34 results about "Solid tissue" patented technology

Apparatuses and methods for recovering solid tissue and dislodged cells (“D-cells”) from a biopsy are disclosed herein. In an embodiment, a biopsy container apparatus for recovering solid tissue and D-cells from a biopsy includes a receiving container and a sieve container. The receiving container has a receiving chamber with an upper opening and a watertight bottom surface. The sieve container is configured to be located at least partially within the receiving chamber and removed from the receiving chamber through the upper opening. The sieve container has a bottom sieve surface including a plurality of apertures configured to pass the D-cells from the biopsy but not the solid tissue from the biopsy into the receiving chamber after the solid tissue and the D-cells from the biopsy are placed into the sieve container.

Apparatuses and methods for recovering solid tissue and dislodged cells (“D-cells”) from a biopsy are disclosed herein. In an embodiment, a biopsy container apparatus for recovering solid tissue and D-cells from a biopsy includes a buffer container, a sieve container and a sample collection container. The buffer container includes a buffer chamber. The sieve container is located least partially within the buffer container and includes a sieve surface configured to pass the D-cells from the biopsy but not the solid tissue from the biopsy into the buffer chamber. The sample collection container is removably attached to the buffer container and includes a reagent chamber.

The utility model relates to the technical field of medical instruments, and discloses a novel drainage apparatus for tumor patients, which comprises a main body mechanism and a fixing mechanism, the fixing mechanism is positioned on the outer side of the main body mechanism, the main body mechanism comprises a drainage apparatus body and a drainage tube, the drainage tube is fixedly mounted at the right end of the drainage apparatus body, and the drainage tube is connected with the fixing mechanism. The main body mechanism further comprises a filter screen, a fixing plate, a micro motor, a connecting shaft and a crushing piece. According to the novel drainage apparatus for the tumor patient, by installing the main body mechanism, solid tissues and blood clots in drainage effusion can be filtered through the design of a filter screen when the drainage apparatus body is used for conducting drainage operation on the tumor patient; and in cooperation with the design of a micro motor and a crushing piece, solid tissues and blood clots attached to the filter screen can be crushed, the situation that the drainage tube is blocked due to accumulation of the solid tissues and the blood clots is avoided, normal drainage operation of the drainage device body is guaranteed, and the protection performance of the drainage device body is improved.

The invention relates to the technical field of image processing, and discloses a cervical cancer pathological image analysis method guided by a two-stage generative adversarial network, which comprises the following steps: collecting a real pathological image of cervical cancer and labeling the real pathological image with a real label; constructing a cervical cancer pathology data generation network based on the two-stage generative adversarial network, wherein the cervical cancer pathology data generation network comprises a pathology image generation network and a pathology image label generation network; the cervical cancer pathology data generation network receives a real pathology image of the cervical cancer, marks a real label on the real pathology image, and outputs a generated pathology image and a corresponding generated label for joint training of a downstream segmentation network; and segmenting the cervical cancer pathological image by using the trained downstream segmentation network, and identifying cancerous tissues, solid tissues and background parts in the cervical cancer pathological image. By generating the pathological image data, the efficiency and accuracy of cervical cancer pathological image analysis are improved.

Apparatuses and methods for recovering solid tissue and dislodged cells (“D-cells”) from a biopsy are disclosed herein. In an embodiment, a biopsy container apparatus includes a sample collection container, a basket sieve, and a buffer container. The sample collection container includes a reagent chamber. The basket sieve is configured for removable attachment at least partially within the sample collection container and includes a sieve surface configured to pass the D-cells from the biopsy but not the solid tissue from the biopsy. The buffer container is configured for removable attachment to the sample collection container and includes a buffer chamber.

Apparatuses and methods for recovering solid tissue and dislodged cells ("D-cells") from a biopsy are disclosed herein. In an embodiment, a biopsy container apparatus for recovering solid tissue and D-cells from a biopsy includes a receiving container and a sieve container. The receiving container has a receiving chamber with an upper opening and a watertight bottom surface. The sieve container is configured to be located at least partially within the receiving chamber and removed from the receiving chamber through the upper opening. The sieve container has a bottom sieve surface including a plurality of apertures configured to pass the D-cells from the biopsy but not the solid tissue from the biopsy into the receiving chamber after the solid tissue and the D-cells from the biopsy are placed into the sieve container.

