A method for fast breeding seedlings by tissue culture of mature embryos of fine chestnut
A technology for tissue culture and rapid propagation of seedlings, which is applied in horticultural methods, botany equipment and methods, plant regeneration, etc., can solve problems such as the inability to truly realize efficient and rapid propagation of chestnut seedlings, the impact of germination rate, and increase investment in tissue culture. Shorten the breeding cycle, ensure the germination rate, and solve the effect of rooting difficulties
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Embodiment 1
[0045]In this example, "花香" is used as an example, for explaining a method of culturing a fast-breeding seedlings according to the high-rated chestnut maturation of the present invention.
[0046]Includes the following steps:
[0047](1) The choice of high-rated chestnuts: Select the pepper, full, size, and no pathogenic "花香" chestnut, refrigerated in the refrigerator in the refrigerator in the refrigerator in the refrigerator;figure 1 The appearance of the selected chestnut;
[0048](2) Chestnut cleaning, disinfection: Choose fresh, healthy, free chestnut seeds rinse 20-30 min under the water (gently shaking or brush with brush), and eliminate floating seeds; In the net workbench, first use 75% alcohol soaked for 2min (soaking process needs to be continuously swayed to make the seed surface with disinfectant, the same), the sterile water is washed 2 times, then use 5% sodium sodium sodium sodium sodium 6min, sterile Water washing 3 times, use sterilized water absorbing paper to dry the surf...
Embodiment 2
[0056]The difference from the first embodiment is that the step (2), (3) the sterilization method of chestnut seeds and chestnut fruit.
[0057]details as follows:
[0058]Step (2):
[0059]Chestnut cleaning, disinfection: choose fresh, healthy, free chestnut seeds rinse 20-30 min under the water (gently shaking or brush with brush), and eliminate floating seeds; In this first, first use 75% alcohol soaked 2min (soaking process needs to make the seed surface with disinfectant), sterile water is washed 2 times, then use 5% sodium sodium sodium sodium to disinfect 10 min, sterile water 3 times, use destruction Bacteria water absorbing paper suction surface spare;
[0060]Step (3):
[0061]The acquisition of the chestnut mature embryo: use a sterile anatomy to break and remove the skin, put the chestnut fruit to 75% alcohol soaked 2 min, flush with sterile water 3, then add 5 min sodium hypochlorite 5 min, soak After completing the fruit 5 times, then put it in a plate equipped with sterile suction p...
Embodiment 3
[0064]The difference from the first embodiment is that the concentration of IBA in the medium used in the culture medium used in step (7).
[0065]details as follows:
[0066]Step (7):
[0067]Rooting Culture: Under sterile conditions, use a sterile scissors to remove the base end of the seedling plant, inoculated to the root medium (WPM + 0.8mg / L IBA + 0.05mg / L Naa + sucrose 30g / L + agar 7.0 g / L, the pH was adjusted to 5.8 ~ 6.0 with 1 mol / l NaOH before the culture basis.) After 7 days, it was transferred to 1 / 2 wpm medium.
[0068]In this embodiment, the rooting rate of chestnut complex fuser seedlings is about 86%.
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