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Preparation method for algae solution used for determining chlorophyll content through fluorescence method

A technology of chlorophyll content and fluorescence method, which is applied in the direction of measuring devices, fluorescence/phosphorescence, and material analysis through optical means, can solve the problems of inability to obtain algae liquid concentration data, easy sedimentation, etc., to ensure accuracy, reduce impact, The effect of improving suspension

Active Publication Date: 2015-11-11
HEBEI UNIVERSITY OF SCIENCE AND TECHNOLOGY
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Problems solved by technology

[0005] The present invention provides a method for preparing algae liquid used for measuring chlorophyll content by fluorescence method in order to solve the problem of easy sedimentation and inability to obtain accurate algae liquid concentration data when measuring algae liquid by fluorescence method in the prior art

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  • Preparation method for algae solution used for determining chlorophyll content through fluorescence method
  • Preparation method for algae solution used for determining chlorophyll content through fluorescence method
  • Preparation method for algae solution used for determining chlorophyll content through fluorescence method

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Embodiment Construction

[0016] The invention provides a method for preparing algae liquid used for measuring chlorophyll content by fluorescence method in order to solve the problem that algae are easy to settle and cannot obtain accurate algae liquid concentration data when measuring algae liquid by fluorescence method in the prior art. The present invention will be further described below in conjunction with specific examples.

[0017] 1. Experimental instruments and reagents

[0018] Instruments: light incubator (E-30B0 American Percival); visible spectrophotometer (722 Shanghai Spectrum Instrument Co., Ltd.); ultraviolet spectrophotometer (UV-2600 Shanghai Tianmei Scientific Instrument Co., Ltd.); fluorescence spectrophotometer (RF- 5301 Japan Shimadzu Corporation); centrifuge (Z323 German HERMLE-Z323); circulating water multi-purpose vacuum pump (SHB-Ⅲ Zhengzhou Great Wall Science, Industry and Trade Co., Ltd.).

[0019] Reagents: hydrochloric acid (analytical pure Shijiazhuang Huadi Chemical I...

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Abstract

The invention relates to a preparation method for an algae solution used for determining the chlorophyll content through a fluorescence method. The preparation method includes the steps that a chlorella pyrenoidosa species is selected, a BG-11 culture medium is prepared, sterilized, cooled and inoculated with the algae species under the aseptic condition, after inoculation is completed, the culture medium is placed in a constant-temperature illumination incubator to culture the algae species, and before the culture medium is sterilized, agar powder is added into the BG-11 culture medium to culture chlorella pyrenoidosa. According to the gelling property and stability of the agar powder, the preparation method for the algae solution is provided, the agar powder (0.08-0.10 g / 100ml) is added into the BG-11 culture medium to culture the chlorella pyrenoidosa as a suspending agent, the cultured chlorella pyrenoidosa is used for preparing the algae solution, the suspension property and stability of the algae solution are improved, and then the result accuracy of determining the chlorophyll content through the fluorescence method is ensured.

Description

technical field [0001] The invention belongs to the technical field of water quality environment monitoring, and in particular relates to a method for preparing algae liquid for measuring chlorophyll content by fluorescence method. Background technique [0002] The content of chlorophyll a reflects the quantity of algae and the water quality in the water body to a certain extent. It is a routine monitoring item in the water quality environment monitoring and one of the eutrophication indicators of the water body. [0003] laboratory spectrophotometry [1-2] , fluorescence spectrophotometry [3] It is the most commonly used method for laboratory determination of chlorophyll a to achieve quantitative determination of phytoplankton. Fluorescence photometry is used to measure chlorophyll a in water, and the sensitivity is 10 times higher than that of spectrophotometry. It does not require pretreatment of samples, is simple to operate, and can be continuously monitored. With the ...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64G01N21/84
Inventor 崔建升樊琳琳段莉丽高柳堂高思周盼
Owner HEBEI UNIVERSITY OF SCIENCE AND TECHNOLOGY
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