Culture medium and culture method for culturing chlorella pyrenoidosa by sheep manure leach liquor

A technology of chlorella pyrenoidosa and leaching solution, applied in the direction of microorganism-based methods, biochemical equipment and methods, single-cell algae, etc., can solve the problems of time-consuming, laborious, immature technology, troublesome operation, etc.

Inactive Publication Date: 2014-12-17
LINYI UNIVERSITY
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  • Abstract
  • Description
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  • Application Information

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Problems solved by technology

[0008] However, the above traditional utilization methods of sheep manure have more or less disadvantages such as immature technology, low

Method used

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  • Culture medium and culture method for culturing chlorella pyrenoidosa by sheep manure leach liquor
  • Culture medium and culture method for culturing chlorella pyrenoidosa by sheep manure leach liquor

Examples

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Embodiment 2

[0050] Other steps are the same as in Example 1, the difference is that the disinfectant solution is made up of the following weight ratios: every 100 parts of 85% ethanol adds the following Chinese medicinal materials and auxiliary materials in parts by weight: 40 parts of Tujing bark, 20 parts of bar plate , 20 parts of Phellodendron, 10 parts of Sophora flavescens, 10 parts of Artemisia argyi leaves, 4 parts of auxiliary materials; the preparation method of disinfectant solution: chop the soil wattle bark, bark bark, Phellodendron flavescens, Sophora flavescens, and Artemisia argyi leaves separately, dry at 65°C, and then Grind to 70 meshes with a Chinese herbal medicine pulverizer for later use; respectively weigh 40 parts by weight of dried and pulverized Tujingpi, 20 parts of barbarum, 20 parts of Cortex Phellodendri, 10 parts of Sophora flavescens, and 10 parts of Artemisia argyi, and place After fully mixing in the Erlenmeyer flask, add 100 parts of 85% ethanol, soak fo...

Embodiment 3

[0056] Other steps are the same as in Example 1, the difference is that the disinfectant solution is made up of the following weight ratios: every 100 parts of 85% ethanol adds the following Chinese medicinal materials and auxiliary materials in parts by weight: 50 parts of Tujing skin, 25 parts of bar plate , 25 parts of Cortex Phellodendri, 15 parts of Sophora flavescens, 15 parts of Artemisia argyi leaves, 5 parts of auxiliary materials; the preparation method of disinfectant solution: chop the soil wattle bark, bark bark, Cortex flavescens, Sophora flavescens, and Artemisia argyi leaves respectively, dry them at 70°C, and then Grind them to 70 meshes with a Chinese herbal medicine pulverizer for later use; weigh 50 parts by weight of dried and pulverized Tujing bark, 25 parts of barbarum, 25 parts of Cortex Phellodendri, 15 parts of Sophora flavescens, and 15 parts of Artemisia argyi. After fully mixing in the Erlenmeyer flask, add 100 parts of 85% ethanol, soak for 48 hour...

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Abstract

The invention discloses a method for culturing chlorella pyrenoidosa by taking sheep manure leach liquor as a main nitrogen source, and belongs to the field of aquiculture. The chlorella pyrenoidosa is rich in nutrition, widely used for manufacturing healthcare products, cosmetics, medicines, sea fishes and shrimp feeds; a f/2 culture solution and an open cement pit are conventionally adopted to culture, so that the growth is slow, the pollution is easily produced, the cost is high and the benefits are low; at present, a horizontal type white plastic water storage tank is adopted for aerated feeding culture, and the sheep manure leach liquor is taken as the main nitrogen source and added with an appropriate amount of nutritional ingredients such as ammonium nitrate, sodium dihydrogen phosphate, soil extract, DL malic acid, D-sodium erythorbate, potassium silicate, indoleacetic acid, alpha-naphthylacetic acid, medical stone powder, a titanium solution, wheat gluten and hydroxypropyl methyl cellulose, so that not only can the waste be utilized, but also microalgae with a high healthcare value can be obtained; the organic fertilizer and the inorganic fertilizer are mixed for use, so that the nutrition is relatively comprehensive, the operation is convenient, the automatic and large-scale production can be realized, the cost is low, the benefits are high, and the microalgae yield is increased by 240%.

Description

technical field [0001] The invention belongs to the field of aquaculture, in particular to a method for cultivating Chlorella pyrenoidosa with sheep manure leaching solution. Background technique [0002] In recent years, with the rapid development of the sheep farming industry, more and more sheep farms have adopted large-scale, intensive, and factory-style farming, and the large amount of sheep manure produced has become one of the organic waste resources that need to be resolved urgently. Sheep manure contains pathogenic microorganisms, parasites, certain chemicals, toxic metals and hormones, etc. If it is not treated in a timely and scientific manner, it will not only deteriorate the sanitary environment of sheep farms, but also cause sheep to be infected with diseases. At the same time, the random discharge of manure will also cause environmental pollution , spread disease, and seriously endanger human health. Sheep dung is as big as soybeans, black, and nearly oval in...

Claims

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Application Information

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IPC IPC(8): C12N1/12C12R1/89
Inventor 王培磊
Owner LINYI UNIVERSITY
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