Tissue culture method for strawberry transplant seedlings

Abstract

Strawberry transplanting seedling tissue culture method, the steps include: A, selection of strawberry explants, B, disinfection and sterilization, C, anti-browning culture, D, callus induction, cluster bud generation and proliferation culture, E, expansion Propagation and cultivation, F, rooting and cultivation, G, seedling hardening and transplanting. The beneficial effect is that the tissue culture procedure of strawberry seedlings is simple, and callus induction, cluster bud formation and proliferation culture can be carried out simultaneously in the same medium; the tissue culture period is short, the reproduction coefficient is high, and the whole rapid propagation process only needs three kinds of culture The problem of browning, callus, cluster bud generation and proliferation, and rooting can be solved based on the basis; adventitious root induction of test-tube seedlings does not add phytohormones, and the survival rate of hardened seedlings is high and grows rapidly; seedlings can maintain the same genotype, Progeny are not prone to browning, and there is little virus accumulation; the tissue culture method of inhibiting browning is low in cost, short in time, and the seedlings produced are of good quality, high survival rate, and stable properties, which can meet the industrial production of high-quality strawberry seedlings need.

Description

technical field [0001] The invention belongs to the technical field of tissue culture of strawberry seedlings, and relates to a tissue culture method for transplanting strawberry seedlings. Background technique [0002] Strawberry is traditionally propagated mainly by stolons and ramets, both of which have problems such as long cycle and low coefficient. Long-term asexual reproduction can easily cause the virus to accumulate in the strawberry plant, resulting in the degradation of the strawberry species and the decrease in yield year by year. The use of tissue culture technology for detoxification and rapid propagation is an effective way to solve these problems. However, in the existing tissue culture technology, the overall application of tissue culture seedlings is at a low level, and the explant browning phenomenon of strawberry tissue culture seedlings is serious, the reproduction coefficient is low, the tissue culture period is long, and the transplanting survival rat...

AI Technical Summary

Problems solved by technology

[0003] The present invention aims at the problems of serious browning phenomenon, low reproduction coefficient, long tissue culture cycle and low transplanting survival rate in the prior art in tissue culture technology of strawberry seedlings, and provides a method for tissue culture of strawberry transplanted seedlings, and its technical scheme Yes: