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Method for producing Fujian jewel orchid by plant tissue culture

A technology of plant tissue culture and clematis, applied in the field of plant tissue culture, can solve the problems affecting the in-depth research and application of clematis in Fujian, and achieve the effects of maintaining drug efficacy, short cycle and low production cost.

Inactive Publication Date: 2011-01-26
FUJIAN GRASS KING BIOLOGICAL GRP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

An effective culture technique has not been found to solve the doubts and difficulties in the induction and proliferation of Fujian Daodi A.

Method used

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  • Method for producing Fujian jewel orchid by plant tissue culture
  • Method for producing Fujian jewel orchid by plant tissue culture

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Embodiment 1: a kind of method of producing Fujian Daodi Auranthus clematis by tissue culture, he comprises the steps:

[0026] A. Plant selection and aseptic treatment:

[0027] A) Select the wild Fujian authentic golden clematis plant grown in August, wash it with tap water for 2-3 hours, clean it with a hair brush, remove the roots and leaves, rinse it with distilled water, and put it in the clean workbench;

[0028] B) Soak the stem plant body from which roots and leaves have been removed with 75% alcohol for 30 seconds to sterilize the surface, and then clean it with sterile water;

[0029] C) Soak and sterilize the stem section plant body through alcohol sterilization with 0.5% sodium hypochlorite solution for 5 minutes, rinse 5 times with sterile water, then transfer to 0.15% mercuric solution for sterilization for 8 minutes, pour off the sterilizing solution, Rinse 4-5 times with sterile water prepared in advance, and then dry the sterile water with filter pape...

Embodiment 2

[0035] Embodiment 2: a kind of method of producing Fujian Daodi Auranthus clematis by tissue culture, it comprises the steps:

[0036] A. Plant selection and aseptic treatment:

[0037] A) Select the wild Fujian authentic golden clematis plant grown in October, wash it with tap water for 2-3 hours, clean it with a hair brush, remove the roots and leaves, rinse it with distilled water, and put it in the clean workbench;

[0038] B) Soak the stem plant body from which roots and leaves have been removed with 75% alcohol for 25 seconds to sterilize the surface, and then clean it with sterile water;

[0039] C) Soak and sterilize the stem section plant body through alcohol sterilization with 0.3% sodium hypochlorite solution for 5 minutes, rinse 4 times with sterile water, then transfer to 0.15% mercuric chloride solution for sterilization for 8 minutes, pour off the sterilizing solution, Rinse 4-5 times with sterile water prepared in advance, and then dry the sterile water with f...

Embodiment 3

[0045] Embodiment 3: Adopt MS culture medium, improved MS culture medium to carry out the culture experiment of inducing bunchy buds to the Fujian Daodi Auranthus clematis tissue after aseptic treatment respectively, this experiment is repeated three times, and culture condition is identical with embodiment 2, The number of differentiation, tissue fresh weight, differentiation rate and growth vigor of A. aureus were investigated experimentally. The test results are as follows:

[0046] Experimental comparison of different media and different concentrations of hormones on clustered buds:

[0047]

[0048] Note: + means the color is yellowish, ++ means the color is slightly yellow, +++ means better, ++++ means very green seedlings.

[0049] The test results prove that the present invention adopts two kinds of different basic medium and the induction medium added with different concentrations of auxin to cultivate the aseptically treated Fujian Daodi Aurantia clematis subsect...

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Abstract

The invention discloses a method for producing a Fujian jewel orchid by plant tissue culture, which comprises the following three procedures: selection and sterile treatment of plants, induced culture of cluster buds and large-scale culture. The method is technically characterized in that two different culture media are used in the induced culture procedure and the large-scale culture procedure, wherein the culture medium for the induced culture comprises an MS culture medium, sucrose of 10-50g / L, agar of 6-8g / L, activated carbon of 2.0-3.0g / L, naphthylacetic acid of 0.1-3.0mg / L and 6-benzylaminopurine of 0.1-5.0mg / L, and the pH value is 5.0-7.0; and the culture medium for the large-scale culture comprises a modified MS culture medium, calcium nitrate of 600mg / L, mashed banana of 30-50g / L, sucrose of 20-40g / L, agar of 6-8g / L, activated carbon of 2.0-3.0g / L, naphthylacetic acid of 1.0-2.0mg / L and 6-benzylaminopurine of 2.0-3.0mg / L, and the pH value is 5.0-7.0. Plant tissue culture can keep the good characters of the plants. The method has the advantages of easy operation, low production cost, high yield, good quality and no environment pollution, is suitable for industrial seedling raising and can realize mass production.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, in particular to a method for plant tissue culture production and tissue culture production of Fujian Daodi Auranthus chinensis. Background technique [0002] Fujian daodi Shorthairy Antenoron (Fujian daodi Shorthairy Antenoron) is a plant of the Orchidaceae family, and it is a kind of endangered and precious medicinal whole herb, and it is a traditional precious medicinal material in our country. It is flat in nature and sweet in taste, has the effects of clearing heat, cooling blood, dispelling wind and dampness, strengthening the heart and diuresis, strengthening the kidney and calming the liver, and lowering blood pressure. It is mainly used for hemoptysis, bronchitis, nephritis, cystitis, diabetes, hematuria, and rheumatoid arthritis. , Convulsions in children, poisonous snake bites, etc. Known as "the king of medicine". Because the remarkable medicinal efficacy and health car...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 陈咏梅黄碧华
Owner FUJIAN GRASS KING BIOLOGICAL GRP
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