A New Method of Artificial Rapid Propagation of Arrowhead Mushroom

A technology of Shanci mushroom and a new method is applied in the field of artificial rapid propagation of Shanci mushroom, which can solve the problems of long ramet breeding cycle, slow breeding process, limited planting scale, etc. Effects of low resolution coefficients

Active Publication Date: 2021-05-07
YUNNAN UNIV OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The technical problem to be solved by the present invention is to provide a new method for rapid artificial propagation of Arthrobus chinensis to solve the problems of long reproductive cycle, low efficiency, slow breeding process and extremely limited planting scale in the artificial cultivation of Arthrowia chinensis

Method used

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  • A New Method of Artificial Rapid Propagation of Arrowhead Mushroom
  • A New Method of Artificial Rapid Propagation of Arrowhead Mushroom
  • A New Method of Artificial Rapid Propagation of Arrowhead Mushroom

Examples

Experimental program
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Effect test

Embodiment 1

[0070] A kind of new method of artificial fast multiplication of Shanzi mushroom, comprises the following steps:

[0071] 1. Acquisition of explants: select the plants of Arrowhead mushrooms with excellent phenotypes, and take their pseudobulbs as explants.

[0072] 2. Wash the surface soil of the pseudobulb in step (1) with tap water first, then soak it with 10% washing powder solution (mass ratio) for 10 minutes and then rinse it with running water for 30 minutes after slightly shaking and stirring, and place it on a super-clean workbench; 5% vitamin C Soak in (VC) solution (mass ratio) for 3 minutes, treat with 75% ethanol solution (volume ratio) for 13 seconds, then disinfect with 0.1% mercuric chloride solution (mass ratio) for 9 minutes, and finally rinse with sterile water 6 times, each time for 3 minutes. Shake the utensils well throughout the sanitization process.

[0073] 3. Acquisition of aseptic seedlings: the pseudobulbs sterilized and sterilized in step (2) are ...

Embodiment 2

[0089] A kind of new method of artificial fast multiplication of Shanzi mushroom, comprises the following steps:

[0090] 1. Acquisition of explants: select the plants of Arrowhead mushrooms with excellent phenotypes, and take their pseudobulbs as explants.

[0091] 2. Wash the surface soil of the pseudobulb in step (1) with tap water first, then soak it with 10% washing powder solution (mass ratio) for 10 minutes and then rinse it with running water for 30 minutes after slightly shaking and stirring, and place it on a super-clean workbench; 5% vitamin C Soak in (VC) solution (mass ratio) for 3 minutes, treat with 75% ethanol solution (volume ratio) for 15 seconds, then disinfect with 0.1% mercuric chloride solution (mass ratio) for 8 minutes, and finally rinse with sterile water 5 times, each time for 3 minutes. Shake the utensils well throughout the sanitization process.

[0092] 3. Acquisition of sterile seedlings: Cut the sterilized pseudobulbs of step (2) into 1.7 cm in ...

Embodiment 3

[0109] A kind of new method of artificial fast multiplication of Shanzi mushroom, comprises the following steps:

[0110] 1. Acquisition of explants: select the plants of Arthia chinensis with excellent phenotypes, and take the pseudobulbs thereof as explants.

[0111] 2. Wash the surface soil of the pseudobulb in step (1) with tap water first, then soak it with 10% washing powder solution (mass ratio) for 10 minutes and then rinse it with running water for 30 minutes after slightly shaking and stirring, and place it on a super-clean workbench; 5% vitamin C Soak in (VC) solution (mass ratio) for 3 minutes, treat with 75% ethanol solution (volume ratio) for 14 seconds, then disinfect with 0.1% mercuric chloride solution (mass ratio) for 10 minutes, and finally rinse with sterile water 5 times, each time for 3 minutes. Shake the utensils well throughout the sanitization process.

[0112] 3. Acquisition of sterile seedlings: Cut the pseudobulbs sterilized and sterilized by step ...

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Abstract

The present invention discloses a new method for artificial fast propagation of Shanci mushroom, comprising the following steps: (1) obtaining explants; (2) washing the surface soil of the pseudobulbs in step 1 with tap water, and then soaking them with washing powder solution Rinse with running water, then process with ethanol solution, and rinse after disinfection; (3) obtain sterile seedlings; (4) take the sprouts grown from the bud spots in step 3 and transfer them to the A medium in step 3 Proliferation culture is carried out inside, and the seedlings carrying callus are cut off, and continue to be transferred into B medium to cultivate; (5) the single seedling with rice grain size pseudobulb after subculture in step 4 is inoculated in C medium (6) seedling refining and transplanting. The invention optimizes and adjusts the rapid propagation in vitro of S. chinensis, simplifies the artificial rapid propagation process, greatly improves the propagation efficiency, has low cost and short time, not only improves the survival rate of the test-tube seedlings, but also greatly shortens the seedling raising period. .

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a new method for artificial rapid propagation of Arrowhead mushroom. Background technique [0002] Shanzi mushroom [Cremastra appendiculata (D.Don) Makino], also known as Rhododendron orchid, is a perennial herb of Orchidaceae (Orchidaceae) Rhododendron orchid (Cremastra)), which grows in ditch edge wetlands or hillside forest shade wetlands, at an altitude of 500- 2900m, usually produced in Gongshan, Fengqing, Yuxi and other places in Yunnan; the distribution range includes Tibet, Guizhou, southern Shaanxi and southern Shanxi in China, and also in Nepal, Bhutan, India, Vietnam, Thailand and Japan abroad. distributed. [0003] Arrowhead mushroom is used as medicine with pseudobulb, which is one of the original plants of Arrowhead mushroom. It tastes sweet, slightly pungent, and slightly poisonous. Efficacy, internal use can resist liver cancer, breast cancer, uterine ca...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 黄衡宇徐福荣
Owner YUNNAN UNIV OF TRADITIONAL CHINESE MEDICINE
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