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Method for regenerating plant from arundo donax linn callus

A technology for callus and plant regeneration, which is applied in the field of plant tissue culture, can solve the problem that the callus regeneration system is not seen, and achieve the effect of efficient regeneration

Active Publication Date: 2013-08-07
郎溪品旭科技发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Ran et al. (1998) reported the research on tissue culture and rapid propagation of Phyllostachys arundis, but there are no relevant reports and patent applications for the callus regeneration system.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] A method for regenerating plants from reed bamboo callus, comprising the steps of:

[0023] (1) Induction of callus: remove the leaves, base tissue and young upper tissue of the aseptic seedlings of Arundis, cut the aseptic tissues of Arundis into small pieces and inoculate them in 2,4-D1.0mg / l medium , the length of each small piece is about 0.3 cm, the basic medium is MS medium, the culture temperature is 25±1°C, after inoculation, the culture is dark for 3 days, and then the photoperiod is 16 hours light and 8 hours dark, and the light intensity is 2000Lx under cultivation. About 1 week before induction, the straight stems begin to bend and the epidermis becomes loose. Around 20 days, a small amount of granular callus grew on the stem. After 2 months, piles of callus can be found, but the healing rate is about 30%;

[0024] (2) Subculture of callus: take the granular milky white callus, cut it into small pieces and inoculate it in the medium containing 2,4-D5.0mg / ...

Embodiment 2

[0030] A method for regenerating plants from reed bamboo callus, comprising the steps of:

[0031] (1) Induction of callus: remove the leaves, basal tissue and young upper tissue of the aseptic seedlings of Arundis, cut the aseptic tissues of Arundis into small pieces and inoculate them in 2,4-D2.0mg / l medium , the length of each small piece is about 0.3 cm, the basic medium is MS medium, the culture temperature is 25±1°C, after inoculation, the culture is dark for 3 days, and then the photoperiod is 16 hours light and 8 hours dark, and the light intensity is 2000Lx Under culture, after 2 months, a callus is formed, and the healing rate is about 70%;

[0032] (2) Subculture of callus: take the granular milky white callus, cut it into small pieces and inoculate it in the medium containing 2,4-D5.0mg / l, the length of each small piece is about 0.5 cm, carry out subculture, repeat subculture once a month, the basic medium and culture conditions of subculture are the same as the b...

Embodiment 3

[0038] A method for regenerating plants from reed bamboo callus, comprising the steps of:

[0039] (1) Induction of callus: remove the leaves, basal tissue and young upper tissue of the aseptic seedlings of Arundis, cut the aseptic tissues of Arundis into small pieces and inoculate them in 2,4-D4.0mg / l medium , the length of each small piece is about 0.3 cm, the basic medium is MS medium, the culture temperature is 25±1°C, after inoculation, the culture is dark for 3 days, and then the photoperiod is 16 hours light and 8 hours dark, and the light intensity is 2000Lx Under culture, after 2 months, a callus is formed, and the callus is light yellow, milky white, white and other colors, and the granular milky white callus accounts for about 60-70%, and the healing rate is 100%;

[0040] (2) Subculture of callus: take the granular milky white callus, cut it into small pieces and inoculate it in the medium containing 2,4-D5.0mg / l, the length of each small piece is about 0.5 cm, ca...

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PUM

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Abstract

The invention relates to a method for regenerating plant fromarundo donax linn callus. The method comprises the following steps of (1) inducing callus: inoculating arundo donax linn tissue into culture medium containing1.0-5.0mg / l of 2,4-D (2,4-dichlorophenoxyacetic acid), carrying out dark cultivation for 3 days after inoculating, and then cultivating under the condition that the light period is 16 hours of illumination and 8 hours of darkness, and the illumination intensity is 2000Lx; (2) succesive transfer culture of callus: inoculating the callus into the culture medium containing 5.0mg / l of 2,4-D; (3) regenerating the callus plant: taking MS culture medium as a basic culture medium, and adding 0.5mg / L of IBA (indolebutyric acid) and 5.0mg / L of 6-BA (6-benzylaminopurine); (4) sprouting cultivation; (5) rooting cultivation: inoculating the plant into the MS culture medium in which 0-0.5mg / L of NAA (naphthylacetic acid) is added to root after sprouting; and (6) transplanting. A method for regenerating the plant from arundo donax linn callus is built by the invention; and expanding propagation of excellent arundo donax linn is facilitated.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and in particular relates to a method for regenerating plants from a reed bamboo callus. Background technique [0002] Arundo donax Linn., belonging to monocotyledonous plants, is a perennial evergreen tall herbaceous plant in the genus Arundo donax Linn. The planting of reed bamboo can not only fix the soil and protect the embankment, but also beautify and protect the ecological environment of the wetland. Guo et al. (2011) showed that Phyllostachys arundis has certain stabilizing and removing effects on As, Cd, Pb and Zn in polluted soil. Han et al. (2005) conducted a study on the use of Phyllostachys arundis to remediate wetlands polluted by heavy metals. [0003] The research and application of plants including Arundis to remediate pollution is a hotspot now. However, it is difficult to find super pollution remediation plants in nature. In order to obtain the ideal restoration...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 杨志红张丽娜田前进韩志萍
Owner 郎溪品旭科技发展有限公司
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