Method for regenerating plant from arundo donax linn callus

Abstract

The invention relates to a method for regenerating plant fromarundo donax linn callus. The method comprises the following steps of (1) inducing callus: inoculating arundo donax linn tissue into culture medium containing1.0-5.0mg / l of 2,4-D (2,4-dichlorophenoxyacetic acid), carrying out dark cultivation for 3 days after inoculating, and then cultivating under the condition that the light period is 16 hours of illumination and 8 hours of darkness, and the illumination intensity is 2000Lx; (2) succesive transfer culture of callus: inoculating the callus into the culture medium containing 5.0mg / l of 2,4-D; (3) regenerating the callus plant: taking MS culture medium as a basic culture medium, and adding 0.5mg / L of IBA (indolebutyric acid) and 5.0mg / L of 6-BA (6-benzylaminopurine); (4) sprouting cultivation; (5) rooting cultivation: inoculating the plant into the MS culture medium in which 0-0.5mg / L of NAA (naphthylacetic acid) is added to root after sprouting; and (6) transplanting. A method for regenerating the plant from arundo donax linn callus is built by the invention; and expanding propagation of excellent arundo donax linn is facilitated.

Description

technical field

[0001] The invention belongs to the technical field of plant tissue culture, and in particular relates to a method for regenerating plants from a reed bamboo callus. Background technique

[0002] Arundo donax Linn., belonging to monocotyledonous plants, is a perennial evergreen tall herbaceous plant in the genus Arundo donax Linn. The planting of reed bamboo can not only fix the soil and protect the embankment, but also beautify and protect the ecological environment of the wetland. Guo et al. (2011) showed that Phyllostachys arundis has certain stabilizing and removing effects on As, Cd, Pb and Zn in polluted soil. Han et al. (2005) conducted a study on the use of Phyllostachys arundis to remediate wetlands polluted by heavy metals.

[0003] The research and application of plants including Arundis to remediate pollution is a hotspot now. However, it is difficult to find super pollution remediation plants in nature. In order to obtain the ideal restoration...