Experimental primary cell extraction device

Abstract

The utility model discloses a primary cell extraction device for experiments, and relates to the technical field of experimental apparatuses. The device comprises a grinding cylinder, a buckling cover body and a stirring mechanism, the buckling cover body is arranged on the surface of an opening of the grinding cylinder and is in buckling connection with the surface of the opening of the grinding cylinder to form a sealing structure, the stirring mechanism comprises a driving motor, a matched bevel gear and a linkage rod, and the driving motor is installed on one side of the surface of the buckling cover body. Through the arrangement of the grinding cylinder, the buckling cover body and the stirring mechanism, the driving motor is started, the driving bevel gear drives the linkage bevel gear and the matching bevel gear to move in opposite rotating directions, efficient cutting is carried out in the grinding cylinder through the stirring blade, and rapid smashing of solid tissue is achieved; meanwhile, a plurality of groups of grinding rods are driven by the grinding frame to sufficiently grind the tissue blocks, and the ground tissue is diluted and discharged by utilizing flushing fluid after grinding, so that a cell solution is collected, cells are more sufficiently digested, and the collection rate of the cells is improved.

Description

Technical Field

[0001] The utility model belongs to the technical field of experimental equipment, in particular to a primary cell extraction device for experiments. Background Art

[0002] Primary culture refers to the first culture of tissues or cells removed from the body, also called primary culture. Primary culture has a short in vitro time, and its genetic characteristics are similar to those of cells in the body, making it suitable for studies on cell morphology, function, and differentiation. Primary separation and extraction refers to the process of isolating a single cell suspension from tissues in the body; generally, animal tissues are removed surgically, the tissues are manually cut into pieces, and then digested and filtered to obtain primary cells, which are then cultured in vitro for cell experimental research.

[0003] In order to obtain enough primary cells, it is often necessary to process larger solid tissues, but manual cutting and shredding greatly increases the workload and easily increases the risk of primary cell contamination, and it is necessary to consider low-cost consumable devices suitable for novices. Utility Model Content

[0004] The purpose of the utility model is to provide a primary cell extraction device for experiments, which solves the problem that in order to obtain sufficient primary cells, it is often necessary to process larger solid tissues, while manual operation and shredding greatly increase the workload and easily increase the risk of primary cell contamination, and it is necessary to consider a low-cost consumable device suitable for novices.

[0005] In order to solve the above technical problems, the utility model is realized by the following technical solutions:

[0006] The utility model discloses a primary cell extraction device for experiments, comprising a grinding cylinder, a snap-fit ​​cover body and a stirring mechanism, wherein the snap-fit ​​cover body is arranged on the surface of the opening of the grinding cylinder, snap-fitted with the grinding cylinder, and forms a sealing structure, wherein the stirring mechanism comprises a driving motor, a matching bevel gear and a linkage rod, wherein the driving motor is installed on one side of the surface of the snap-fit ​​cover body, one end of the driving motor shaft is fixedly connected with a connecting rod, the connecting rod is arranged inside the snap-fit ​​cover body, rotationally matched with the connecting rod, and forms a sealing structure, one end of the connecting rod is fixedly connected with a driving bevel gear, the linkage rod is arranged inside the snap-fit ​​cover body, rotationally matched with the connecting rod, and forms a sealing structure, and the outer side surface of the other end of the linkage rod is fixedly connected with a linkage bevel gear, the linkage bevel gear is arranged in the snap-fit ​​cover body, and the linkage bevel gear meshes with the driving bevel gear.

[0007] The outer side surface of one end of the linkage rod is fixedly connected with a plurality of stirring blades, and the stirring blades are arranged in the grinding cylinder.

[0008] The matching bevel gear is arranged inside the buckling cover body, and is installed on the outside of the linkage rod and rotationally matched therewith, and the matching bevel gear is meshed with the driving bevel gear.

[0009] The interior of the mating bevel gear is fixedly connected with a connecting sleeve rod, the connecting sleeve rod is arranged on the outside of the linkage rod and rotatably cooperates with it, one end of the connecting sleeve rod is fixedly connected with a grinding frame, and the grinding frame is arranged in the grinding cylinder.

[0010] A plurality of groups of grinding rods are fixedly connected to the peripheral side surface of the grinding frame.

[0011] The connecting sleeve rod and the linkage rod are both of a clamping rod structure; through such an arrangement, the connecting sleeve rod and the linkage rod parts inside the grinding cylinder are detachable, so that after the extraction work is completed, the connecting sleeve rod and the linkage rod parts inside the grinding cylinder and the stirring blade and the grinding rod can be removed and replaced, thereby improving the safety of the device during cell extraction and avoiding contamination caused by repeated use.

