Rapid propagation method for excellent Chinese fir family seedlings
A kind of family, excellent technology, applied in the field of Chinese fir cultivation, can solve the problems of reducing the breeding cost, easy browning, shortening the culture period, etc., and achieves the effects of reducing the breeding cost, improving the multiplication coefficient, and shortening the culture period
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Embodiment 1
[0030] Example 1: Preparation of induction medium
[0031] To prepare the induction medium, the anti-browning solution should be prepared first. The raw materials of the anti-browning solution were weighed according to the weight in Table 1, and then the anti-browning solutions of group 1-comparison group 8 were prepared respectively according to the preparation method of the anti-browning solution.
[0032] Table 1 (unit: g)
[0033]
[0034]
[0035] The preparation method of anti-browning liquid is as follows:
[0036] Heat the distilled water to boiling, take it out after boiling for 40 minutes, cut off oxygen and naturally cool to room temperature to obtain cold distilled water for use;
[0037] Sterilize the fresh purslane with alcohol, wash it with distilled water, dry it, cut it into pieces, freeze-dry it at a low temperature of 13pa and -20°C for 6 hours, grind it into powder after drying, add the prepared cold distilled water, and stir evenly Afterwards, stan...
Embodiment 2
[0053] Embodiment 2: rapid propagation of Chinese fir
[0054] (1) Grafting of excellent families: select the top-growing branches of the excellent families as scions, graft them on biennial Chinese fir container seedlings, and spray grafting with 0.5mg / L auxin, 0.2mg / L cytokinin and foliar fertilizer Seedlings, to accelerate the growth of grafted scions of excellent families, cut off the top tips after the scions have branched, to increase the number of branches of fine Chinese fir families, and then graft the branches of fine Chinese fir families that have survived the grafting twice to ensure the uprightness of fine Chinese fir families Growth, the grafting technique adopted is the conventional technique of grafting Chinese fir container seedlings, such as cutting, tongue, abdomen, etc.;
[0055] (2) Disinfection of explants: Collect the scions of excellent Chinese fir families after repeated grafting as explants, rinse the stem explants with tap water and place them in a r...
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