15 results about "Solid tissue" patented technology

Apparatuses and methods for recovering solid tissue and dislodged cells (“D-cells”) from a biopsy are disclosed herein. In an embodiment, a biopsy container apparatus for recovering solid tissue and D-cells from a biopsy includes a receiving container and a sieve container. The receiving container has a receiving chamber with an upper opening and a watertight bottom surface. The sieve container is configured to be located at least partially within the receiving chamber and removed from the receiving chamber through the upper opening. The sieve container has a bottom sieve surface including a plurality of apertures configured to pass the D-cells from the biopsy but not the solid tissue from the biopsy into the receiving chamber after the solid tissue and the D-cells from the biopsy are placed into the sieve container.

Apparatuses and methods for recovering solid tissue and dislodged cells ("D-cells") from a biopsy are disclosed herein. In an embodiment, a biopsy container apparatus for recovering solid tissue and D-cells from a biopsy includes a receiving container and a sieve container. The receiving container has a receiving chamber with an upper opening and a watertight bottom surface. The sieve container is configured to be located at least partially within the receiving chamber and removed from the receiving chamber through the upper opening. The sieve container has a bottom sieve surface including a plurality of apertures configured to pass the D-cells from the biopsy but not the solid tissue from the biopsy into the receiving chamber after the solid tissue and the D-cells from the biopsy are placed into the sieve container.

The application provides a method for separating nucleic acid binding proteins in solid tissue cells, and the method comprises the following steps: step S1, preparing solid tissue into a cell suspension 1; step S2, cross-linking the cell suspension 1 by using a cross-linking agent to form a cell suspension 2 with nucleic acid-NABPs complexes; step S3, lysing red blood cells in the cell suspension 2 of step S2 to remove high-abundance protein interference; step S4, after the cells treated in step S3 are lysed by using an organic reagent, the nucleic acid-NABPs complexes in the solid tissue cells are selectively enriched by using affinity chromatography; and step S5, after the nucleic acid-NABPs complexes obtained in step S4 are de-cross-linked, nucleic acid binding proteins (NABPs) in the solid tissue cells are obtained.

The present invention provides a gastrointestinal fluid decompressor, comprising a collection box, a suction assembly, wherein the suction assembly is mounted on the upper portion of the collection box; a filter screen connected to the side wall of the inner cavity of the collection box, and used to filter and process the gastrointestinal fluid; and a crushing mechanism, wherein the crushing mechanism comprises a crushing mesh cylinder, wherein two symmetrically distributed crushing mesh cylinders are connected to the bottom of the filter screen, a crushing assembly is disposed inside the crushing mesh cylinder, and a lifting assembly is disposed outside the crushing assembly. The present invention utilizes the filter screen to filter the gastrointestinal fluid, utilizes the crushing assembly to crush solid tissue in the filtered gastrointestinal fluid, and utilizes the lifting assembly to lift the crushed solid tissue filtered by the crushing mesh cylinder and send it into the crushing assembly for further crushing, and repeats the process multiple times until all solid tissue is crushed, thereby preventing larger solid tissue from clogging the discharge port of the collection box or other processing equipment, and facilitating subsequent processing of the gastrointestinal fluid.

The application discloses a kind of negative pressure suction gun, manual negative pressure suction method, it is related to biomedical engineering technical field, the gun handle of negative pressure suction gun is reciprocated and opened and closed to drive suction pumping section to reciprocate in negative pressure sedimentation cavity Negative pressure suction and extrusion pumping, negative pressure sedimentation cavity can pass through liquid suction pipe one-way liquid suction when negative pressure suction, negative pressure sedimentation cavity can be one-way liquid delivery to the liquid storage part when extrusion pumping, so as to be used for suction liquid and soft solid tissue in liquid by setting negative pressure sedimentation cavity, then by adjusting increase negative pressure suction force, the viscosity of solid tissue that can be sucked is also greater, so that the negative pressure suction gun of the application can not only be used for endometrial gynecological field liquid suction and solid tissue sampling, but also can be used for closed narrow space tumor biopsy, brain tissue and other solid-liquid components suction, during suction process, by rotating liquid suction pipe, the inlet slope of liquid suction pipe is realized 360 degree rotation, to avoid suction dead angle.