[0012] The utility model has the following beneficial effects:

[0013] The utility model provides a grinding cylinder, a buckled cover body and a stirring mechanism, starts a driving motor, drives the linked bevel gear and the matching bevel gear to make opposite-direction movements, and uses a stirring blade to efficiently cut in the grinding cylinder to achieve rapid crushing of solid tissues; at the same time, the grinding frame drives multiple groups of grinding rods to fully grind tissue blocks, and after grinding, the ground tissue is diluted and discharged with a flushing liquid, and finally the cell solution is collected, so that the cells are more fully digested and the cell collection rate is improved.

[0014] Of course, any product implementing the present invention does not necessarily need to achieve all of the advantages described above at the same time. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] In order to more clearly illustrate the technical solutions of the embodiments of the utility model, the drawings required for describing the embodiments are briefly introduced below. Obviously, the drawings described below are only some embodiments of the utility model. For ordinary technicians in this field, other drawings can be obtained based on these drawings without creative work.

[0016] Figure 1 It is a three-dimensional structural schematic diagram of the utility model;

[0017] Figure 2 It is a left view of the structure of the utility model;

[0018] Figure 3 for Figure 2 Schematic diagram of the AA section structure.

[0019] In the accompanying drawings, the components represented by the reference numerals are listed as follows:

[0020] 1. Grinding cylinder; 2. Snap-fit ​​cover; 3. Stirring mechanism; 301. Driving motor; 302. Connecting rod; 303. Driving bevel gear; 304. Linking bevel gear; 305. Connecting sleeve rod; 306. Matching bevel gear; 307. Linking rod; 308. Grinding frame; 309. Stirring blade; 310. Grinding rod. DETAILED DESCRIPTION

[0021] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments of the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0022] In the description of the present invention, it should be understood that the terms "upper", "middle", "outer", "inner" and the like indicating directions or positional relationships are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the components or elements referred to must have a specific direction, be constructed and operated in a specific direction, and therefore should not be understood as limitations on the present invention.

[0023] In the description of the present invention, it should be noted that, unless otherwise clearly specified and limited, the terms "installed", "provided with", "connected", etc. should be understood in a broad sense. For example, "connected" can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection, or it can be indirectly connected through an intermediate medium, or it can be the internal communication of two components. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0024] See also Figure 1-Figure 3As shown, the utility model is a primary cell extraction device for experiment, including a grinding cylinder 1, a snap-fit ​​cover body 2 and a stirring mechanism 3. The snap-fit ​​cover body 2 is arranged on the surface of the opening of the grinding cylinder 1, and is snap-fitted with it to form a sealing structure. The stirring mechanism 3 includes a driving motor 301, a matching bevel gear 306 and a linkage rod 307. The driving motor 301 is installed on one side of the surface of the snap-fit ​​cover body 2. One end of the rotating shaft of the driving motor 301 is fixedly connected with a connecting rod 302. The connecting rod 302 is arranged inside the snap-fit ​​cover body 2, and is rotatably matched with it to form a sealing structure. One end of the connecting rod 302 is fixedly connected with a driving bevel gear 303. The linkage rod 307 is arranged inside the snap-fit ​​cover body 2, and is rotatably matched with it to form a sealing structure. The outer side surface of the other end of the linkage rod 307 is fixedly connected with a linkage bevel gear 304. The linkage bevel gear 304 is arranged in the snap-fit ​​cover body 2, and the linkage bevel gear 304 is meshed with the driving bevel gear 303.

[0025] A plurality of stirring blades 309 are fixedly connected to the outer side surface of one end of the linkage rod 307 , and the stirring blades 309 are disposed in the grinding cylinder 1 .

[0026] The matching bevel gear 306 is disposed inside the buckled cover body 2 and is installed on the outside of the linkage rod 307 and rotatably matched therewith. The matching bevel gear 306 is meshed with the driving bevel gear 303 .

[0027] A connecting sleeve rod 305 is fixedly connected to the inside of the matching bevel gear 306. The connecting sleeve rod 305 is arranged on the outside of the linkage rod 307 and rotates with it. One end of the connecting sleeve rod 305 is fixedly connected to a grinding frame 308, and the grinding frame 308 is arranged in the grinding cylinder 1.

[0028] A plurality of groups of grinding rods 310 are fixedly connected to the peripheral side surface of the grinding frame 308 .

