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Method for overcoming yellowing of leaves of tissue culture seedlings of Rosa damascena

A technology for Martens leather and tissue culture seedlings, applied in the field of plant tissue culture, can solve the problems of quality decline and tissue culture seedling leaves yellowing, and achieve the effects of reducing leaf yellowing, promoting rooting and reducing pollution rate.

Active Publication Date: 2011-04-27
DASHUN INT FLOWER
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Problems solved by technology

[0004] The invention provides a method for overcoming the yellowing of the leaves of the tissue-cultured seedlings of Damascus to solve the technical problem that the leaves of the tissue-cultured seedlings of Damascus are turned yellow during the propagation process of Rose Damascus, and improves the quality of tissue culture of Roses Damascus. seedling quality

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  • Method for overcoming yellowing of leaves of tissue culture seedlings of Rosa damascena

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Embodiment Construction

[0009] This experiment was divided into a test group and a control group, each with 50 explants.

[0010] Experimental method of the test group: Take the stem section of Damascus rose with axillary buds and soak it in 75% alcohol for 30 seconds, rinse it once with sterile water, and then use 2% sodium hypochlorite and 500mg / L carbenicen Soak and disinfect each element solution for 15 minutes, and then rinse with sterile water 3 to 5 times. After the stems are sterilized, they are inoculated on the MS medium with 30g of sucrose per liter and 6g of agar for the primary culture; the seedlings obtained from the primary culture are cut into sections, each with a leaf, and transferred to the multiplication culture with different concentrations of additives Subculture (additional concentration see Table 1). Subculture adopts interval culture, that is, after subculture twice on the proliferation medium, then transfer to MS medium without adding any hormones for subculture once, and t...

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Abstract

The invention provides a method for overcoming yellowing of leaves of tissue culture seedlings of Rosa damascena. The method comprises: taking rosa damascena stem segments with axillary buds as explants to be sterilized, inoculating the sterilized stem segments into an MS (Murashige and Skoog) culture medium to be subject to primary culture; and cutting the seedlings obtained after primary culture into segments and transferring the seedling segments to a multiplication culture medium to be subject to subculture at intervals, wherein 30g of cane sugar and 6g of agar are added to each litre of the MS culture medium; each seedling segment is provided with a leaf; the multiplication culture medium is the MS culture medium to each litre of which 0.5-4.0mg of 6-BA (6-benzylaminopurine), 0.5-2.0mg of KT (kinetin), 0.2mg of NAA (1-Naphthaleneacetic acid), 5-20mg of ABA (abscisic acid), 1-10mg of AgNO3, 30g of cane sugar and 6g of agar are added; and the primary culture and the subculture are carried out under the following conditions: the illumination intensity is 1500-2000Lx, the temperature is 23+ / -2 DEG C, and the humidity is 75-80%.

Description

technical field [0001] The present invention relates to the field of plant tissue culture method; especially the field of woody plant tissue culture method. Background technique [0002] Rosa damascena is a shrub. Branchlets have thick hooked prickles, sometimes mixed with bristles. Leaflets ovate, ovate-oblong, apex pointed, base subrounded, margin single-serrate, aglandular, upper glabrous, lower pilose; stipules sometimes grate-toothed, mostly adnate to petiole . 6-12 flowers, arranged in corymbs; pedicel slender, with glandular hairs; flower diameter 3-5 cm; calyx tube with glandular hairs, sepals ovate-lanceolate, outer glandular hairs; inner surface densely soft Hairy; petals pinkish; styles detached, hairy. Fruit pear-shaped or obovoid, red, often with bristles. [0003] It is native to Asia Minor and has been cultivated in southern Europe for a long time. It is used as raw material for incense making and has high economic value. It has been introduced and culti...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 杨铁顺
Owner DASHUN INT FLOWER
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