Culture method for chlorella pyrenoidosa

A technology of Chlorella pyrenoidosa and a culture method, applied in the field of algae culture, can solve the problems of unfavorable large-scale culture, low oil content, low biomass and the like, and achieve the effects of promoting rapid proliferation, increasing oil content and high cell density

Active Publication Date: 2017-10-24
HEILONGJIANG UNIVERSITY OF SCIENCE AND TECHNOLOGY
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Problems solved by technology

However, the large-scale cultivation of Chlorella pyrenoidosa currently has the problems of sl

Method used

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  • Culture method for chlorella pyrenoidosa
  • Culture method for chlorella pyrenoidosa
  • Culture method for chlorella pyrenoidosa

Examples

Experimental program
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Embodiment 1

[0021] Example 1: Screening and community structure identification of Chlorella pyrenoidosa and mixed flora symbiotic system

[0022] The water samples extracted from the biochemical pool activated sludge of the Harbin Municipal Sewage Plant were put into sterile sampling bags and brought back to the laboratory. Microscopic observation found that there were many microorganisms and algae cells in the samples, and the corresponding microalgae and microalgae associated flora were tested. Enrichment, separation and screening. Put the raw water samples into the simulated biochemical pool reactor for continuous cultivation, place the reactor under natural light, mix with the pre-prepared simulated urban sewage, and aerate for a long time to keep the oxygen content at 6-8mg / L , cultured at 32°C, pH 7.5 COD maintained at about 400mg / L, TN at about 50mg / L, and TP at about 10mg / L. The simulated urban sewage is simulated by the improved BG11 medium, and its simulated sewage composition ...

Embodiment 2

[0028] Example 2: Compound flora and activity verification

[0029] Simulate the composition ratio of the photosynthetic bacterial microbial community Rhodobacter sp. and Rhodopseudomonas sp. in Example 1, and compound it with Chlorella pyrenoidosa. Among them, the specific species of Rhodobacter sp. is Rhodobacter capsulatus, and the specific species of Rhodopseudomonas sp. is Rhodopseudomonas palustris. Among them, the single bacteria (algae) are respectively activated with LB medium, and the cell concentration is adjusted after activation, while the added concentration of Chlorella pyrenoidosa is two orders of magnitude greater than the concentration of the two kinds of photosynthetic bacteria. The oil content detection method was described, and the effect of the compound flora on the oil content of Chlorella pyrenoidosa was analyzed. After the cultivation, the oil content of Chlorella pyrenoidosa was 35.43%, and the growth was stable, and the algae cell density reached 5×...

Embodiment 3

[0030] Example 3: Optimization of growth and oil content of Chlorella pyrenoidosa with different medium concentrations and bacterial flora concentrations

[0031] Use sterile water to configure the optimized medium of Chlorella pyrenoidosa with the following formula: peptone 300mg / L, KH 2 PO 4 16mg / L, MgSO 4 ·7H 2 O 60mg / L, CaCl 2 2H 2 O 30mg / L, ferric ammonium citrate 8mg / L, NaHCO 3 10mg / L, trace element solution 0.5ml / L; also includes auxin indole acetic acid 1.0mg / L and vitamin B 2 Solution 0.5mg / L. 10 mL of mixed flora fermentation liquid including Rhodobacter capsulatus and Rhodopseudomonas palustris, in which the inoculation ratio of the two strains is 1:1. The specific composition of its mixed flora fermentation liquid is as follows: NH 4 Cl1g / L, K 2 HPO 4 0.5g / L, MgCl 2 0.2g / L, yeast powder 0.5g / L, sodium acetate 4g / L. The fermentation temperature is 30°C, 24 hours of continuous light, the intensity is 10000Lux, the fermentation time is 3 days, the inocu...

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Abstract

The invention provides a culture method facilitating the increase of the growth rate of chlorella pyrenoidosa and cell density and the promotion of fat accumulation. Mixed bacterial fermentation liquid with inactivated Rhodobacter sp. and Rhodopseudomonas sp. is added in a culture medium for culturing chlorella pyrenoidosa. Through the adoption of the culture method, chlorella pyrenoidosa cells can grow quickly, and cultured chlorella pyrenoidosa is high in cell intensity; in particular, by adding the mixed bacterial liquid, the quick multiplication of the chlorella pyrenoidosa cells is promoted, the concentration of the chlorella pyrenoidosa cells is increased, the content of fat is obviously increased, and the method is suitable for mass and large-scale culture production.

Description

technical field [0001] The invention belongs to the technical field of algae cultivation, and in particular relates to a method for cultivating Chlorella pyrenoidosa. Background technique [0002] Chlorella pyrenoidosa belongs to Chlorella pyrenoidosa, Chlorella phylum, Chlorella genus, and is the only species in this genus with pyrenoid. Cells of Chlorella pyrenoidosa are rich in protein, polysaccharides, unsaturated fatty acids, dietary fiber, vitamins and trace elements, etc., and have high nutritional value. They are excellent health food and aquaculture bait for human beings. It has been approved as a new resource food by the Ministry of Health of the People's Republic of China. As a kind of renewable energy biomass, Chlorella pyrenoidosa can accumulate oil under photoautotrophic and heterotrophic culture conditions, and can effectively use solar energy and organic energy to grow rapidly and accumulate oil. The oil of Chlorella pyrenoidosa can As a renewable energy so...

Claims

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Application Information

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IPC IPC(8): C12N1/12C12N1/38C12R1/89
CPCC12N1/12C12N1/38
Inventor 李立欣宋志伟战友
Owner HEILONGJIANG UNIVERSITY OF SCIENCE AND TECHNOLOGY
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