Cephalotaxus hainanensis embryo culturing and seedling breeding method

Abstract

The invention provides a cephalotaxus hainanensis embryo culturing and seedling breeding method which relates to a plant quick propagating and breeding method. The method comprises the following steps: (1) collection and selection of explants; (2) cleaning and sterilization of seeds and embryo obtainment; (3) inoculation and culture of embryos; (4) passage and root induction of test-tube seedlings; and (6) acclimatization and transplantation. The invention firstly creates the whole method of using cephalotaxus hainanensis embryos as explants to perform tissue culture and quick propagation, and provides high-quality culture mediums for breeding the cephalotaxus hainanensis embryos, thereby not only producing regular high-quality cephalotaxus hainanensis seedlings and providing a technical support for the quick propagation and restoration protection of rare or endangered species of cephalotaxus hainanensis and the development and the utilization of resources, but also laying foundation for further exploring gene engineering of cephalotaxus hainanensis, widening explant materials, and the like.

Description

technical field [0001] The invention relates to a method for rapid breeding of plants, in particular to a method for culturing seed embryos of Hainan rough torreya and rapidly breeding seedlings. Background technique [0002] Hainan rough torreya (Cephalotaxus hainanensis Li) is a plant of the family Cephalotaxus, aliased as Red Cattle Pine and Thin-leaved Castor Cedar, and is a perennial tall evergreen tree. It is a rare tree species unique to my country, and its distribution range is extremely narrow, only found in Hainan, Guangdong, Yunnan and other local areas. Studies have shown that not only the effective alkaloid content of Torreya hainanensis is the highest, but also the curative effect is the most significant. It is currently recognized as one of the best anti-cancer tree species. In addition, this tree species has been felled repeatedly due to its excellent material, and its population is small. It is one of the most important rare and endangered plants in my coun...

AI Technical Summary

Problems solved by technology

In addition, there are also people who have studied the use of traditional tissue culture methods to propagate seedlings of Torreya hainanensis, but basically no seedlings can emerge, or the emergence rate is very low, and application and promotion cannot be carried out (Fu Wenying, Du Daolin, Fu Mujun. Induction of callus of Torreya hainanensis and Cultivation. Communications of Plant Physiology, 2004, 40(1): 34-36; Li Zhiying, Wang Zhunian, Xu Li. Rapid Propagation of Torreya hainanensis in vitro. Communications of Plant Physiology, 2005, 41(5): 786)

[0003] The present invention provides a method of directly cultivating seed embryos of Torreya hainanensis for rapid propagation and seedling cultivation. In a relatively short period of time, only a small number of seeds can be used to produce a large number of uniform and high-quality seedlings, avoiding the problem of not being able to obtain a large number of seeds. It is very suitable for large-scale promotion to meet the urgent needs of Hainan rough torreya, a rare and endangered plant seedling.