Cherry rootstock tissue culture medium and improvement method of culture medium

Abstract

The invention discloses a cherry rootstock tissue culture medium, which is prepared from 2 to 60 ml / L of optimized MS base mother solution, 2545 g / L of sucrose, 6.5 to 7.0 g / L of agar, 0.4 to 0.6 mg / L of 6-Benzylaminopurine, 0.1 to 0.3 mg / L of indolebutyric acid, 0.061 to 0.067 mg / L of VC, 0.05 to1.00 mg / L of indoleacetic acid and 1000 to1200 mg / L of active carbon; the culture medium is specifically applied to the growth and cultivation of the bud of the dwarf cherry rootstock, the cultivation of the strong stock and the cultivation of the induced root; the proliferation and root-growing effect is excellent so that the root-growing rate of the dwarf cherry rootstock can reach 95% to 98%; the root is rough and tidy; the culture bud is healthy; the leaf is green, therefore, the labor and material are effectively saved; the survival rate of the transplant can reach 95%, and the foundation is established for the large-scale production. The tissue culture medium method disclosed by the invention is simple and easy, thereby simplifying the program, improving the efficiency, and reducing the cost. In the invention, the problems of brown stain and vitrification in the culture process of tissue culture medium can be solved; and therefore, the invention can be applied to the fast reproduction of the Gisela sweet dwarf cherry rootstock in the large-scale production.

Description

technical field [0001] The invention belongs to the field of agricultural biotechnology, and relates to a method for promoting plant regeneration through tissue culture technology, in particular to a cherry rootstock tissue culture medium and a culture medium improvement method. Background technique [0002] Cherry belongs to Rosaceae deciduous tree fruit tree. When mature, the color is bright red, exquisite and translucent, beautiful in taste and shape, graceful in shape, rich in sugar and iron, rich in nutrition, and is an important fruit tree and landscaping tree. It also has high edible and medicinal properties. In recent years, it has been widely cultivated in my country and has great potential for development. [0003] At present, the production of cherries mainly adopts cuttings, layering and root tiller propagation, which not only have a low reproduction coefficient, but also cause variety degradation and spread of diseases and insect pests. The use of tissue culture...

AI Technical Summary

Problems solved by technology

It is difficult to take root using conventional layering and hardwood cuttings. At present, tissue culture is mainly used in my country for breeding, but dwarf rootstocks of Gisela sweet cherry still have fatal problems such as browning and vitrification in the tissue culture process, which have repeatedly affected production output

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