[0029] The connecting sleeve rod 305 and the linkage rod 307 are both of a snap-on rod structure; through such an arrangement, the connecting sleeve rod 305 and the linkage rod 307 inside the grinding cylinder 1 are detachable, so that after the extraction work is completed, the connecting sleeve rod 305 and the linkage rod 307 inside the grinding cylinder 1 and the stirring blade 309 and the grinding rod 310 can be removed and replaced, thereby improving the safety of the device during cell extraction and avoiding contamination caused by repeated use.

[0030] It should be noted that when the technical solution is actually used, the solid tissue and the flushing liquid are placed in the grinding cylinder 1, the buckled cover 2 is buckled, and then the drive motor 301 is started, and the driving bevel gear 303 drives the linkage bevel gear 304 and the matching bevel gear 306 to make opposite direction movements, and the stirring blade 309 is used to perform efficient cutting in the grinding cylinder 1 to achieve rapid crushing of the solid tissue; at the same time, the grinding frame 308 drives multiple groups of grinding rods 310 to fully grind the tissue blocks, and the flushing liquid is used to dilute and discharge the ground tissue, and finally the cell solution is collected to make the cells more fully digested and improve the cell collection rate. After the extraction work is completed, the connecting sleeve 305 and the linkage rod 307 in the grinding cylinder 1 and the stirring blade 309 and the grinding rod 310 are disassembled and replaced to improve the safety of the device during cell extraction and avoid contamination caused by repeated use.

[0031] In the description of this specification, the description with reference to the terms "one embodiment", "example", "specific example", etc. means that the specific features, structures, materials or characteristics described in conjunction with the embodiment or example are included in at least one embodiment or example of the utility model. In this specification, the schematic representation of the above terms does not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described can be combined in any one or more embodiments or examples in a suitable manner.

[0032] The preferred embodiments of the utility model disclosed above are only used to help explain the utility model. The preferred embodiments do not describe all the details in detail, nor do they limit the utility model to the specific implementation methods described. Obviously, many modifications and changes can be made according to the content of this specification. This specification selects and specifically describes these embodiments in order to better explain the principles and practical applications of the utility model, so that technicians in the relevant technical field can well understand and use the utility model. The utility model is limited only by the claims and their full scope and equivalents.

Claims

1. A primary cell extraction device for experiments, comprising a grinding cylinder (1), a snap-fitting cover (2) and a stirring mechanism (3), characterized in that: The snap-fit ​​cover (2) is arranged on the surface of the opening of the grinding cylinder (1) and snap-fitted therewith to form a sealing structure. The stirring mechanism (3) comprises a driving motor (301), a matching bevel gear (306) and a linkage rod (307). The driving motor (301) is installed on one side of the surface of the snap-fit ​​cover (2). One end of the rotating shaft of the driving motor (301) is fixedly connected to a connecting rod (302). The connecting rod (302) is arranged inside the snap-fit ​​cover (2) and snap-fitted therewith. They are rotatably matched and form a sealing structure. One end of the connecting rod (302) is fixedly connected to a driving bevel gear (303). The linkage rod (307) is arranged inside the buckled cover body (2) and is rotatably matched therewith to form a sealing structure. The outer side surface of the other end of the linkage rod (307) is fixedly connected to a linkage bevel gear (304). The linkage bevel gear (304) is arranged inside the buckled cover body (2), and the linkage bevel gear (304) is meshed with the driving bevel gear (303).

2. The primary cell extraction device for experiments according to claim 1, characterized in that: The outer side surface of one end of the linkage rod (307) is fixedly connected to a plurality of groups of stirring blades (309), and the stirring blades (309) are arranged in the grinding cylinder (1).

3. The primary cell extraction device for experiments according to claim 2, characterized in that: The matching bevel gear (306) is arranged inside the buckled cover body (2) and is installed on the outside of the linkage rod (307) and is rotationally matched therewith. The matching bevel gear (306) is meshed with the driving bevel gear (303).

4. The primary cell extraction device for experiments according to claim 3, characterized in that: A connecting sleeve rod (305) is fixedly connected to the interior of the matching bevel gear (306); the connecting sleeve rod (305) is arranged on the outside of the linkage rod (307) and rotatably cooperates with it; one end of the connecting sleeve rod (305) is fixedly connected to a grinding frame (308); the grinding frame (308) is arranged in the grinding cylinder (1).

5. The primary cell extraction device for experiments according to claim 4, characterized in that: A plurality of groups of grinding rods (310) are fixedly connected to the peripheral side surface of the grinding frame (308).

6. The primary cell extraction device for experiments according to claim 5, characterized in that: The connecting sleeve rod (305) and the linkage rod (307) are both clamping rod structures.

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