A method for detecting chlorophyll b content in tea using confocal Raman microscopy

A confocal microscopy and chlorophyll technology, applied in Raman scattering, material excitation analysis, etc., can solve the problems of destructiveness, cumbersome detection steps, unstable chlorophyll properties, and inability to realize online detection, etc., and achieve good economic benefits. The effect of shortening the inspection time and simplifying the structure

Active Publication Date: 2018-12-28
ZHEJIANG UNIV
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  • Claims
  • Application Information

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Problems solved by technology

Traditional detection methods are destructive, the detection steps are cumbersome, and online detection cannot be realized
At the same time, the properties of chlorophyll in tea are not very stable, and are easily changed by light, oxygen, and heat during the experiment. The accuracy of traditional chemical methods for determining chlorophyll in tea is easily affected by experimental conditions and experimental operations.

Method used

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  • A method for detecting chlorophyll b content in tea using confocal Raman microscopy
  • A method for detecting chlorophyll b content in tea using confocal Raman microscopy
  • A method for detecting chlorophyll b content in tea using confocal Raman microscopy

Examples

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Embodiment 1

[0057] The sample stage structure provided by the present invention is as image 3 As shown, it includes: slide glass 2, cover glass 1 and stage 3. Both the slide glass 2 and the cover glass 1 are made of quartz.

[0058] The surface of slide glass 2 is used to place tea sample, and slide glass 2 is rectangular, and two long sides of slide glass 2 are respectively fixed with a slide block 7, and spring pin is fixed on each slide block 7, and two slide blocks 7 corresponding to the location of the spring pin on the A silicon chip 5 is embedded on the slide glass 2 .

[0059] The cover glass 1 is rectangular, and the long side of the cover glass 1 is hinged with one of the sliders 7, and the cover glass 1 is overturned to compress the tea sample. The cover glass 1 is used to keep the surface of the tea leaves flat, to minimize the variation of the object distance when detecting different points on the tea samples, and to ensure that the Raman detection lens can be accurately ...

Embodiment 2

[0062] Take 315 pieces of Longjing 43 leaves. For each Longjing 43 leaf, randomly cut the leaves on both sides of the veins to obtain two tea samples. The mass of each tea sample is about 0.1g. After cutting, the actual measurement and record Ww. One of them is directly placed in the bag and labeled as Raman spectroscopic material A, the other is cut into pieces and placed in a centrifuge tube and labeled, added with 10mL of alcohol solution with a mass fraction of 95%, and stored in a dark room for about 24 hours , as UV spectrophotometric material B. For the same tea leaves, Raman spectroscopic material A and UV spectrophotometric material B have the same label.

[0063] The Raman spectroscopic material A is fixed on the Raman stage described in Example 1, using a Renishaw confocal laser microscope Raman instrument (Renishaw in Via-Reflex 532 / XYZ), the excitation wavelength is 532nm; the laser intensity 50mW; integration time 1s; Raman spectrum detection wavelength range i...

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Abstract

The invention discloses a method for detecting the content of chlorophyll b in tea leaves with confocal micro-raman. The method comprises the following steps that firstly, a tea leaf sample is put on a sample table, a raman spectrometer is adopted to obtain raman spectrum information of the tea leaf sample, and full-waveband integral correction is carried out; secondly, a linear model is established with the raman spectrum strengths at 15 characteristic wave numbers; thirdly, corrected spectrum strengths, at 15 characteristic wave numbers, of the tea leaf sample to be detected are collected, the content of chlorophyll b in tea leaves is predicted with the linear model, and nondestructive and quick detection of the content of chlorophyll b in tea leaves is achieved. By means of the method, quick, nondestructive, low-cost and environment-friendly detection of content of chlorophyll b in tea leaves can be effectively achieved, complex pretreatment and chemical analysis do not need to be carried out on the tea leaf sample, the operation steps are greatly simplified, the detection time is shortened, the information of the chlorophyll b ingredient in tea leaves is quickly obtained, and the detection accuracy is improved.

Description

technical field [0001] The invention relates to the technical field of chlorophyll b content detection, in particular to a method for detecting chlorophyll b content in tea leaves by confocal microscopic Raman. Background technique [0002] The functional shape of plant leaves directly reflects the physiological growth and genetic characteristics of plants, and its changing trend affects the material and energy exchange between plants and the surrounding environment and the survival strategies formed by plants adapting to environmental changes. Leaf is the main place for plants to carry out photosynthesis, and photosynthesis is the energy conversion process of light energy into chemical energy, which is the basis of crop yield and quality. [0003] [Study on the functional traits and photosynthetic characteristics of leaves in different leaf positions of tea trees] points out that leaves are important organs of plant photosynthesis, solar energy is first absorbed by the pigm...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/65
CPCG01N21/65
Inventor 李晓丽许凯雯何勇周瑞清张裕莹魏玉震
Owner ZHEJIANG UNIV
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