A tidal bioreactor and method for cultivating lily seed balls

A bioreactor, lily seed ball technology, applied in the fields of botanical equipment and methods, horticultural methods, applications, etc., can solve the problems of high material and labor costs, complex production procedures, and difficulty in large-scale production, saving manpower and material resources. , Improve economic efficiency, the effect of seed ball stability

Active Publication Date: 2016-01-20
FLOWER RES INST OF YUNNAN ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In order to overcome the problems of low efficiency, high material and labor costs, relatively complicated production procedures, and difficulty in large-scale production in the existing lily seed bulb propagation technology

Method used

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  • A tidal bioreactor and method for cultivating lily seed balls
  • A tidal bioreactor and method for cultivating lily seed balls
  • A tidal bioreactor and method for cultivating lily seed balls

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0057] like Figure 1~3 As shown, a tidal bioreactor includes a nutrient solution storage container 2, a culture container 1, an air pump 3, a timer 4, an air filter membrane 5, a water saving valve 6 and a liquid addition filter 7, and the nutrient solution storage container 2 is connected to the culture container 1 through a conduit equipped with a water-saving valve 6, and the nutrient solution storage container 2 and the culture container 1 are respectively connected to the corresponding air pump 3 through a conduit equipped with an air filter membrane 5, and the The aspirating pump 3 is connected with the timer 4, and the timer 4 controls whether the aspirating pump 3 operates; the culture container 1 is provided with a metal gauze 8 parallel to the bottom surface, and the nutrient solution storage container and the aspirating pump The conduit between is provided with liquid-adding filter 7.

[0058] When need soaking culture, add culture medium from liquid-adding filter...

Embodiment 2

[0089] The tidal bioreactor of this embodiment is the same as that of Embodiment 1.

[0090] The tidal bioreactor of the present embodiment cultivates the method for lily bulb, comprises the steps:

[0091] A. Select a healthy growing lily ball with a diameter of 16-18cm, peel off the outer layer and the scales with spots and wormholes, separate the scales of the lily ball, wash it with washing powder water first, and then rinse it with clean water. After wiping the material twice with 75% alcohol in an ultra-clean environment, put it into a mercuric chloride solution with a mass concentration of 0.1% for disinfection for 15 minutes, then put it in the mixed solution for disinfection for 15 minutes; then rinse it with sterile water 3 times, 1 min each time, the lily explants were obtained, and the mixed solution was that every 100ml of mass concentration was 2% sodium hypochlorite solution, and 5 drops of Tween-20 were added dropwise to obtain the lily explants. Insert the im...

Embodiment 3

[0122] The tidal bioreactor of this embodiment is the same as that of Embodiment 1.

[0123] The tidal bioreactor of the present embodiment cultivates the method for lily bulb, comprises the steps:

[0124] A. Select a healthy growing lily ball with a diameter of 16-18cm, peel off the outer layer and the scales with spots and wormholes, separate the scales of the lily ball, wash it with washing powder water first, and then rinse it with clean water. After wiping the material twice with 75% alcohol in an ultra-clean environment, put it into a mercuric chloride solution with a mass concentration of 0.1% for disinfection for 15 minutes, then put it in the mixed solution for disinfection for 15 minutes; then rinse it with sterile water 3 times, 1 min each time, the lily explants were obtained, and the mixed solution was that every 100ml of mass concentration was 2% sodium hypochlorite solution, and 5 drops of Tween-20 were added dropwise to obtain the lily explants. Insert the im...

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Abstract

The present invention innovatively utilizes tidal bioreactors to rapidly propagate lily transplanted seed balls, by cultivating and growing lily seed balls with a diameter of 0.2 to 0.4 cm in conventional solid propagation medium as explants and inserting them into tidal bioreactors. In the bioreactor, the liquid medium formula was replaced during the 50-day cultivation process, and the pollution was controlled by adding drugs, and the seed balls were gradually expanded and rooted. After the seed balls were released from the bottle, the lily seed balls were vernalized, transplanted, Planting methods are managed. The present invention can simplify the tissue culture procedure by gradually carrying out seed ball expansion and rooting culture, greatly reduce the labor cost in conventional solid tissue culture, and can effectively control pollution and the generation of mutant strains, shorten the lily breeding cycle, and save The cost of production can quickly fix and maintain the excellent characters of new lily varieties, and solve the problems of slow breeding speed, mixed provenance and unstable quality of seed balls produced by conventional lily breeding systems.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and in particular relates to a tidal bioreactor and a method for cultivating lily balls. Background technique [0002] Lily is a perennial herbaceous bulb plant of the Liliaceae Lilium genus. It is a world-renowned ornamental flower. In recent years, with the development of my country's economy and the improvement of people's living standards, lily has become an important high-grade cut flower in my country's flower market. Widely used in garden greening, home decoration, festival flowers, medicinal food and other fields. With the continuous expansion of the lily demand market, fresh cut flower lilies, edible lilies and garden greening lily seed balls are widely planted in southwest, northwest, northeast and other regions of my country. The safety and rapid production and reproduction of lily seed bulbs as a provenance have also attracted more and more attention from the industry. ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 贾文杰蒋亚莲崔光芬赵培飞王祥宁段青马璐琳杜文文王继华
Owner FLOWER RES INST OF YUNNAN ACAD OF AGRI SCI
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