, the depositary date is November 18, 2020, and the depositary number is CCTCC NO: M 2020760. Thechlorella pyrenoidosa is used for improving the soil environment, promoting crop yield increase and improving crop quality. The restoration liquid is prepared by the following steps: culturing chlorella pyrenoidosa with a nutrient solution to an exponential growth phase and mixing the cultured chlorella pyrenoidosa with an alginate solution; extruding the mixed solution to a calcium chloride solution to obtain globules; and mixing with the globules with a working solution to obtain the biological environment restoration liquid. The environment restoration liquid provided by the invention is high in content value, does not produce drug residues, and can realize agricultural sustainable development.

Description

technical field [0001] The invention relates to a liquid for bioremediation of active cells of green algae, in particular to the preparation and application of a liquid for restoration of the biological environment of active cells of green algae. Background technique [0002] The average soil organic matter is less than 1%, and it takes 100 years for a natural increase of 1%. Overexploitation causes vegetation damage, soil erosion, and ecological restoration may take more than a thousand years. The lack of organic matter on the soil surface, the imbalance of nutrient ratio, the lack of capillary pores in the soil, the poor ventilation and water permeability, and the poor activity of microorganisms cause the soil acidity and alkalinity to be too large or too small, resulting in poor water retention, fertilizer retention, and air permeability. Meet the actual growth needs of crops. In addition, industrial pollution has damaged the soil, aggravating the destruction of soil org...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/12C12N11/10C12N11/04C05G3/00C05G3/80C05G5/20C09K17/00A01N65/03A01P21/00C12R1/89C09K101/00
CPCC12N11/10C12N11/04C05B1/02C05G3/00C05G3/80C05G5/20C09K17/00A01N65/03C09K2101/00C05C3/00C05D1/02C05D5/00C05D9/00C05D9/02C05F11/00C05F11/08
Inventor 范秀娟段萃枝
Owner 范秀娟
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