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Method for culturing rapid propagation seedlings in superior-species chestnut mature embryo tissue

A technology for tissue culture and rapid propagation of seedlings, applied in horticultural methods, botany equipment and methods, horticulture, etc., can solve problems such as the inability to truly realize efficient and rapid propagation of chestnut seedlings, increase investment in tissue culture, and affect the germination rate, etc., to achieve a large Application prospects and industrialization value, shortening breeding cycle, and fast breeding of improved varieties

Active Publication Date: 2019-11-05
HUANGGANG NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the patent "Excellent Chestnut Tissue Culture Rapid Propagation Method" (CN200410092406.3) invented by Sun Lixin and others and the patent "A Tissue Culture Technology of Chestnut" (CN201811447190.6) invented by Zhong Tianlu and others, The annual chestnut stem section and the budding stem section with strong growth activity were respectively selected as explants for tissue culture. Although chestnut seedlings were obtained in the end, the highly differentiated stem section needs to be dedifferentiated and redifferentiated to obtain a complete plant. , this treatment process will not only increase the investment in tissue culture, but also affect the germination rate, so that the efficient and rapid breeding of chestnut seedlings cannot be truly realized.

Method used

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  • Method for culturing rapid propagation seedlings in superior-species chestnut mature embryo tissue
  • Method for culturing rapid propagation seedlings in superior-species chestnut mature embryo tissue
  • Method for culturing rapid propagation seedlings in superior-species chestnut mature embryo tissue

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Embodiment 1

[0045] This embodiment takes "Osmanthus fragrans" Chinese chestnut as an example to illustrate the method for fast-growing seedlings of the mature embryo tissue culture of the excellent Chinese chestnut species described in the present invention.

[0046] Including the following steps:

[0047] (1) Selection of high-quality chestnuts: select Luotian "Osmanthus fragrans" chestnuts harvested in the current season that have been harvested in the season, which are plump, uniform in size, and free from diseases and insect pests, and refrigerated in a freezer at 0-4°C for later use; figure 1 Shows the appearance diagram of the selected Chinese chestnut;

[0048] (2) Cleaning and disinfection of chestnuts: select fresh, healthy, and non-cracked chestnut seeds and rinse them under tap water for 20-30 minutes (gently scrub with hands or brush gently), and eliminate floating seeds at the same time; In the clean workbench, first soak with 75% alcohol for 2 minutes (shake continuously duri...

Embodiment 2

[0056] The difference between the present embodiment and embodiment 1 is that the disinfection methods of steps (2), (3) chestnut seeds and chestnut fruits are different.

[0057] details as follows:

[0058] Step (2):

[0059] Cleaning and disinfection of chestnuts: select fresh, healthy, and non-cracked chestnut seeds and rinse them under tap water for 20-30 minutes (gently scrub with hands or brush gently), and eliminate floating seeds at the same time; In the seedlings, soak in 75% alcohol for 2 minutes (shake continuously during the soaking process to make the surface of the seeds fully contact with the disinfectant), wash with sterile water twice, then disinfect with 5% sodium hypochlorite for 10 minutes, wash with sterile water for 3 times, and use sterilized water. Blot the surface with the absorbent paper of bacteria for later use;

[0060] Step (3):

[0061] Acquisition of mature chestnut embryos: use a sterile scalpel to break open and remove the epidermis, soak ...

Embodiment 3

[0064] The difference between this embodiment and embodiment 1 is that: the concentration of IBA in the culture medium used in step (7) rooting culture is different.

[0065] details as follows:

[0066] Step (7):

[0067] Rooting culture: Under sterile conditions, use sterile scissors to cut off the base of the subculture seedlings, and inoculate into the rooting medium (WPM+0.8mg / L IBA+0.05mg / L NAA+sucrose 30g / L+agar 7.0g / L, before the medium is sterilized, adjust the pH to 5.8-6.0 with 1mol / L NaOH.), transfer to 1 / 2WPM medium after 7 days.

[0068] In the present embodiment, the rooting rate of chestnut subculture sprouts is about 86%.

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Abstract

The invention provides a method for culturing rapid propagation seedlings in superior-species chestnut mature embryo tissue. The method comprises the following steps of 1, selecting, cleaning and disinfecting superior-species chestnuts; 2, obtaining and inoculating chestnut mature embryos; 3, culturing an explant; 4, performing acclimation seedling hardening of tissue culture seedlings. Accordingto the method, mature embryos are adopted as explants for performing tissue culture, the germination planting percent of chestnuts is greatly increased, the breeding period is shortened, stock breeding is accelerated, the chestnut germ plasm innovation and new-variety breeding efficiency are improved, and the method is of a great significance in pushing the industrial development of the State Quality Inspection Administration geographic marking product 'Luotian chestnut'.

Description

technical field [0001] The invention belongs to the technical field of fruit tree cultivation, and in particular relates to a method for fast-growing seedlings by tissue culture of mature embryos of an excellent species of Chinese chestnut. Background technique [0002] Chestnut (Castanea mollissima) is a plant of the genus Castanea moll in the Fagaceae family. There are 7 species in the same genus. It is distributed in 4 continents, Asia, Europe, America, and Africa. It is an important ecological and economic forest tree species. Among Chestnut plants, Chinese Chestnut plays an important role in utilization characteristics such as quality and disease resistance. The Hubei section of the Dabie Mountains is located in the eastern part of the province, between 30°10′-32°30′ north latitude and 112°40′-117°10′ east longitude. It is suitable for the growth of chestnut, and the germplasm resources of chestnut are very rich. The four counties and cities of Luotian, Macheng, Dawu ...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 徐碧林陈岩岩郑永良高孟敏谢艳阳陶雷婷任玉燕万达黎爽
Owner HUANGGANG NORMAL UNIV
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