The utility model discloses a crushing and separating device for plant extraction, which belongs to the technical field of plant extraction and comprises an outer barrel body and a feed port arranged in the middle of the upper end of the outer barrel body, a separating barrel is mounted in the middle of the inner side of the outer barrel body, and a first discharge pipe is arranged at the lower end of the separating barrel. A second discharging pipe is installed at the lower end of an area defined by the separation barrel and the outer barrel body, a servo motor is installed on the side edge of the upper end of the outer barrel body, a power gear is installed at the output end of the servo motor, a linkage gear ring is arranged on the side edge of the power gear, and the linkage gear ring is fixed to the separation barrel; and a movable frame is mounted on the edge of the inner side of the separation barrel. According to the crushing and separating device for plant extraction, plants can be effectively crushed, meanwhile, when crushed plant tissue fluid is separated, plant solid tissue attached to the net barrel can be cleaned, and holes in the net barrel are prevented from being blocked by the solid tissue.

Disclosed herein are devices and methods for recovering solid tissue and exfoliated cells ("D-cells") from biopsy. In an embodiment, a biopsy container device includes a sample collection container, a basket screen, and a buffer container. The sample collection container includes a reagent chamber. The basket screen is configured for at least partially removable attachment within the sample collection container, and the basket screen includes a screen surface configured to permit passage of exfoliated cells from the biopsy but not permit passage of solid tissue from the biopsy. The buffer container is configured for removable attachment to a sample collection container, and the buffer container includes a buffer chamber.

The invention provides a multiple myeloma detection system based on single cell in-situ sequencing. The CosMxSMI technology is applied to hematologic tumors for the first time, limitation of solid tissues is broken through, and synchronous analysis of cell types, RNA and protein in-situ expression in a bone marrow microenvironment is achieved. A single-cell suspension sample smaller than 1 mL is used, common protein targets are subjected to flow analysis based on RNA FISH and MM of an MM-related customized panel, and information such as bone marrow or peripheral blood single-cell-level cell types, proportions, RNA and protein expression profiles and the like can be obtained in situ on a slide microarray at the same time. Traditional FISH, flow cytometry and GEP step-by-step detection are replaced, manpower of the pathology department and a blood cell chamber is saved, the sample collection amount, loss and operation errors are reduced, and the clinical report time is shortened by 80%.

The invention relates to the technical field of tissue preservation solutions, and provides a solid tissue flow detection sample preservation solution and a preparation method thereof, and the preservation solution comprises N-acetylcysteine, melatonin, lactobionic acid and an HBSS buffer solution. NAC, melatonin and lactobionic acid jointly act on an antioxidant pathway, ROS is removed, accumulation of lipid peroxidation products (MDA) is reduced, the mitochondrial function is protected, and the cell survival rate is increased. The melatonin and lactobionic acid act together to inhibit ferroptosis paths, maintain the health state of cells and improve the cell survival rate and the stability of antigen epitopes. All the components and a stable environment provided by HBSS act together to maintain the high activity of cells and the stability of antigen epitopes.

The utility model discloses a drainage bag used in an ICU (Intensive Care Unit) operation, particularly relates to the technical field of medical equipment, and comprises a drainage bag body, a liquid outlet communicated and connected with the bottom of the drainage bag body and a liquid inlet catheter communicated and connected with the top of the drainage bag body, and the drainage end of the liquid inlet catheter is communicated and connected with a connector. The cleaning device has the advantages that the sealing ball is jacked open under the action of pressure by pressing the perfusion device, cleaning liquid enters the inner side of the flushing pipe through the perfusion head, and then tissue solids attached to the filter plate are cleaned through the flushing head; the cleaned solid tissues are discharged through the liquid outlet end and are received by a container, and after the perfusion apparatus is pulled out, the sealing ball is reset by utilizing the elastic acting force of the spring, so that the drainage bag body can be effectively prevented from being blocked in the human body tissue fluid drainage process, medical personnel do not need to replace the drainage bag body, and the operation is simple and convenient. And precious time is gained for rescuing the critically ill patients.

