Tissue culture and rapid propagation technology of haworthia magnifica v .atrofusca

A technology for tissue culture, fast propagation and watermelon, which is applied in plant regeneration, horticulture, horticultural methods, etc., can solve the problems of slow reproduction speed, low reproduction coefficient, slow growth speed, etc., and achieves the effect of solving slow reproduction speed.

Pending Publication Date: 2021-02-05
CHUZHOU VOCATIONAL & TECHN COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Therefore, the purpose of the present invention is to provide the tissue culture rapid propagation technology of watermelon life, adopt the method for tissue culture to replace the division propagation on the market, the method for sowing propagation and leaf cutting propagation, effectively solve the problem that the traditional method has slow propagation speed, Problems such as low reproduction coefficient and slow growth rate

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0018] Embodiment comprises the steps:

[0019] a Material collection: Watermelon Shou takes the whole young inflorescence as material when the flower buds have not yet unfolded;

[0020] b Explant treatment and sterilization: (1) Explant treatment: Separate flower buds and flower stalks one by one, and then rinse under running water for 2 hours; (2) Disinfection method: Rinse twice with sterile water in an ultra-clean workbench Finally, wash with 75% ethanol shaking for 30s, and then rinse twice with sterile water; sterilize with 0.1% mercuric chloride for 10 minutes, then rinse with sterile water for 6 times, and dry the water with filter paper;

[0021] c callus induction: inoculate the sterilized material in MS+6-BA (5mg / L)+NAA (0.2mg / L) 30g / L, sucrose 30g / L, carrageenan 5.5g / L, pH value 5.8 The callus was successfully induced on the induction medium;

[0022] d Adventitious bud induction: Inoculate the sterilized material on the medium of MS+6-BA (3.0mg / L)+NAA (0.5mg / L)...

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PUM

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Abstract

The invention belongs to the technical field of tissue culture and rapid propagation, and particularly relates to a tissue culture and rapid propagation technology of haworthia magnifica v .atrofusca.The technology comprises the steps of material taking, explant treatment and sterilization, callus induction, adventitious bud induction, propagation and proliferation, rooting culture, seedling hardening and transplanting. The tissue culture method can replace methods of division propagation, sowing propagation and leaf cutting propagation on the market, and the problems that a traditional method is low in propagation speed, low in propagation coefficient, low in growth speed and the like are effectively solved.

Description

technical field [0001] The invention relates to the technical field of tissue culture rapid propagation, in particular to the tissue culture rapid propagation technology of watermelon longevity. Background technique [0002] It is a perennial herb of the genus Haworthia of the Liliaceae family, native to South Africa. Most longevity leaves are closely arranged in a rosette shape with linear veins. Watermelon Shou (Haworthia magnifica v.atrofusca) is a variety of Hongwenshou tissue culture variation, belonging to sclerophyllous plants, presenting transparent or translucent windows, loose racemes, and white florets. [0003] Currently on the market are mostly division propagation, sowing propagation and leaf cutting propagation, and these methods all have problems such as slow propagation speed, low reproduction coefficient, and slow growth rate. To this end, it is necessary to develop corresponding technical solutions to solve them. Contents of the invention [0004] The...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 姜自红贾雪晴江生泉涂清芳张维扬
Owner CHUZHOU VOCATIONAL & TECHN COLLEGE
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