Monitoring method for change of nitrogen stress of characteristic peaks of microalgal oil with time

Abstract

The invention discloses a monitoring method for the change of nitrogen stress of the characteristic peaks of microalgal oil with the time. The monitoring method comprises the following steps: (1) adopting a Raman spectrometer and acquiring Raman-spectrum original information of chlorella pyrenoidosa under the same nitrogen-nutrition environment at different periods; (2) preprocessing the obtained Raman-spectrum original information, obtaining a preprocessed spectrogram and extracting Raman intensity values corresponding to the microalgal characteristic peaks in the spectrogram; (3) adopting the Raman intensity values as input, adopting different periods of the same nitrogen-nutrition environment as output, and establishing a judgment model based on a multiple regression algorithm; and (4) taking live algae liquid, obtaining a corresponding Raman intensity value by processing in the step (1) and the step (2), inputting the judgment model and obtaining a result of the change of the nitrogen stress of the algae liquid to be monitored with the time. The monitoring method disclosed by the invention has the advantage that the problems that dyeing or complex chemical treatment needs to be carried out on the sample, the operation is relatively tedious and consumes time and labor in the existing detection method are solved.

Description

technical field [0001] The invention relates to the technical field of microalgae growth environment monitoring, in particular to a method for monitoring nitrogen stress over time based on characteristic peaks of microalgae oil. Background technique [0002] Microalgae are a class of eukaryotic microorganisms that can convert carbon dioxide into potential biofuels, food, feed, and high-value bioactive molecules and perform photosynthesis. Microalgae have ecological and biological value and are an important biomass resource. [0003] Chlorella is a type of single-celled green algae, which belongs to Chlorophyta, Chlorophyceae, Chlorophyceae, Softycetaceae, and Chlorella genus. It is widely distributed in nature and has the largest variety in freshwater waters. At present, there are about 15 species of chlorella known in the world, and there are more than a hundred varieties. Chlorella cells are generally spherical or ellipsoidal in shape, with a diameter of 2-12 μm. Studie...

AI Technical Summary

Problems solved by technology

[0005] The present invention provides a monitoring method of nitrogen stress over time based on the characteristic peaks of microalgae lipids, which solves the problems of relatively cumbersome, time-consuming, and labor-intensive operations that require dyeing or complex chemical treatment of samples in existing detection methods

By preprocessing the collected Raman signal, the Raman spectrum intensity value is easily affected by different growth stages of microalgae, different exposure times, and the decomposition of pigments over time.

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