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Artificial efficient propagation method of aristolochia tuberosa

A kind of back snake growing and artificial technology, applied in the field of Chinese herbal medicine reproduction, can solve the problems of limited germination season, small biomass, slow growth of seedlings, etc., and achieve the effect of simplifying the cultivation procedure, easy to standardize, and fast to multiply.

Active Publication Date: 2021-01-05
YUNNAN UNIV OF TRADITIONAL CHINESE MEDICINE
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] In view of the above problems, the present invention provides a kind of artificial high-efficiency propagation method of back snake, to solve the low germination rate of back snake seeds, slow growth of seedlings and The biomass is small, the variation of offspring is large, and it is limited by the germination season, it is difficult to carry out large-scale and standardized planting, and it is difficult to popularize high-efficiency varieties on a large scale

Method used

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  • Artificial efficient propagation method of aristolochia tuberosa

Examples

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Effect test

Embodiment 1

[0049] Such as figure 1 , figure 2 and image 3 Shown, a kind of artificial high-efficiency breeding method of back snake life, comprises the following steps:

[0050] 1. Acquisition of explants: Select healthy plants with good growth potential, no damage by diseases and insect pests, and no deformities, and get the stem section under the tip of the shoot, including the 3rd to 5th sections, and cut it into about 2.0cm long stem sections with a scalpel.

[0051] 2. After washing the segmented stem section in step 1 with running water, soak it with a mass ratio of 15% washing powder solution for 10 minutes, rinse it with running water for 1 hour and place it in an ultra-clean workbench; soak it with a volume ratio of 75% ethanol solution for 10-15 seconds, Then sterilized with 0.1% mercuric chloride aqueous solution for 8 minutes, and finally rinsed with sterile water for 3 times, each time not less than 3 minutes, and placed on filter paper to absorb the residual sterile wat...

Embodiment 2

[0067] Such as figure 1 , figure 2 and image 3 Shown, a kind of artificial high-efficiency breeding method of back snake life, comprises the following steps:

[0068] 1. Acquisition of explants: Select a strong plant with good growth potential, no damage by diseases and insect pests, and no deformity, and get the stem section under the tip of the shoot, including the second to third sections, and cut it into a long stem section of about 1.7cm with a scalpel.

[0069] 2. After washing the segmented stem section in step 1 with running water, soak it with a mass ratio of 15% washing powder solution for 10 minutes, rinse it with running water for 1 hour and place it in an ultra-clean workbench; soak it with a volume ratio of 75% ethanol solution for 10-15 seconds, Then sterilized with 0.1% mercuric chloride aqueous solution for 8 minutes, and finally rinsed with sterile water for 3 times, each time not less than 3 minutes, and placed on filter paper to absorb the residual ster...

Embodiment 3

[0085] Such as figure 1 , figure 2 and image 3 Shown, a kind of artificial high-efficiency breeding method of back snake life, comprises the following steps:

[0086] 1. Acquisition of explants: Select a strong plant with good growth potential, no damage by diseases and insect pests, and no deformity, and get the stem section under the tip of the shoot, including 2-3 nodes, and cut it into a long stem section of about 1.5cm with a scalpel.

[0087] 2. After washing the segmented stem section in step 1 with running water, soak it with a mass ratio of 15% washing powder solution for 10 minutes, rinse it with running water for 1 hour and place it in an ultra-clean workbench; soak it with a volume ratio of 75% ethanol solution for 10-15 seconds, Then sterilized with 0.1% mercuric chloride aqueous solution for 8 minutes, and finally rinsed with sterile water for 3 times, each time not less than 3 minutes, and placed on filter paper to absorb the residual sterile water. Shake t...

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Abstract

The invention discloses an artificial efficient propagation method of aristolochia tuberosa. The method comprises the following steps of inoculating a disinfected and sterilized explant with a bud stem segments of the aristolochia tuberosa into a culture medium A for culture, starting propagation culture under the conditions that the illuminance is 1500-2000lx, the illumination time is 10h / d and the temperature is controlled to be 22 + / -1 DEG C, and obtaining a propagation coefficient of 3.57 after 35 days. A material is shorn into the stem segments containing 2-3 joints, the stem segments areinoculated into a culture medium B to be cultured, a clustered cluster system with base adventitious cluster buds and upper axillary buds growing is formed after 45 days, and the propagation coefficient reaches 10.0 or above. When the height of proliferated cluster buds is 3-4cm, single seedlings are inoculated into the culture medium B to be cultured for 45d to obtain rooted seedlings with strong seedlings and thick roots, and then the rooted seedlings are transplanted. The method is low in cost, short in period and high in test-tube plantlet quality and survival rate, provides technical support for protecting wild resources and breeding high-quality plantlets, and can be used for producing excellent plantlets with consistent genotype backgrounds in a short time.

Description

technical field [0001] The invention relates to the technical field of propagation of Chinese herbal medicines, in particular to an artificial high-efficiency propagation method for back snakes. Background technique [0002] Back Snake (Aristolochia tuberosa C.F.Liang et S.M.Hwang) is a perennial herbaceous vine of Aristolochiaceae (Aristolochiaceae) Aristolochia (Aristolochia), also known as poisonous snake medicine, avoiding snakes, cinnabar lotus, etc. The plants are mainly distributed in provinces such as Guangxi, Yunnan, Guizhou and Sichuan, and grow on limestone mountains at an altitude of 150-1600m or in bushes on both sides of ravines. The root tuber of back snake is used as medicine. It tastes bitter, pungent, cold in nature, and slightly poisonous. , hematemesis, and snakebite have better curative effect. [0003] With the rapid development of the big health industry, Back Snake Health is no longer limited to folk Chinese medicine applications. Modern medicine a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/008Y02A40/22
Inventor 黄衡宇张爱丽徐福荣席银凯邹梅
Owner YUNNAN UNIV OF TRADITIONAL CHINESE MEDICINE
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