The application provides a method for separating nucleic acid binding proteins in solid tissue cells, and the method comprises the following steps: step S1, preparing solid tissue into a cell suspension 1; step S2, cross-linking the cell suspension 1 by using a cross-linking agent to form a cell suspension 2 with nucleic acid-NABPs complexes; step S3, lysing red blood cells in the cell suspension 2 of step S2 to remove high-abundance protein interference; step S4, after the cells treated in step S3 are lysed by using an organic reagent, the nucleic acid-NABPs complexes in the solid tissue cells are selectively enriched by using affinity chromatography; and step S5, after the nucleic acid-NABPs complexes obtained in step S4 are de-cross-linked, nucleic acid binding proteins (NABPs) in the solid tissue cells are obtained.

The present invention provides a gastrointestinal fluid decompressor, comprising a collection box, a suction assembly, wherein the suction assembly is mounted on the upper portion of the collection box; a filter screen connected to the side wall of the inner cavity of the collection box, and used to filter and process the gastrointestinal fluid; and a crushing mechanism, wherein the crushing mechanism comprises a crushing mesh cylinder, wherein two symmetrically distributed crushing mesh cylinders are connected to the bottom of the filter screen, a crushing assembly is disposed inside the crushing mesh cylinder, and a lifting assembly is disposed outside the crushing assembly. The present invention utilizes the filter screen to filter the gastrointestinal fluid, utilizes the crushing assembly to crush solid tissue in the filtered gastrointestinal fluid, and utilizes the lifting assembly to lift the crushed solid tissue filtered by the crushing mesh cylinder and send it into the crushing assembly for further crushing, and repeats the process multiple times until all solid tissue is crushed, thereby preventing larger solid tissue from clogging the discharge port of the collection box or other processing equipment, and facilitating subsequent processing of the gastrointestinal fluid.

Apparatuses and methods for recovering solid tissue and dislodged cells (“D-cells”) from a biopsy are disclosed herein. In an embodiment, a biopsy container apparatus includes a sample collection container, a basket sieve, and a buffer container. The sample collection container includes a reagent chamber. The basket sieve is configured for removable attachment at least partially within the sample collection container and includes a sieve surface configured to pass the D-cells from the biopsy but not the solid tissue from the biopsy. The buffer container is configured for removable attachment to the sample collection container and includes a buffer chamber.

The application discloses a kind of negative pressure suction gun, manual negative pressure suction method, it is related to biomedical engineering technical field, the gun handle of negative pressure suction gun is reciprocated and opened and closed to drive suction pumping section to reciprocate in negative pressure sedimentation cavity Negative pressure suction and extrusion pumping, negative pressure sedimentation cavity can pass through liquid suction pipe one-way liquid suction when negative pressure suction, negative pressure sedimentation cavity can be one-way liquid delivery to the liquid storage part when extrusion pumping, so as to be used for suction liquid and soft solid tissue in liquid by setting negative pressure sedimentation cavity, then by adjusting increase negative pressure suction force, the viscosity of solid tissue that can be sucked is also greater, so that the negative pressure suction gun of the application can not only be used for endometrial gynecological field liquid suction and solid tissue sampling, but also can be used for closed narrow space tumor biopsy, brain tissue and other solid-liquid components suction, during suction process, by rotating liquid suction pipe, the inlet slope of liquid suction pipe is realized 360 degree rotation, to avoid suction dead angle.

