Rapid propagation method for excellent Chinese fir family seedlings

A kind of family, excellent technology, applied in the field of Chinese fir cultivation, can solve the problems of reducing the breeding cost, easy browning, shortening the culture period, etc., and achieves the effects of reducing the breeding cost, improving the multiplication coefficient, and shortening the culture period

Pending Publication Date: 2021-07-13
贵州省林业科学研究院
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In view of this, the purpose of the present invention is to provide a kind of Chinese fir excellent family seedling rapid propagation method, solve the unstable genetic quality of Chinese fir tissue culture seedlings, the difficult problem of initial bud induction, easy browning, no rooting, simultaneously rooting, hardening and The transplanting procedure is simplified, the cultivation period is shortened, and the breeding cost is reduced

Method used

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  • Rapid propagation method for excellent Chinese fir family seedlings
  • Rapid propagation method for excellent Chinese fir family seedlings
  • Rapid propagation method for excellent Chinese fir family seedlings

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1: Preparation of induction medium

[0031] To prepare the induction medium, the anti-browning solution should be prepared first. The raw materials of the anti-browning solution were weighed according to the weight in Table 1, and then the anti-browning solutions of group 1-comparison group 8 were prepared respectively according to the preparation method of the anti-browning solution.

[0032] Table 1 (unit: g)

[0033]

[0034]

[0035] The preparation method of anti-browning liquid is as follows:

[0036] Heat the distilled water to boiling, take it out after boiling for 40 minutes, cut off oxygen and naturally cool to room temperature to obtain cold distilled water for use;

[0037] Sterilize the fresh purslane with alcohol, wash it with distilled water, dry it, cut it into pieces, freeze-dry it at a low temperature of 13pa and -20°C for 6 hours, grind it into powder after drying, add the prepared cold distilled water, and stir evenly Afterwards, stan...

Embodiment 2

[0053] Embodiment 2: rapid propagation of Chinese fir

[0054] (1) Grafting of excellent families: select the top-growing branches of the excellent families as scions, graft them on biennial Chinese fir container seedlings, and spray grafting with 0.5mg / L auxin, 0.2mg / L cytokinin and foliar fertilizer Seedlings, to accelerate the growth of grafted scions of excellent families, cut off the top tips after the scions have branched, to increase the number of branches of fine Chinese fir families, and then graft the branches of fine Chinese fir families that have survived the grafting twice to ensure the uprightness of fine Chinese fir families Growth, the grafting technique adopted is the conventional technique of grafting Chinese fir container seedlings, such as cutting, tongue, abdomen, etc.;

[0055] (2) Disinfection of explants: Collect the scions of excellent Chinese fir families after repeated grafting as explants, rinse the stem explants with tap water and place them in a r...

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Abstract

The invention discloses a rapid propagation method for excellent Chinese fir family seedlings, and belongs to the technical field of Chinese fir cultivation. The rapid propagation method comprises the following steps: (1) excellent family grafting, (2) explant disinfection, (3) induction culture, (4) multiplication culture, (5) strong seedling culture, (6) ex-vitro rooting seedling transplanting and the like. The rapid propagation method solves the problems that Chinese fir tissue culture seedlings have unstable genetic quality and initial buds are difficult to induce, prone to browning and free of rooting, meanwhile, the procedures of rooting, seedling hardening and transplanting are simplified, so that the culture period is shortened, and the breeding cost is reduced.

Description

technical field [0001] The invention relates to the technical field of Chinese fir cultivation, in particular to a method for rapid propagation of seedlings of fine Chinese fir families. Background technique [0002] Chinese fir is a unique and important timber tree species in my country. Its afforestation area and stock volume rank first in my country's artificial forests, and it occupies a very important position in forest resources and forestry production in southern China. In order to promote the rapid development of high-quality, high-efficiency, fast-growing Chinese fir plantation industry, the key is to apply "good varieties and good methods". At present, a large number of research results have been obtained in the breeding and genetic improvement of Chinese fir varieties. In the National Chinese fir breeding base of Dongfeng Forest Farm, Liping County, Guizhou Province, a number of excellent families adapted to the mountainous areas of Guizhou have been selected and ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G2/30A01H4/00
CPCA01G2/30A01H4/008A01H4/00
Inventor 娄丽陈波涛龙倩付品罗敬丁访军朱佳敏田小琴
Owner 贵州省林业科学研究院
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