Cherry rootstock tissue culture medium and improvement method of culture medium

A technology of tissue culture medium and cherry, applied in the field of agricultural biology, can solve the problems of vitrification, affecting production yield, and difficulty in rooting, etc., and achieve the effect of good growth, large leaves and strong stems

Inactive Publication Date: 2011-06-15
天津樱桃谷农业科技发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It is difficult to take root using conventional layering and hardwood cuttings. At present, tissue culture is mainly used in my country for breeding, but dwarf rootsto

Method used

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  • Cherry rootstock tissue culture medium and improvement method of culture medium
  • Cherry rootstock tissue culture medium and improvement method of culture medium
  • Cherry rootstock tissue culture medium and improvement method of culture medium

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] 1. The specific operation of preparing optimized medium mother solution

[0040]

[0041]

[0042] Macroelement No. 1: When preparing, weigh out and dissolve separately according to the order listed in the table, then mix them together in order, and finally add distilled water to make the total amount reach 2 liters.

[0043] Macroelement No. 2: Weigh and dissolve according to the amount in the table, and finally add distilled water to make the total amount reach 1 liter.

[0044] No. 3 iron salt: Iron salt should be prepared separately. Ferrous sulfate (FeSO 1 ·7H 2 O) 2.78 grams and 3.73 grams of disodium ethylenediaminetetraacetic acid (Na-EDTA) are dissolved in 1 liter of water, and are stored in a brown glass bottle after the preparation is completed.

[0045] No. 4 trace element: due to the small amount, for the sake of accurate weighing, it should be weighed one by one with an electronic balance, dissolved and mixed together one by one to make 1L mother ...

Embodiment 2

[0063] Effect comparison of the present invention: the operation steps and culture conditions of each stage are the same as the above-mentioned steps, and the data shown in the following tables only represent the different single variable conditions of the culture medium components used. (The light intensity is 3000lx, the light lasts for 12 hours a day, and the indoor humidity is kept at 70%-80%)

[0064] (1)

[0065]

[0066] (2)

[0067]

[0068]

[0069] (3)

[0070]

[0071] (4)

[0072]

[0073]

[0074] (5) transitional transplanting: adopt the conventional method to directly transfer the tissue culture rootstock that has completed rooting and hardening into a hole tray equipped with nutrient soil, keep the temperature in the hardening greenhouse between 15-30 degrees Celsius, and keep the indoor humidity at 50% --80%, according to the adaptability of the seedlings, the weak natural light is gradually strengthened, and the ventilation is gradually i...

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PUM

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Abstract

The invention discloses a cherry rootstock tissue culture medium, which is prepared from 2 to 60 ml/L of optimized MS base mother solution, 2545 g/L of sucrose, 6.5 to 7.0 g/L of agar, 0.4 to 0.6 mg/L of 6-Benzylaminopurine, 0.1 to 0.3 mg/L of indolebutyric acid, 0.061 to 0.067 mg/L of VC, 0.05 to1.00 mg/L of indoleacetic acid and 1000 to1200 mg/L of active carbon; the culture medium is specifically applied to the growth and cultivation of the bud of the dwarf cherry rootstock, the cultivation of the strong stock and the cultivation of the induced root; the proliferation and root-growing effect is excellent so that the root-growing rate of the dwarf cherry rootstock can reach 95% to 98%; the root is rough and tidy; the culture bud is healthy; the leaf is green, therefore, the labor and material are effectively saved; the survival rate of the transplant can reach 95%, and the foundation is established for the large-scale production. The tissue culture medium method disclosed by the invention is simple and easy, thereby simplifying the program, improving the efficiency, and reducing the cost. In the invention, the problems of brown stain and vitrification in the culture process of tissue culture medium can be solved; and therefore, the invention can be applied to the fast reproduction of the Gisela sweet dwarf cherry rootstock in the large-scale production.

Description

technical field [0001] The invention belongs to the field of agricultural biotechnology, and relates to a method for promoting plant regeneration through tissue culture technology, in particular to a cherry rootstock tissue culture medium and a culture medium improvement method. Background technique [0002] Cherry belongs to Rosaceae deciduous tree fruit tree. When mature, the color is bright red, exquisite and translucent, beautiful in taste and shape, graceful in shape, rich in sugar and iron, rich in nutrition, and is an important fruit tree and landscaping tree. It also has high edible and medicinal properties. In recent years, it has been widely cultivated in my country and has great potential for development. [0003] At present, the production of cherries mainly adopts cuttings, layering and root tiller propagation, which not only have a low reproduction coefficient, but also cause variety degradation and spread of diseases and insect pests. The use of tissue culture...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 江文肖宝祥罗飞杨扬
Owner 天津樱桃谷农业科技发展有限公司
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