The utility model discloses a primary cell extraction device for experiments, and relates to the technical field of experimental apparatuses. The device comprises a grinding cylinder, a buckling cover body and a stirring mechanism, the buckling cover body is arranged on the surface of an opening of the grinding cylinder and is in buckling connection with the surface of the opening of the grinding cylinder to form a sealing structure, the stirring mechanism comprises a driving motor, a matched bevel gear and a linkage rod, and the driving motor is installed on one side of the surface of the buckling cover body. Through the arrangement of the grinding cylinder, the buckling cover body and the stirring mechanism, the driving motor is started, the driving bevel gear drives the linkage bevel gear and the matching bevel gear to move in opposite rotating directions, efficient cutting is carried out in the grinding cylinder through the stirring blade, and rapid smashing of solid tissue is achieved; meanwhile, a plurality of groups of grinding rods are driven by the grinding frame to sufficiently grind the tissue blocks, and the ground tissue is diluted and discharged by utilizing flushing fluid after grinding, so that a cell solution is collected, cells are more sufficiently digested, and the collection rate of the cells is improved.

The utility model discloses a crushing and separating device for plant extraction, which belongs to the technical field of plant extraction and comprises an outer barrel body and a feed port arranged in the middle of the upper end of the outer barrel body, a separating barrel is mounted in the middle of the inner side of the outer barrel body, and a first discharge pipe is arranged at the lower end of the separating barrel. A second discharging pipe is installed at the lower end of an area defined by the separation barrel and the outer barrel body, a servo motor is installed on the side edge of the upper end of the outer barrel body, a power gear is installed at the output end of the servo motor, a linkage gear ring is arranged on the side edge of the power gear, and the linkage gear ring is fixed to the separation barrel; and a movable frame is mounted on the edge of the inner side of the separation barrel. According to the crushing and separating device for plant extraction, plants can be effectively crushed, meanwhile, when crushed plant tissue fluid is separated, plant solid tissue attached to the net barrel can be cleaned, and holes in the net barrel are prevented from being blocked by the solid tissue.

The invention provides a double-shoulder osteotome capable of realizing accurate positioning and controllable direction in an operation, which comprises an osteotome body provided with a blade for cutting solid tissues; the positioning bracket is connected with the osteotome body and is used for indicating the position and posture of the double-shoulder osteotome; the cutting edge positioning module and the double-shoulder bone knife are designed in a split mode, and the cutting edge positioning module is used for calibrating the cutting edge; the connection between the positioning support and the osteotome body has a movable state that the positioning support rotates along the osteotome body and a locking state that the positioning support is locked at a certain rotation angle. According to the osteotome, through the accurate mechanical structure and the auxiliary system, accurate control over the position and direction of the osteotome in the operation process is achieved, and the accuracy and safety of the operation are improved.

The present invention relates to the technical field of enzymatic detection. Disclosed in the present invention are a method and kit for determining the concentration of D-2-hydroxyglutaric acid. The determination method is not used for the diagnosis and treatment of diseases. D-2-hydroxyglutaric acid in a solid tissue section is detected by means of using a system comprising an FAD-dependent D-2-hydroxyglutarate dehydrogenase, resazurin and a PBS buffer as a working solution. During the preparation of a standard curve, D-2-hydroxyglutaric acid with different concentrations is added to an aqueous agarose solution to prepare standard sections. According to the method and kit for determining the concentration of D-2-hydroxyglutaric acid of the present invention, the concentration of D-2-hydroxyglutaric acid in a tissue sample can be detected after a simple treatment of the tissue sample, the detection result is consistent with that of a mass spectrometry detection method, and the accuracy is high. Detecting standard sections and preparing a sample section require 20 minutes, detecting the sample section requires about 3 minutes, and calculating the concentration of D-2-HG requires about 2 minutes, that is, only less than 5 minutes are required after the standard curve and sample section are prepared, which greatly shortens the detection time and reduces the detection cost.

The present invention relates to the field of image processing technology, and discloses a cervical cancer pathology image analysis method guided by a two-stage generative adversarial network, comprising the following steps: collecting real pathology images of cervical cancer and marking them with real labels; constructing a cervical cancer pathology data generation network based on the two-stage generative adversarial network, including a pathology image generation network and a pathology image label generation network; the cervical cancer pathology data generation network receives real pathology images of cervical cancer and marks them with real labels, outputs generated pathology images and corresponding generated labels, and is used to jointly train a downstream segmentation network; and uses the trained downstream segmentation network to segment the cervical cancer pathology image to identify cancerous tissue, solid tissue and background parts. The present invention improves the efficiency and accuracy of cervical cancer pathology image analysis by generating pathology image data.

