Micro-algae ball and immobilization method

A technology of microalgae algae balls, algae balls, applied in microorganism-based methods, biochemical equipment and methods, fixed on/in organic carriers, etc., can solve problems such as poor effect, high cost, secondary pollution and the like , to reduce costs, improve efficiency, and easy to collect

Inactive Publication Date: 2018-04-27
WUHAN JINGYU MICROALGAE SCI & TECH CO LTD +1
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  • Abstract
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  • Application Information

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Problems solved by technology

[0002] At present, there are mainly three methods of treating wastewater from large-scale livestock and poultry farms at home and abroad: physical method, chemical method and biological method, but these methods often have problems such as high cost, poor effect, and secondary pollution.

Method used

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  • Micro-algae ball and immobilization method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] The immobilization method of the microalgal algae provided in this embodiment comprises the following steps:

[0028] Step 1, the number of cells grown in a certain concentration of livestock and poultry breeding wastewater is 1-5×10 6 The chlorella pyrenoidosa solution of each / ml is centrifuged under the condition of 7000-8000rpm / min, and centrifuged for 3-5min. Remove the supernatant, wash 2-3 times with deionized water, and set the volume to 50-60ml to obtain the concentrated chlorella pyrenoidosa solution;

[0029] Step 2, the prepared concentrated chlorella pyrenoidosa solution and the sodium alginate solution with a mass concentration of 2% were mixed in a magnetic stirrer for 1-2 hours at a volume ratio of 1:2 to prepare a standby solution;

[0030] Step 3, drop the standby solution into the calcium chloride solution with a concentration of 2% at a uniform speed, the volume ratio of the standby solution to the calcium chloride solution is 1:4-6, crosslink for 6-...

Embodiment 2

[0035] In this example, except for step 2, other steps of the method for immobilizing microalgae algal bulbs are the same as in example 1, and the concentration of sodium alginate in step 2 is 3%.

Embodiment 3

[0037] In this example, except for step 2, the immobilization method and other steps of the application of microalgae algae bulbs are the same as in example 1, and the concentration of sodium alginate in step 2 is 4%.

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Abstract

The invention relates to a micro-algae ball and an immobilization method. The immobilization method includes the steps: (a) centrifuging chlorella pyrenoidosa solution with (1-5)*10<6> cells / ml in waste water to remove clear liquid, cleaning the chlorella pyrenoidosa solution for 2-3 times by the aid of deionized water, reaching the constant volume of 50-60ml to obtain concentrated chlorella pyrenoidosa solution; (b) uniformly mixing the prepared concentrated chlorella pyrenoidosa solution and sodium alga acid solution with the mass concentration of 2%-4% according to the volume ratio of 1:2 to prepare standby application solution; (c) dropping the prepared standby application solution into calcium chloride solution with the concentration of 2% at constant speed according to the volume ratio of 1:(4-6), and cross-linking mixture to form immobilization algae balls; (d) dissolving the obtained immobilization algae balls by the aid of sodium citrate solution with the concentration of 3%-5% according to the volume ratio of 1: 1 to prepare mixed solution; (e) centrifuging the obtained mixed solution to collect algae cells. According to the micro-algae ball and the immobilization method,the treatment efficiency of waste water can be effectively improved, cost is reduced, and immobilization algae balls are easily recovered.

Description

technical field [0001] The invention relates to the field of livestock and poultry wastewater treatment, in particular to a microalgae algae ball and an immobilization method. Background technique [0002] At present, there are mainly three methods of treating wastewater from large-scale livestock and poultry farms at home and abroad: physical method, chemical method and biological method, but these methods often have problems such as high cost, poor effect, and secondary pollution. [0003] In order to solve the above-mentioned technical problems, the present invention provides a microalgae algae ball and an immobilization method. The immobilized algae ball has a good purification effect on livestock and poultry breeding wastewater, and can fully meet the livestock and poultry breeding discharge standard; The algae balls are easy to collect, because microalgae are rich in various biologically active substances, the collected algae balls can be developed into functional feed...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/10C12R1/89
CPCC12N11/10
Inventor 王润发黄开耀
Owner WUHAN JINGYU MICROALGAE SCI & TECH CO LTD
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