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New method for artificial rapid propagation of cremastra appendiculata (D.Don) Makino

The technology of a kind of Shan Arzuki mushroom and new method is applied in the field of artificial rapid propagation of Shan Arzu mushroom, which can solve the problems such as long division propagation cycle, limited planting scale, slow breeding process, etc., and achieves improvement of seedling quality, low solution coefficient, The effect of widening and stretching leaves

Active Publication Date: 2019-11-19
YUNNAN UNIV OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The technical problem to be solved by the present invention is to provide a new method for rapid artificial propagation of Arthrobus chinensis to solve the problems of long reproductive cycle, low efficiency, slow breeding process and extremely limited planting scale in the artificial cultivation of Arthrowia chinensis

Method used

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  • New method for artificial rapid propagation of cremastra appendiculata (D.Don) Makino
  • New method for artificial rapid propagation of cremastra appendiculata (D.Don) Makino
  • New method for artificial rapid propagation of cremastra appendiculata (D.Don) Makino

Examples

Experimental program
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Effect test

Embodiment 1

[0071] A new method of artificial and rapid propagation of Shanzi mushroom includes the following steps:

[0072] 1. Obtaining explants: Choose a mushroom plant with excellent phenotypic traits, and take the pseudobulb as the explant.

[0073] 2. Clean the surface soil of the fake bulb in step 1 with tap water first, then soak it in 10% detergent solution (mass ratio) for 10 minutes and slightly shake and stir, rinse with running water for 30 minutes, and place it on an ultra-clean workbench; 5% vitamin C (VC) ) Solution (mass ratio) soaked for 3 minutes, treated with 75% ethanol solution (volume ratio) for 13 seconds, then sterilized with 0.1% mercury aqueous solution (mass ratio) for 9 minutes, and finally rinsed with sterile water 6 times, each time for 3 minutes. Shake the dish thoroughly during the entire sterilization process.

[0074] 3. Obtaining aseptic seedlings: The pseudobulbs that have been sterilized in step 2 are cut into 2cm size with a scalpel according to the bud p...

Embodiment 2

[0090] A new method of artificial and rapid propagation of Shanzi mushroom includes the following steps:

[0091] 1. Obtaining explants: Choose a mushroom plant with excellent phenotypic traits, and take the pseudobulb as the explant.

[0092] 2. Clean the surface soil of the fake bulb in step 1 with tap water first, then soak it in 10% detergent solution (mass ratio) for 10 minutes and slightly shake and stir, rinse with running water for 30 minutes, and place it on an ultra-clean workbench; 5% vitamin C (VC) ) Solution (mass ratio) soaked for 3min, treated with 75% ethanol solution (volume ratio) for 15s, then sterilized with 0.1% mercury aqueous solution (mass ratio) for 8min, and finally rinsed with sterile water 5 times, 3min each time. Shake the dish thoroughly during the entire sterilization process.

[0093] 3. Obtaining aseptic seedlings: The pseudobulbs that have been disinfected and sterilized in step 2 are cut into 1.7cm size with a scalpel according to the bud points, a...

Embodiment 3

[0109] A new method of artificial and rapid propagation of Shanzi mushroom includes the following steps:

[0110] 1. Obtaining explants: Choose a mushroom plant with excellent phenotypic traits, and take the pseudobulb as the explant.

[0111] 2. Clean the surface soil of the fake bulb in step 1 with tap water first, then soak it in 10% detergent solution (mass ratio) for 10 minutes and slightly shake and stir, rinse with running water for 30 minutes, and place it on an ultra-clean workbench; 5% vitamin C (VC) ) Solution (mass ratio) soaked for 3 minutes, treated with 75% ethanol solution (volume ratio) for 14 seconds, then sterilized with 0.1% mercury aqueous solution (mass ratio) for 10 minutes, and finally rinsed with sterile water 5 times, each time for 3 minutes. Shake the dish thoroughly during the entire sterilization process.

[0112] 3. Obtaining aseptic seedlings: The pseudobulbs that have been disinfected and sterilized in step 2 are cut into a size of 1.6 cm with a scalp...

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Abstract

The invention discloses a new method for artificial rapid propagation of cremastra appendiculata (D.Don) Makino.The method comprises the following steps: (1) obtaining an explant; (2) firstly washingsoil on the surface of pseudobulb in step (1) with tap water, then soaking with a washing powder solution, then rinsing with running water, then treating with an ethanol solution, and rinsing after disinfection; (3) obtaining sterile seedlings; (4) transferring young shoots grown from buds cultured in step (3) to a medium A in step (3) for enrichment culture, cutting off seedlings with callus, andcontinuing to transfer to a medium B for culture; (5) inoculating a medium C with single seedlings with rice grain-sized pseudobulb after subculture in step (4) for culture; (6) exercising the seedlings and transplanting. The method disclosed by the invention has the advantages that the in-vitro rapid propagation of the cremastra appendiculata (D.Don) Makino is optimized and adjusted; the processof artificial rapid propagation is simplified; the propagation efficiency is greatly improved; the cost is low and the time is short; not only is the survival rate of tube seedlings through seedlingexercising improved, but the nursery cycle is also greatly shortened.

Description

Technical field [0001] The present invention belongs to the field of biotechnology, and specifically relates to a new method for artificial and rapid propagation of Shanzi mushroom. Background technique [0002] Cremastra appendiculata (D.Don) Makino, also known as Rhododendron, is a perennial herbaceous plant in the Orchidaceae genus Cremastra. It grows in ditch wetlands or under forest wetlands on hillsides, at an altitude of 500- 2900m, usually produced in Yunnan Gongshan, Fengqing, Yuxi and other places; the distribution range includes Tibet, Guizhou, southern Shaanxi and southwestern Shanxi in China, and also in Nepal, Bhutan, India, Vietnam, Thailand and Japan abroad. distributed. [0003] Shanzi mushroom is used as medicine with fake bulbs. It is one of the original plants of Shanzi mushroom. It is sweet, slightly pungent, and has a small poison. Efficacy, internal use can resist liver cancer, breast cancer, uterine cancer, etc., external use to treat sore poison, snake an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 黄衡宇徐福荣
Owner YUNNAN UNIV OF TRADITIONAL CHINESE MEDICINE
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