Disclosed herein are devices and methods for recovering solid tissue and exfoliated cells ("D-cells") from biopsy. In an embodiment, a biopsy container device includes a sample collection container, a basket screen, and a buffer container. The sample collection container includes a reagent chamber. The basket screen is configured for at least partially removable attachment within the sample collection container, and the basket screen includes a screen surface configured to permit passage of exfoliated cells from the biopsy but not permit passage of solid tissue from the biopsy. The buffer container is configured for removable attachment to a sample collection container, and the buffer container includes a buffer chamber.

The invention discloses a laparoscope system, comprising a control cabinet, a display is arranged on the surface of the control cabinet, the control cabinet is connected to a lens body through a line, a mirror body is arranged on one side of the lens body, an auxiliary device is arranged on the surface of the mirror body, the auxiliary device comprises a mounting ring and an ultrasonic generator, the mounting ring is fixedly connected to the surface of the mirror body, the mounting ring is composed of a circular ring and square blocks on both sides, the ultrasonic generator is arranged on one side of the mirror body, both sides of the ultrasonic generator are fixedly connected with plug blocks, the cross section of the plug blocks is L-shaped, and the surface of the mounting ring is provided with square holes. By arranging the auxiliary device, it is convenient for the auxiliary equipment to perform surgery, and normal tissue and tumor tissue, adipose tissue and gland, cystic and solid tissue, fiber bundles, fascia, blood vessels and lymphatic vessels can be effectively distinguished, the phenomenon of causing damage to some normal tissues is reduced, the situation that the operation efficiency of the equipment is low and affects the operation is reduced, and the overall practicality of the equipment is improved.

The invention provides a multiple myeloma detection system based on single cell in-situ sequencing. The CosMxSMI technology is applied to hematologic tumors for the first time, limitation of solid tissues is broken through, and synchronous analysis of cell types, RNA and protein in-situ expression in a bone marrow microenvironment is achieved. A single-cell suspension sample smaller than 1 mL is used, common protein targets are subjected to flow analysis based on RNA FISH and MM of an MM-related customized panel, and information such as bone marrow or peripheral blood single-cell-level cell types, proportions, RNA and protein expression profiles and the like can be obtained in situ on a slide microarray at the same time. Traditional FISH, flow cytometry and GEP step-by-step detection are replaced, manpower of the pathology department and a blood cell chamber is saved, the sample collection amount, loss and operation errors are reduced, and the clinical report time is shortened by 80%.

The invention relates to the technical field of tissue preservation solutions, and provides a solid tissue flow detection sample preservation solution and a preparation method thereof, and the preservation solution comprises N-acetylcysteine, melatonin, lactobionic acid and an HBSS buffer solution. NAC, melatonin and lactobionic acid jointly act on an antioxidant pathway, ROS is removed, accumulation of lipid peroxidation products (MDA) is reduced, the mitochondrial function is protected, and the cell survival rate is increased. The melatonin and lactobionic acid act together to inhibit ferroptosis paths, maintain the health state of cells and improve the cell survival rate and the stability of antigen epitopes. All the components and a stable environment provided by HBSS act together to maintain the high activity of cells and the stability of antigen epitopes.

The invention belongs to the technical field of biomedicine, and particularly relates to a rat articular cavity sampling method. According to the method, the articular cavity sampling method is optimized, the optimized articular cavity is a complete articular cavity which contains few sclerotin and is free of subpatella ligaments and redundant periosteum and muscles, solid tissue interference is reduced to the maximum extent on the basis that the complete cavity is reserved, and the method has the advantages of being high in drug detection rate, high in drug concentration and good in data uniformity; the invention provides a material drawing scheme for subsequent preclinical test design, and has important scientific significance and application value for developing new medicines for treating joint diseases such as osteoarthritis and the like.

