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Artificial efficient propagation method for primula denticulata

A ball flower, artificial technology, applied in gardening methods, botanical equipment and methods, gardening tools/equipment, etc., can solve the problems of difficult large-scale promotion, slow growth of seedlings, low seed germination rate, etc. The effect of culture cycle, fast proliferation and high production efficiency

Pending Publication Date: 2021-02-02
YUNNAN UNIV OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the seed germination rate is low, the seedlings grow slowly and have small biomass, and the variation of offspring is large, and limited by the germination season, it is difficult to carry out large-scale, standardized (consistent genotype background) planting and large-scale promotion of high-efficiency varieties

Method used

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  • Artificial efficient propagation method for primula denticulata
  • Artificial efficient propagation method for primula denticulata
  • Artificial efficient propagation method for primula denticulata

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Such as figure 1 , figure 2 , image 3 and Figure 4 Shown, a kind of method for the artificial high-efficiency propagation of Primula primula in northern Yunnan comprises the following steps:

[0047] 1. Acquisition of explants: Select robust plants with good growth potential, no pests and diseases, and no deformities, and take the leaves of the year and remove the petioles.

[0048] 2. Wash the leaves in step 1 with running water, soak them in 10% washing powder solution by mass ratio for 5 minutes, rinse them with running water for 30 minutes, and place them in an ultra-clean workbench; soak them in 75% ethanol solution by volume for 3 seconds, and then wash them with 0.1% ethanol solution by mass ratio. % mercuric chloride aqueous solution for 5 minutes, and finally rinsed with sterile water for 5 times, each time not less than 3 minutes, and placed on filter paper to absorb the residual sterile water. Shake the utensils well throughout the sanitization process...

Embodiment 2

[0063] Such as figure 1 , figure 2 , image 3 and Figure 4 Shown, a kind of method for the artificial high-efficiency propagation of Primula primula in northern Yunnan comprises the following steps:

[0064] 1. Acquisition of explants: Select robust plants with good growth potential, no pests and diseases, and no deformities, and take the leaves of the year and remove the petioles.

[0065] 2. Wash the leaves in step 1 with running water, soak them in 10% washing powder solution by mass ratio for 5 minutes, rinse them with running water for 30 minutes, and place them in an ultra-clean workbench; soak them in 75% ethanol solution by volume for 3 seconds, and then wash them with 0.1% ethanol solution by mass ratio. % mercuric chloride aqueous solution for 5 minutes, and finally rinsed with sterile water for 5 times, each time not less than 3 minutes, and placed on filter paper to absorb the residual sterile water. Shake the utensils well throughout the sanitization process...

Embodiment 3

[0079] Such as figure 1 , figure 2 , image 3 and Figure 4 Shown, a kind of method for the artificial high-efficiency propagation of Primula primula in northern Yunnan comprises the following steps:

[0080] 1. Acquisition of explants: Select robust plants with good growth potential, no pests and diseases, and no deformities, and take the leaves of the year and remove the petioles.

[0081] 2. Wash the leaves in step 1 with running water, soak them in 10% washing powder solution by mass ratio for 5 minutes, rinse them with running water for 30 minutes, and place them in an ultra-clean workbench; soak them in 75% ethanol solution by volume for 3 seconds, and then wash them with 0.1% ethanol solution by mass ratio. % mercuric chloride aqueous solution for 5 minutes, and finally rinsed with sterile water for 5 times, each time not less than 3 minutes, and placed on filter paper to absorb the residual sterile water. Shake the utensils well throughout the sanitization process...

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Abstract

The invention discloses an artificial efficient propagation method for primula denticulata. The method comprises the following steps of inoculating disinfected and sterilized primula denticulata leafexplants into a culture medium A for culture, carrying out callus induction and clump bud occurrence culture under the conditions that the illuminance is 1800-2300lx, the illumination time is 8h / d andthe temperature is controlled to be 20+ / -1 DEG C, transferring induced clump buds into a fresh culture medium A for culturing after 30 days, and enabling the adventitious bud propagation coefficientto reach about 20.00 after 30 days; and when the height of the proliferated clump buds is 5-8 cm, inoculating single seedlings into a culture medium B, culturing for 25-30 days to obtain rooting seedlings with strong seedlings and thick roots, and then transplanting the rooting seedlings. The method is low in cost, short in period and high in test-tube plantlet quality and survival rate, providestechnical support for protecting wild resources and breeding high-quality plantlets, and can be used for producing excellent plantlets with consistent genotype backgrounds in a short time.

Description

technical field [0001] The invention relates to the technical field of propagation of Chinese herbal medicines, in particular to a method for artificial and efficient propagation of Primula chinensis in northern Yunnan. Background technique [0002] Primula denticulata (Primula denticulata) is a perennial herb of the genus Primula (Primulaceae) in the family Primulaceae. The scapes of Primula denticulata in North Yunnan are usually thicker, more than 6 times as long as the leaves, and the corolla is also slightly larger , the distribution area is not connected with the original subspecies. The flowering period is March-April, and the fruiting period is April. Produced in Yunnan, western Sichuan and other places. Born in hillside grassland and shrubs, 1500-3000 meters above sea level. [0003] Primula primula in northern Yunnan is the basic plant of wild ginseng, also known as rice umbrella flower (Guizhou folk medicine) and primula (Yunnan Chinese herbal medicine). It is...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/008
Inventor 黄衡宇曾彪陈建颖
Owner YUNNAN UNIV OF TRADITIONAL CHINESE MEDICINE
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