Method for culturing rapid propagation seedlings in superior-species chestnut mature embryo tissue

A technology for tissue culture and rapid propagation of seedlings, applied in horticultural methods, botany equipment and methods, horticulture, etc., can solve problems such as the inability to truly realize efficient and rapid propagation of chestnut seedlings, increase investment in tissue culture, and affect the germination rate, etc., to achieve a large Application prospects and industrialization value, shortening breeding cycle, and fast breeding of improved varieties

Active Publication Date: 2019-11-05
HUANGGANG NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the patent "Excellent Chestnut Tissue Culture Rapid Propagation Method" (CN200410092406.3) invented by Sun Lixin and others and the patent "A Tissue Culture Technology of Chestnut" (CN201811447190.6) invented by Zhong Tianlu and others, The annual chestnut stem section and the budding stem section with strong growth activity were respectively selected as explants for tissue c

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  • Method for culturing rapid propagation seedlings in superior-species chestnut mature embryo tissue
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  • Method for culturing rapid propagation seedlings in superior-species chestnut mature embryo tissue

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Example Embodiment

[0044] Example 1

[0045] This example takes the "Osmanthus fragrans" chestnut as an example to illustrate the method for rapid propagation of seedlings from mature embryo tissue culture of the superior species of chestnut in the present invention.

[0046] Including the following steps:

[0047] (1) Selection of high-quality chestnuts: select the "Osmanthus fragrans" chestnut harvested in the current season, which has been harvested in the season, is full, full, consistent in size, and free from pests and diseases, and refrigerates it in a refrigerator at 0~4℃ for later use; figure 1 Shows the appearance of the selected chestnut;

[0048] (2) Washing and disinfection of chestnut: Choose fresh, healthy, and unsplit chestnut seeds and rinse them under running water for 20-30 minutes (gently scrub by hand or brush gently), and at the same time eliminate floating seeds; In the clean bench, first soak with 75% alcohol for 2 minutes (during the soaking process, you need to keep shaking to ...

Example Embodiment

[0055] Example 2

[0056] The difference between this embodiment and the embodiment 1 lies in that the sterilization methods of the chestnut seeds and the chestnut fruit are different in steps (2) and (3).

[0057] details as follows:

[0058] Step (2):

[0059] Washing and disinfecting chestnuts: Choose fresh, healthy, and unsplit chestnut seeds and rinse them under running water for 20-30 minutes (gently scrub by hand or brush gently), and at the same time eliminate floating seeds; on the ultra-clean workbench Soak the seeds in 75% alcohol for 2 minutes (during the soaking process to keep the seeds in contact with the disinfectant), wash twice with sterile water, then sterilize with 5% sodium hypochlorite for 10 minutes, wash 3 times with sterile water, and use sterile water. Bacteria absorbent paper to dry the surface for later use;

[0060] Step (3):

[0061] Obtaining mature chestnut embryos: use a sterile scalpel to break open and remove the epidermis, soak the chestnut fruit in ...

Example Embodiment

[0063] Example 3

[0064] The difference between this embodiment and embodiment 1 lies in: the concentration of IBA in the medium used in step (7) rooting culture is different.

[0065] details as follows:

[0066] Step (7):

[0067] Rooting culture: under aseptic conditions, use sterile scissors to cut off the basal end of the subcultured seedlings, and inoculate to rooting medium (WPM+0.8mg / L IBA+0.05mg / L NAA+sucrose 30g / L+agar 7.0g / L, before the medium is sterilized, adjust the pH to 5.8-6.0 with 1mol / L NaOH.), transfer to 1 / 2WPM medium after 7 days.

[0068] In this example, the rooting rate of the successive sprouts of chestnut is about 86%.

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Abstract

The invention provides a method for culturing rapid propagation seedlings in superior-species chestnut mature embryo tissue. The method comprises the following steps of 1, selecting, cleaning and disinfecting superior-species chestnuts; 2, obtaining and inoculating chestnut mature embryos; 3, culturing an explant; 4, performing acclimation seedling hardening of tissue culture seedlings. Accordingto the method, mature embryos are adopted as explants for performing tissue culture, the germination planting percent of chestnuts is greatly increased, the breeding period is shortened, stock breeding is accelerated, the chestnut germ plasm innovation and new-variety breeding efficiency are improved, and the method is of a great significance in pushing the industrial development of the State Quality Inspection Administration geographic marking product 'Luotian chestnut'.

Description

technical field [0001] The invention belongs to the technical field of fruit tree cultivation, and in particular relates to a method for fast-growing seedlings by tissue culture of mature embryos of an excellent species of Chinese chestnut. Background technique [0002] Chestnut (Castanea mollissima) is a plant of the genus Castanea moll in the Fagaceae family. There are 7 species in the same genus. It is distributed in 4 continents, Asia, Europe, America, and Africa. It is an important ecological and economic forest tree species. Among Chestnut plants, Chinese Chestnut plays an important role in utilization characteristics such as quality and disease resistance. The Hubei section of the Dabie Mountains is located in the eastern part of the province, between 30°10′-32°30′ north latitude and 112°40′-117°10′ east longitude. It is suitable for the growth of chestnut, and the germplasm resources of chestnut are very rich. The four counties and cities of Luotian, Macheng, Dawu ...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 徐碧林陈岩岩郑永良高孟敏谢艳阳陶雷婷任玉燕万达黎爽
Owner HUANGGANG NORMAL UNIV
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