The utility model discloses a drainage bag used in an ICU (Intensive Care Unit) operation, particularly relates to the technical field of medical equipment, and comprises a drainage bag body, a liquid outlet communicated and connected with the bottom of the drainage bag body and a liquid inlet catheter communicated and connected with the top of the drainage bag body, and the drainage end of the liquid inlet catheter is communicated and connected with a connector. The cleaning device has the advantages that the sealing ball is jacked open under the action of pressure by pressing the perfusion device, cleaning liquid enters the inner side of the flushing pipe through the perfusion head, and then tissue solids attached to the filter plate are cleaned through the flushing head; the cleaned solid tissues are discharged through the liquid outlet end and are received by a container, and after the perfusion apparatus is pulled out, the sealing ball is reset by utilizing the elastic acting force of the spring, so that the drainage bag body can be effectively prevented from being blocked in the human body tissue fluid drainage process, medical personnel do not need to replace the drainage bag body, and the operation is simple and convenient. And precious time is gained for rescuing the critically ill patients.

The present invention relates to a method for isolating and culturing tissue-resident uPAR+ / Nestin+ stem cells, and a use thereof, and provides a method for isolating, on the basis of uPAR-plasmin activity, tissue-resident stem cells present in solid tissues, wherein the uPAR-plasmin activity of stem cells has been suggested to have a close relationship with stem cell growth, migration ability, physiological activity and differentiation ability, and thus can be used in a method for isolating high-potency stem cells, the uPAR+ stem cells isolated from solid tissues are applicable to the production of cell therapeutic agents, tissue engineering therapeutic agents, and novel biopharmaceuticals using secretions including exosomes, and thus have high industrial applicability, and the present invention is applicable to fundamental cell biology and molecular biology research and novel drug development research that relate to the cell division, migration, growth, and differentiation of tissue-resident stem cells.

Apparatuses and methods for recovering solid tissue and dislodged cells (“D-cells”) from a biopsy are disclosed herein. In an embodiment, a biopsy container apparatus for recovering solid tissue and D-cells from a biopsy includes a buffer container, a sieve container and a sample collection container. The buffer container includes a buffer chamber. The sieve container is located least partially within the buffer container and includes a sieve surface configured to pass the D-cells from the biopsy but not the solid tissue from the biopsy into the buffer chamber. The sample collection container is removably attached to the buffer container and includes a reagent chamber.

The invention relates to the technical field of cell targeted therapy, in particular to a construction method and application of a cell robot for enhancing cell therapy targeting and entity penetration. The invention relates to a construction method of a cell robot for enhancing cell therapy targeting and entity penetration. The construction method comprises the following steps: coupling magnetic microfilaments on the surfaces of cells; the magnetic microfilament comprises a microfilament and magnetic particles attached to the microfilament; and the microfilaments are made of polylactic acid-glycolic acid copolymer. According to the technical scheme, the technical problem that in the prior art, chimeric antigen receptor modified macrophages with high targeting and penetrating capacity are lacked can be solved, and the technical problems that in the prior art, chimeric antigen receptor cells generally face infiltration of solid tissues and infiltration is not ideal are further solved. The engineering cell design combining the bionic sperm model and magnetic driving breaks through the bottleneck of the traditional cell therapy in solid tissue delivery, provides a brand new technical thought and method for wider cell therapy optimization, and has ideal application and popularization values.

The present invention discloses a steel shell component with a gradient distribution of microstructure and mechanical properties and a forming method. The steel blank base material is preformed into a steel blank with a semi-solid structure, placed in a preheated concave die, and the punch is driven to press the steel blank down to achieve thixotropic plastic deformation composite forming of the steel blank. The temperature of the blank in the mold is controlled to form it. After the forming is completed, the cooling process of the component is controlled to cause an alloy phase change, and after cooling, a steel shell component with a gradient distribution of microstructure and mechanical properties is obtained. The microstructure and mechanical properties such as strength and hardness of the steel shell component prepared by the present invention are gradiently distributed along the length direction, which meets the use requirements of the steel shell component, and the equipment used in the preparation process is conventional equipment and is easy to control.

