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Artificial efficient propagation method for primula denticulata

A ball flower and artificial technology, applied in horticultural methods, botany equipment and methods, gardening tools/equipment, etc., can solve problems such as difficult large-scale promotion, slow growth of seedlings, low seed germination rate, etc., to achieve shortening The effect of culture cycle, fast proliferation speed and high production efficiency

Inactive Publication Date: 2020-11-20
云南华农农业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the seed germination rate is low, the seedlings grow slowly and have small biomass, and the variation of offspring is large, and limited by the germination season, it is difficult to carry out large-scale, standardized (consistent genotype background) planting and large-scale promotion of high-efficiency varieties

Method used

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  • Artificial efficient propagation method for primula denticulata
  • Artificial efficient propagation method for primula denticulata
  • Artificial efficient propagation method for primula denticulata

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] A method for artificial high-efficiency propagation of primula in northern Yunnan, comprising the following steps:

[0054] 1. Acquisition of explants: Select robust plants with good growth potential, no pests and diseases, and no deformities, and take the leaves of the year and remove the petioles.

[0055]2. Wash the leaves in step 1 with running water, soak them in 10% washing powder solution by mass ratio for 5 minutes, rinse them with running water for 10 hours, and place them in an ultra-clean workbench; soak them in 75% ethanol solution by volume for 3 seconds, and then wash them with 0.1% ethanol solution by mass ratio. % mercuric chloride aqueous solution for 5 minutes, and finally rinsed with sterile water for 5 times, each time not less than 3 minutes, and placed on filter paper to absorb the residual sterile water. Shake the utensils well throughout the sanitization process.

[0056] 3. Callus induction and cluster bud formation culture: insert the leaves t...

Embodiment 2

[0070] A method for artificial high-efficiency propagation of primula in northern Yunnan, comprising the following steps:

[0071] 1. Acquisition of explants: Select robust plants with good growth potential, no pests and diseases, and no deformities, and take the leaves of the year and remove the petioles.

[0072] 2. Wash the leaves in step 1 with running water, soak them in 10% washing powder solution by mass ratio for 5 minutes, rinse them with running water for 10 hours, and place them in an ultra-clean workbench; soak them in 75% ethanol solution by volume for 3 seconds, and then wash them with 0.1% ethanol solution by mass ratio. % mercuric chloride aqueous solution for 5 minutes, and finally rinsed with sterile water for 5 times, each time not less than 3 minutes, and placed on filter paper to absorb the residual sterile water. Shake the utensils well throughout the sanitization process.

[0073] 3. Callus induction and cluster bud formation culture: insert the leaves ...

Embodiment 3

[0087] A method for artificial high-efficiency propagation of primula in northern Yunnan, comprising the following steps:

[0088] 1. Acquisition of explants: Select robust plants with good growth potential, no pests and diseases, and no deformities, and take the leaves of the year and remove the petioles.

[0089] 2. Wash the leaves in step 1 with running water, soak them in 10% washing powder solution by mass ratio for 5 minutes, rinse them with running water for 10 hours, and place them in an ultra-clean workbench; soak them in 75% ethanol solution by volume for 3 seconds, and then wash them with 0.1% ethanol solution by mass ratio. % mercuric chloride aqueous solution for 5 minutes, and finally rinsed with sterile water for 5 times, each time not less than 3 minutes, and placed on filter paper to absorb the residual sterile water. Shake the utensils well throughout the sanitization process.

[0090] 3. Callus induction and cluster bud formation culture: insert the leaves ...

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Abstract

The invention provides an artificial efficient propagation method for primula denticulate. The method comprises the following steps: a culture medium A is inoculated with leaf explants of primula denticulate after sterilization treatment for culture, and callus induction and cluster bud generation culture are carried out under the conditions that the illuminance is 1800-2300 lx, illumination lastsfor 8 h / d and temperature is controlled at 20 + / - 1 DEG C; after 30 d, induced cluster buds are transferred in a fresh culture medium A, and after 30 d, the propagation coefficient of adventitious buds is up to about 20.00. When the proliferated cluster buds are 5-8 cm high, a culture medium B is inoculated with single seedlings for culture, strong rooting seedlings with thick roots are obtainedaft er 25-30 d, and then, the rooting seedlings are transplanted. The method is low in cost, short in cycle and high in quality and survival rate of test-tube plantlets, provides technical support forprotecting wild resources and high-quality seedling propagation, and can produce excellent seedlings with consistent genotype background in a short time.

Description

technical field [0001] The invention relates to the technical field of propagation of Chinese herbal medicines, in particular to a method for artificially high-efficiency propagation of primula primula in northern Yunnan. Background technique [0002] Primula primula in northern Yunnan is a perennial herb of the genus Primula in the family Primulaceae. This subspecies is a type that extends to Yunnan, Sichuan, Guizhou and northern Myanmar in my country. Although it is indistinguishable from the original subspecies Separate characters, but the scape is usually thicker, more than 6 times as long as the leaf cluster, the corolla is also slightly larger, and the distribution area is not connected with the original subspecies. The flowering period is March-April, and the fruiting period is April. Produced in Yunnan (Tengchong, Longling, Yongping, Maguan, Dali, Lijiang, Shuangbai, Yimen, Kunming), western Sichuan (Shimian, Puge, Luding) and Guizhou (Guiyang, Shuicheng, Leishan) an...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 陈建颖曾彪黄衡宇
Owner 云南华农农业有限公司
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