The present invention relates to a method for isolating and culturing tissue-resident uPAR+ / Nestin+ stem cells, and a use thereof, and provides a method for isolating, on the basis of uPAR-plasmin activity, tissue-resident stem cells present in solid tissues, wherein the uPAR-plasmin activity of stem cells has been suggested to have a close relationship with stem cell growth, migration ability, physiological activity and differentiation ability, and thus can be used in a method for isolating high-potency stem cells, the uPAR+ stem cells isolated from solid tissues are applicable to the production of cell therapeutic agents, tissue engineering therapeutic agents, and novel biopharmaceuticals using secretions including exosomes, and thus have high industrial applicability, and the present invention is applicable to fundamental cell biology and molecular biology research and novel drug development research that relate to the cell division, migration, growth, and differentiation of tissue-resident stem cells.

The invention relates to the technical field of cell targeted therapy, in particular to a construction method and application of a cell robot for enhancing cell therapy targeting and entity penetration. The invention relates to a construction method of a cell robot for enhancing cell therapy targeting and entity penetration. The construction method comprises the following steps: coupling magnetic microfilaments on the surfaces of cells; the magnetic microfilament comprises a microfilament and magnetic particles attached to the microfilament; and the microfilaments are made of polylactic acid-glycolic acid copolymer. According to the technical scheme, the technical problem that in the prior art, chimeric antigen receptor modified macrophages with high targeting and penetrating capacity are lacked can be solved, and the technical problems that in the prior art, chimeric antigen receptor cells generally face infiltration of solid tissues and infiltration is not ideal are further solved. The engineering cell design combining the bionic sperm model and magnetic driving breaks through the bottleneck of the traditional cell therapy in solid tissue delivery, provides a brand new technical thought and method for wider cell therapy optimization, and has ideal application and popularization values.

Disclosed herein are methods for manufacturing tissue-interfacing assemblies, such as solid needles, that include one or more therapeutic agents. In some embodiments, a method for manufacturing a tissue-interfacing assembly includes compressing a granular therapeutic agent within a mold cavity of a mold to form a solid tissue-interfacing assembly. The mold cavity can define an elongated shape extending along a longitudinal axis from an opening of the mold cavity to a distal end of the mold cavity, and the granular therapeutic agent can be compressed by moving a mold punch along the longitudinal axis toward the distal end. After compressing the granular therapeutic agent to form the solid tissue-interfacing assembly, the tissue-interfacing assembly can be removed from the mold and subsequently inserted into tissue to deliver the therapeutic agent to a subject.

The invention discloses a single-port laparoscope solid tissue sampler, and relates to the technical field of medical instruments, the single-port laparoscope solid tissue sampler comprises a connecting unit, the connecting unit comprises a connecting shell, a sampling tube rotatably arranged in an inner cavity of the connecting shell, a clamping assembly arranged in an inner cavity of the sampling tube, and a first driving assembly arranged between the connecting shell and the sampling tube; and the second driving assembly is arranged between the connecting shell and the clamping assembly. The device has the advantages that the kidney mesh bag is expanded through the expansion unit, the number of operation participants is reduced by adopting a mechanical fixing mode, the kidney mesh bag can be uniformly expanded through the expansion unit, the situation that a blade mistakenly touches the kidney mesh bag can be further reduced, a window area is formed between the expansion unit and the blade, and the operation efficiency is improved. The cutting condition can be observed in real time and adjusted in time, the blade is controlled in a mechanical mode, the operation difficulty of doctors can be lowered, and most doctors can complete sampling operation.

The application discloses an array electrode group electrode independent control device and a control method, relates to the technical field of electronic medical treatment, and comprises a radio frequency generating device, a radio frequency isolation voltage division module, an electrode independent control module, an array electrode group, a microcontroller, a control module, an input and display module; the radio frequency isolation voltage division module is used for isolating and dividing a radio frequency source into radio frequency ablation signals with the same frequency, the same phase and different voltage values through a high-frequency transformer; the array electrode group is used for transmitting radio frequency treatment signals and applying the radio frequency signals to solid tissues; the microcontroller is used for realizing a radio frequency treatment system and controlling the work of the array electrode group; and the control module is used for controlling the radio frequency voltage or power applied to the array electrode group in real time and the electrode work mode. The array electrode group electrode independent control device provided by the application is simple to operate, convenient to use, can accurately control irregularly shaped tissues to perform conformal ablation and accurate boundary temperature control, and has high practicability.