Asexual Rapid Propagation Method of Maca Hybrid

A technology for hybrids and maca, applied in the biological field, can solve the problems of character separation, loss of heterosis and excellent characters of maca hybrids, etc., and achieve the effects of improving the quality of seedlings, being easy to standardize, and improving economic benefits.

Inactive Publication Date: 2016-10-26
会泽枢康中草药种植有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The technical problem to be solved by the present invention is to provide a kind of asexual rapid propagation method of Maca hybrids for fixed, Preserve its heterosis and develop maca breeding to lay a technical foundation, and at the same time provide high-quality hybrid seedlings with consistent genotype background to meet the needs of artificial planting

Method used

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  • Asexual Rapid Propagation Method of Maca Hybrid
  • Asexual Rapid Propagation Method of Maca Hybrid
  • Asexual Rapid Propagation Method of Maca Hybrid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] 1. Acquisition of maca hybrid explants

[0038] Select healthy maca hybrid plants with good growth potential, no pests and diseases, and no deformities. Take the young inflorescences in the afternoon on a sunny day. At this time, the surface of the plants is clean and dry, and the content of bacteria and fungi is low; wash the young inflorescences with tap water first The unclean matter and dust on the surface were rinsed under running water for 2 h, placed on the ultra-clean workbench, and the young lazy inflorescences were taken out of the beaker one by one with scorched tweezers, and put into the beaker with a volume ratio of 75%. Disinfect in alcohol for 15 s, then use 0.1% HgCl 2 (mercuric chloride) (mass ratio) sterilized for 5 min, and finally rinsed with sterile water for 8 times to remove residual mercury chloride on the inflorescences of young larks, and then placed on sterile filter paper to absorb the surface moisture; shake the vessel;

[0039] 2. Callus ...

Embodiment 2

[0076] 1. Acquisition of maca hybrid explants The acquisition and treatment of maca hybrid explants described in this example are the same as in Example 1.

[0077] 2. Callus induction The composition of the callus induction medium A is: B 5 Basic culture medium, zeatin ZT 1.0 mg / L, naphthalene acetic acid NAA 0.1 mg / L, sucrose 20000 mg / L, agar powder 4800 mg / L, pH 5.4; culture method and other requirements are the same as in Example 1.

[0078] 3. The composition of the clump seedling generation and proliferation medium B is: B 5 Basic culture medium, 6-benzylaminopurine 6-BA 1.0mg / L, naphthaleneacetic acid NAA 1.5 mg / L, zeatin ZT 0.1mg / L, sucrose 20000 mg / L, agar 4800 mg / L, pH 5.4; Method and other requirements are the same as embodiment one.

[0079]4. The composition of the proliferation medium C is: MS basic culture medium, 6-benzylaminopurine 6-BA 1.0 mg / L, naphthalene acetic acid NAA 1.5 mg / L, zeatin ZT 1.0 mg / L, sucrose 30000 mg / L, agar powder 4800 mg / L, pH 5.8; Al...

Embodiment 3

[0083] Embodiment 3: The acquisition, culture method, steps and culture conditions of the maca hybrid explants in this embodiment are all the same as in Embodiment 2, and the components of each medium are different, as follows:

[0084] (1) Callus induction medium A: B 5 Basic culture medium, rice protein ZT 2.0mg / L, naphthaleneacetic acid NAA 0.01mg / L, sucrose 20000 mg / L agar 4800 mg / L, pH 5.8;

[0085] (2) Germination and Proliferation Medium B: B 5 Basic culture medium, 6-benzylaminopurine 6-BA 1.5mg / L, naphthaleneacetic acid NAA 1.8 mg / L, zeatin ZT 1.0mg / L, sucrose 20000 mg / L, agar powder 4800 mg / L, pH 5.8;

[0086] (3) Medium C: MS basic culture medium, 6-benzylaminopurine 6-BA 1.5 mg / L, naphthaleneacetic acid NAA 1.8 mg / L, zeatin ZT 0.1 mg / L, sucrose 30000 mg / L, agar powder 4800 mg / L, pH 5.8;

[0087] (4) Rooting medium D: 1 / 2 MS basic culture medium, naphthalene acetic acid NAA 0.3 mg / L, indole butyric acid IBA 0.1 mg / L, activated carbon AC 1.5 mg / L, sucrose 15000 m...

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Abstract

The invention discloses a fast asexual propagation method for maca hybrid. The method comprises the following steps: inoculating a sterilized maca hybrid explant into a callus inducing medium, generating callus, and the generated callus into a clump seedling generating and breeding medium to cultivate; when clump seedlings are generated and bred to a certain quantity, carrying out subculture multiplication in the breeding medium; when the quantity of the bred seedlings reaches the rooting requirements, transferring healthy main seedlings in the clump seedlings into a rooting medium to cultivate; and when the seedlings are healthy and the roots are thick, carrying out hardening-seedling for 3 days according to a conventional method, and transplanting into a floating plate with disinfection humus and cultivating for 60 days so as to obtain the healthy maca hybrid clonal seedlings. The production technology of the maca hybrid clonal seedlings is optimized and adjusted; after the callus and the clump seedlings are obtained, MS with high NH<4+> and B5 with low NH<4+> are adopted as basic culture mediums for alternative use in enrichment culture; the phenomena that a maca test-tube plantlet is easy to age and yellow are thoroughly solved; the asexual propagation cycle is shortened; the breeding efficiency is improved; and an effective way is provided for breeding of F1 generation of high-quality seedlings of the maca hybrid.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a technology for asexual rapid propagation of a medicinal and edible plant maca hybrid. Background technique [0002] Maca Lepidium meyenii The genus Brassicaceae (Brassicaceae) Lepidium ) is an annual herbaceous plant, native to the Andes in Peru at an altitude of more than 3500 m. It can grow normally in the unique environment of no fertilizer, lack of oxygen, large temperature difference between day and night, and long-term ice. Maca is often referred to as "Peruvian Ginseng" because it rivals ginseng in terms of nutritional content and energy and stamina-boosting properties. Because maca absorbs a large amount of soil nutrients during its growth, under the original farming conditions, the land after harvesting maca roots needs to be left fallow for several years before it can be planted again. Therefore, maca was once on the verge of extinction and was listed as ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 王枢
Owner 会泽枢康中草药种植有限公司
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