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Dipixiao's propagation method

A technology for land disinfection, disinfection and sterilization, which is applied in horticultural methods, botany equipment and methods, plant regeneration, etc., to achieve the effects of simplifying cultivation procedures, facilitating the arrangement of production plans, and saving land resources

Active Publication Date: 2019-06-18
YUNNAN UNIV OF TRADITIONAL CHINESE MEDICINE
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to solve the defects of Dipixiao's existing breeding technology, and provide a method for obtaining high-quality and large-quantity Dipixiao asexual seedlings in a short period of time. This method lays a solid foundation for protecting Dipixiao wild resources and developing artificial planting. Technical basis, at the same time, the excellent traits can be fixed, and high-quality seedlings with consistent genotype background can be provided to meet the needs of artificial planting

Method used

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  • Dipixiao's propagation method
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Experimental program
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Effect test

Embodiment

[0029] 1. Acquisition of explants: Select healthy plants with good growth potential, no damage by diseases and insect pests, and no deformities, and get the 3-5 stem segments under the shoot tip, and cut them into about 2-3cm long with a scalpel.

[0030] 2. Clean the soil and dust on the surface of the budded stem section in step 1 with tap water, then rinse it under running water for 2 hours, put it on an ultra-clean workbench and disinfect it with 75% (volume ratio) alcohol for 60 seconds, and then use 0.1 %HgCl 2 (Mass ratio) Sterilize for 6 minutes, and finally rinse with sterile water for 8 times and place on sterile filter paper to blot the surface moisture. Shake the utensils well throughout the sanitization process.

[0031] 3. Simultaneous culture of callus induction, cluster bud formation and proliferation: take the stem segment with buds that has been sterilized in 2 steps as the material, cut it into 1.5-2.0cm long, and insert it into the following medium:

[00...

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Abstract

The invention provides a pararuellia delavayana reproduction method. The method comprises the steps that a disinfected and sterilized pararuellia delavayana explant is inoculated into different culture media successively for callus induction, bud production and reproduction synchronous culture. According to the pararuellia delavayana reproduction method, the common vitrification phenomenon produced during rapid asexual propagation is avoided, the cost is low, time is short, and the quality and survival rate are high.

Description

technical field [0001] The invention relates to the propagation technology of Chinese herbal medicine, in particular to a propagation method of dipixiao. Background technique [0002] Pararuellia delavayana is a perennial herb of the genus Pararuellia in the family Acanthaceae. It is also known as Dipijiao, incision medicine, and maggot medicine. It is a unique medicinal plant in my country. It is produced in southern Sichuan, north-central Yunnan and Guizhou. 750-3000m mountain grass slopes, under sparse forests. Dipixiao whole herb is used as a medicine to clear away heat and detoxify, dissipate blood stasis and reduce swelling, pull out toxin and promote granulation, dissipate blood stasis and regenerate, and kill insects. It is mainly used to treat pneumonia, tonsillitis, mumps, scrofula, abscess sores, fractures, etc. In Yunnan Xundian, Shilin, Maitreya and other native places, the local people call Dipixiao a "little miracle medicine", which is used for colds and fever...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 黄衡宇曹磊
Owner YUNNAN UNIV OF TRADITIONAL CHINESE MEDICINE
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