Method for strengthening and rooting tissue culture seedlings of transgenic peanuts

A technology of tissue culture seedlings and transgenics, which is applied in horticultural methods, botany equipment and methods, plant regeneration, etc., can solve the problems of inability to achieve multiplication and strong seedlings simultaneously, affect the survival rate of transplanting, and poor rooting quality. Significant benefit and scientific research value, improved transplant survival rate, vigorous growth effect

Active Publication Date: 2012-07-18
SHANDONG PEANUT RES INST
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

As far as peanut tissue culture is concerned, there is still no effective method for strong seedlings and rooting. The existing methods cannot simultaneously achieve the purpose of multiplication and strong seedlings, and the rooting rate is low and the quality of rooting is poor, which seriously affects the later stage of transplanting. Survival rate

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Transfer the clustered buds of 51 peanut Huayu 26 strains induced and differentiated to the strong seedling medium A and cultivate them for 20 days. When transferring, divide the buds with better growth into individual plants, and use a sterile scalpel to cut off the bottom and contact with the medium to form The dark brown part of the stem, the bottom stem end is immersed in the medium 2~3mm for cultivation, and some unformed buds are divided into several bud clusters for cultivation. The components of strong seedling medium A are MS medium with 1 mg / L cytokinin 6-BA (concentration in recovery and induction medium is 3 mg / L), pH=5.8. The medium is replaced once every 10 days, and the clustered Those that grew better in the buds were divided into individual plants again. After being cultured in medium A for strong seedlings, move the single transgenic seedlings with a growth of more than 1.5 cm to medium B for strong seedlings for 25 days, cut off the dark brown part fo...

Embodiment 2

[0029] Transfer the clustered buds of 47 peanut Huayu 17 strains induced and differentiated to the strong seedling medium A and cultivate them for 20 days. When transferring, divide the buds with better growth into individual plants, and cut off the bottom with a sterile scalpel to contact with the medium to form The dark brown part of the stem, the bottom stem end is immersed in the medium 2~3mm for cultivation, and some unformed buds are divided into several bud clusters for cultivation. The components of strong seedling medium A are MS medium with 1 mg / L cytokinin 6-BA (concentration in recovery and induction medium is 3 mg / L), pH=5.8. The medium is replaced once every 10 days, and the clustered Those that grew better in the buds were divided into individual plants again. After being cultured in medium A for strong seedlings, move the transgenic seedlings with a growth of more than 1.5 cm to medium B for strong seedlings for 20 days, and cut off the dark brown part formed b...

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Abstract

The invention provides a method for strengthening and rooting tissue culture seedlings of transgenic peanuts. The method has a good seedling strengthening effect and is high in rooting rate and rooting quality. The method comprises the following steps of: (1) transferring peanut tissue culture seedlings on which cluster buds are induced and differentiated into a seedling strengthening culture medium A, culturing for 20 days, and replacing the culture medium once every 10 days, wherein a component of the seedling strengthening culture medium A is a Murashige and Skoog (MS) culture medium, and used hormone is cytokinin 6-benzylaminopurine (6-BA); (2) dividing the tissue culture seedlings with the heights of more than 1.5cm into single plants, transferring into a seedling strengthening culture medium B, culturing for 20 to 25 days, and replacing the culture medium once every 10 days, wherein a component of the seedling strengthening culture medium B is the MS culture medium, and used hormone is cytokinin 6-BA at the concentration of 0.4mg/L and auxin naphthyl acetic acid (NAA) at the concentration of 0.1mg/L; and (3) transferring into a rooting culture medium when the seedlings with the heights of 2.5 to 4cm, and rooting, wherein a component of the rooting culture medium is a one/second MS culture medium, and used hormone is auxin indolebutyric acid (IBA) at the concentration of 3mg/L and auxin NAA at the concentration of 0.3mg/L.

Description

[0001] technical field [0002] The invention relates to a method for cultivating peanut seedlings, in particular to a method for growing seedlings and rooting of transgenic peanut tissue cultured seedlings. [0003] Background technique [0004] The use of tissue culture methods for peanut propagation has the following advantages: first, it can reproduce in large quantities in a short period of time, which is of great significance to the breeding of rare varieties and the current peanut transgenic work; second, it occupies a small space and is not limited by regions and seasons; in addition , the culture conditions are artificially controllable, and the conditions are uniform, which is convenient for stable cultivation and production. [0005] The rooting link is a key link in the whole peanut tissue culture process, and it undertakes the induction and differentiation link in the early stage and the transplanting link in the later stage. The rooting quality of tissue cult...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 陈明娜禹山林杨庆利潘丽娟王冕迟晓元杨珍陈娜王通和亚男
Owner SHANDONG PEANUT RES INST
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