Tissue culture method for strawberry transplant seedlings

A strawberry and tissue culture technology, applied in horticultural methods, botanical equipment and methods, horticulture and other directions, can solve the problems of browning, low transplant survival rate, long tissue culture period, etc. Breeding cycle, improve economic efficiency, good effect

Inactive Publication Date: 2016-09-28
玉溪市祥馨农业技术开发有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The present invention aims at the problems of serious browning phenomenon, low reproduction coefficient, long tissue culture cycle and low transplanting survival rate in the prior art in tissue culture technology of strawberry seedlings, and provides a method for tissue culture of strawberry transplanted seedlings, and its technical scheme Yes:

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Embodiment one: a kind of strawberry transplanting seedling tissue culture method comprises the following steps:

[0024] A, the selection of strawberry explants: select the robust strawberry plants with good growth, no damage by diseases and insect pests, and no deformities, get its stolons with jointed stem segments, cut into about 3 cm long with a scalpel, and obtain strawberry explants;

[0025] B. Disinfection and sterilization: wash the explants with tap water to clean the soil and dust on the surface, then soak the explants in a washing powder solution with a mass ratio of 10% for 10 minutes, shake and stir slightly, rinse with running water for 30 minutes, and place Put it in the container on the ultra-clean workbench; then soak it in a vitamin C solution with a mass ratio of 50% for 3 minutes, treat it with an ethanol solution with a volume ratio of 75% for 13 seconds, and then sterilize it with a mercury aqueous solution with a mass ratio of 0.1% for 9 minutes ...

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PUM

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Abstract

Strawberry transplanting seedling tissue culture method, the steps include: A, selection of strawberry explants, B, disinfection and sterilization, C, anti-browning culture, D, callus induction, cluster bud generation and proliferation culture, E, expansion Propagation and cultivation, F, rooting and cultivation, G, seedling hardening and transplanting. The beneficial effect is that the tissue culture procedure of strawberry seedlings is simple, and callus induction, cluster bud formation and proliferation culture can be carried out simultaneously in the same medium; the tissue culture period is short, the reproduction coefficient is high, and the whole rapid propagation process only needs three kinds of culture The problem of browning, callus, cluster bud generation and proliferation, and rooting can be solved based on the basis; adventitious root induction of test-tube seedlings does not add phytohormones, and the survival rate of hardened seedlings is high and grows rapidly; seedlings can maintain the same genotype, Progeny are not prone to browning, and there is little virus accumulation; the tissue culture method of inhibiting browning is low in cost, short in time, and the seedlings produced are of good quality, high survival rate, and stable properties, which can meet the industrial production of high-quality strawberry seedlings need.

Description

technical field [0001] The invention belongs to the technical field of tissue culture of strawberry seedlings, and relates to a tissue culture method for transplanting strawberry seedlings. Background technique [0002] Strawberry is traditionally propagated mainly by stolons and ramets, both of which have problems such as long cycle and low coefficient. Long-term asexual reproduction can easily cause the virus to accumulate in the strawberry plant, resulting in the degradation of the strawberry species and the decrease in yield year by year. The use of tissue culture technology for detoxification and rapid propagation is an effective way to solve these problems. However, in the existing tissue culture technology, the overall application of tissue culture seedlings is at a low level, and the explant browning phenomenon of strawberry tissue culture seedlings is serious, the reproduction coefficient is low, the tissue culture period is long, and the transplanting survival rat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/00A01H4/008
Inventor 黄衡宇李继祥王元忠张霁李培源
Owner 玉溪市祥馨农业技术开发有限公司
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