A method for fast breeding seedlings by tissue culture of mature embryos of fine chestnut

A technology for tissue culture and rapid propagation of seedlings, which is applied in horticultural methods, botany equipment and methods, plant regeneration, etc., can solve problems such as the inability to truly realize efficient and rapid propagation of chestnut seedlings, the impact of germination rate, and increase investment in tissue culture. Shorten the breeding cycle, ensure the germination rate, and solve the effect of rooting difficulties

Active Publication Date: 2021-04-16
HUANGGANG NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the patent "Excellent Chestnut Tissue Culture Rapid Propagation Method" (CN200410092406.3) invented by Sun Lixin and others and the patent "A Tissue Culture Technology of Chestnut" (CN201811447190.6) invented by Zhong Tianlu and others, The annual chestnut stem section and the budding stem section with strong growth activity were respectively selected as explants for tissue culture. Although chestnut seedlings were obtained in the end, the highly differentiated stem section needs to be dedifferentiated and redifferentiated to obtain a complete plant. , this treatment process will not only increase the investment in tissue culture, but also affect the germination rate, so that the efficient and rapid breeding of chestnut seedlings cannot be truly realized.

Method used

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  • A method for fast breeding seedlings by tissue culture of mature embryos of fine chestnut
  • A method for fast breeding seedlings by tissue culture of mature embryos of fine chestnut
  • A method for fast breeding seedlings by tissue culture of mature embryos of fine chestnut

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Embodiment 1

[0045]In this example, "花香" is used as an example, for explaining a method of culturing a fast-breeding seedlings according to the high-rated chestnut maturation of the present invention.

[0046]Includes the following steps:

[0047](1) The choice of high-rated chestnuts: Select the pepper, full, size, and no pathogenic "花香" chestnut, refrigerated in the refrigerator in the refrigerator in the refrigerator in the refrigerator;figure 1 The appearance of the selected chestnut;

[0048](2) Chestnut cleaning, disinfection: Choose fresh, healthy, free chestnut seeds rinse 20-30 min under the water (gently shaking or brush with brush), and eliminate floating seeds; In the net workbench, first use 75% alcohol soaked for 2min (soaking process needs to be continuously swayed to make the seed surface with disinfectant, the same), the sterile water is washed 2 times, then use 5% sodium sodium sodium sodium sodium 6min, sterile Water washing 3 times, use sterilized water absorbing paper to dry the surf...

Embodiment 2

[0056]The difference from the first embodiment is that the step (2), (3) the sterilization method of chestnut seeds and chestnut fruit.

[0057]details as follows:

[0058]Step (2):

[0059]Chestnut cleaning, disinfection: choose fresh, healthy, free chestnut seeds rinse 20-30 min under the water (gently shaking or brush with brush), and eliminate floating seeds; In this first, first use 75% alcohol soaked 2min (soaking process needs to make the seed surface with disinfectant), sterile water is washed 2 times, then use 5% sodium sodium sodium sodium to disinfect 10 min, sterile water 3 times, use destruction Bacteria water absorbing paper suction surface spare;

[0060]Step (3):

[0061]The acquisition of the chestnut mature embryo: use a sterile anatomy to break and remove the skin, put the chestnut fruit to 75% alcohol soaked 2 min, flush with sterile water 3, then add 5 min sodium hypochlorite 5 min, soak After completing the fruit 5 times, then put it in a plate equipped with sterile suction p...

Embodiment 3

[0064]The difference from the first embodiment is that the concentration of IBA in the medium used in the culture medium used in step (7).

[0065]details as follows:

[0066]Step (7):

[0067]Rooting Culture: Under sterile conditions, use a sterile scissors to remove the base end of the seedling plant, inoculated to the root medium (WPM + 0.8mg / L IBA + 0.05mg / L Naa + sucrose 30g / L + agar 7.0 g / L, the pH was adjusted to 5.8 ~ 6.0 with 1 mol / l NaOH before the culture basis.) After 7 days, it was transferred to 1 / 2 wpm medium.

[0068]In this embodiment, the rooting rate of chestnut complex fuser seedlings is about 86%.

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Abstract

The present invention provides a method for rapidly growing seedlings by tissue culture of mature mature embryos of Chinese chestnut, comprising the following steps: (1) selecting, cleaning and disinfecting Chinese chestnuts of high quality; (2) obtaining and inoculating mature Chinese chestnut embryos; (3) Cultivation of explants; (4) domestication and hardening of tissue culture seedlings. The method provided by the invention uses mature embryos as explants to carry out tissue culture, which greatly improves the germination and seedling rate of Chinese chestnut, shortens the breeding cycle, accelerates the breeding of improved varieties, improves the efficiency of Chinese chestnut germplasm innovation and new variety selection, and promotes The industrial development of the geographical indication product "Luotian Chestnut" of the General Administration of Quality Supervision, Inspection and Quarantine is of great significance.

Description

Technical field[0001]The present invention belongs to the field of fruit tree culture, and in particular, the present invention relates to a method of culturing a fast-breeding seedlings in high-rated chestnut mature embryo.Background technique[0002]Castanea Mollissima is a Castanea Moll, which is 7 kinds of bouncing plants, distributed in Asia, Europe, US, non-4 continents, is an important ecological economic forest tree species. In chestnut plants, Chinese chestnuts have an important position in quality, disease resistance and other utilization characteristics. In the area of ​​the province, the province is located in the eastern part of the province, between 30 ° 10 '~ 32 ° 30', and between 112 ° 40 '~ 117 ° 10', the Northern Tropical Sky Temperature Temple is very warm and humid climate. Suitable for chestnut growth, chestnut germplasm resources are very rich. Luotian, Macheng, Dafeng, Masheng County, Yingshan, in the area, accounting for more than 80% of the chestnut production...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 徐碧林陈岩岩郑永良高孟敏谢艳阳陶雷婷任玉燕万达黎爽
Owner HUANGGANG NORMAL UNIV
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