The invention discloses preparation of a
sodium caseinate substitute and application of the
sodium caseinate substitute in a
plant-based coconut juice beverage, and belongs to the technical field of
plant protein processing. The method comprises the following steps: mixing bean flour with water, then inoculating compound
bacteria for
fermentation, and after the
fermentation is finished,
drying to stop the
fermentation to prepare the fermented bean flour, then carrying out gradient washing by using
ethanol solutions with different concentrations, filtering and
drying to obtain
alcohol-washed bean flour;
protein is extracted through an alkali-solution and acid-isolation method, and
protein isolate is prepared through redissolution and freeze-
drying; and finally, stirring, homogenizing and sterilizing the
protein isolate, the freshly squeezed coconut juice,
cane sugar and other ingredients to prepare the
plant-based coconut juice beverage. A large amount of volatile beany
flavor substances such as aldehydes and alcohols are effectively degraded and removed through the processes such as compound bacterium suspension fermentation and gradient
ethanol washing, the
nitrogen dissolution index and the emulsification characteristic of the bean protein are synchronously improved, and the prepared pure plant-based coconut juice has the characteristics of no beany
flavor, good
flavor and fine taste.