The application discloses an ovarian tumor multi-parameter MRI benign and malignant identification method and system, relates to the field of image processing technology, and comprises the following steps: registering T2 weighting, diffusion weighting and its apparent diffusion coefficient, and dynamic contrast enhancement sequence to a unified coordinate system taking T2 weighting as a reference; generating a solid component significance map by multiplicative gating according to diffusion restriction of the apparent diffusion coefficient, early enhancement of the dynamic enhancement and T2 solid tissue signals, and suppressing cystic area contribution; performing local fine registration on the solid component by taking the map as a position-by-position weight, and performing sequence-specific gain weighting fusion on each sequence under spatial gating; outputting a malignant probability representing benign and malignant tendency as auxiliary identification information according to the fusion features, and decomposing the malignant probability into contribution amounts of papillary projection, low apparent diffusion coefficient of the solid area and a washout type of time-signal intensity curve to output explainable evidence.

This present invention concerns a single use aseptic kit comprising: a disaggregation module for receipt and processing of material comprising solid mammalian tissue; and a stabilization module for storing disaggregated product material, wherein each of said modules comprises one or more flexible containers connected by one or more conduits adapted to enable flow of the tissue material there between; and wherein each of said modules comprises one or more ports to permit aseptic input of media and / or reagents into the one or more flexible containers. The invention further relates to an automated device for semi-automated aseptic disaggregation and / or enrichment and / or stabilisation of cells or cell aggregates from mammalian solid tissue comprising a programmable processor and the single use aseptic kit. The invention further relates to a semi-automatic aseptic tissue processing method.

Disclosed herein are methods for manufacturing tissue-interfacing assemblies, such as solid needles, that include one or more therapeutic agents. In some embodiments, a method for manufacturing a tissue-interfacing assembly includes compressing a granular therapeutic agent within a mold cavity of a mold to form a solid tissue-interfacing assembly. The mold cavity can define an elongated shape extending along a longitudinal axis from an opening of the mold cavity to a distal end of the mold cavity, and the granular therapeutic agent can be compressed by moving a mold punch along the longitudinal axis toward the distal end. After compressing the granular therapeutic agent to form the solid tissue-interfacing assembly, the tissue-interfacing assembly can be removed from the mold and subsequently inserted into tissue to deliver the therapeutic agent to a subject.

The invention provides a tumor sampling device for oncology department, which comprises a sampling module, the sampling module is arranged on the inner side of a fixed cylinder, the sampling module comprises a solid sampling mechanism and a liquid sampling mechanism, and the solid sampling mechanism and the liquid sampling mechanism synchronously sample solid tissues and liquid at a tumor; the solid sampling mechanism comprises a grabbing assembly and a cutting assembly, the grabbing assembly is connected with the cutting assembly, the grabbing assembly is used for grabbing solid tissue at the tumor, and the cutting assembly is used for cutting the grabbed solid tissue. Solid tissue can be grabbed through the grabbing assembly, a living body grabbed by the grabbing assembly can be cut through the cutting assembly, discomfort brought to a patient during hard grabbing is reduced, more solid tissue samples can be obtained at a time, the situation that secondary sampling is needed due to the fact that few samples are obtained in a scraping mode is avoided, and the sampling efficiency is improved. And when the grabbing assembly is moved out, the cut samples can be conveniently taken out.

Disclosed are systems and methods for simultaneous detection of DNA and RNA genetic alterations comprising gene splicing variants, mutations, indel, copy number changes, fusion and combination thereof, in a biofluid sample from the patient without physically separating RNA from DNA. The systems and methods are similarly applicable to the simultaneous detection of DNA and RNA genetic alterations in solid tissues comprising gene splicing variants, mutations, indel, copy number changes, fusion and combination thereof. The present method utilized a barcoding method for analysis. The streamlined methods improve the simplicity, quantification accuracy and detection sensitivity and specificity of non-invasive detections of biomarkers.