A kind of high-efficiency artificial seedling raising method of Passiflora high-quality hybrid

A technology for hybrids and passionflowers, which is applied in horticultural methods, botanical equipment and methods, horticultural tools/equipment, etc., and can solve the problems of low-level application of tissue culture seedlings, inability to fix, and large separation of traits.

Active Publication Date: 2021-05-07
YUNNAN UNIV OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Traditionally, Passiflora is mainly propagated by seeds and cuttings. Seed seedlings have problems such as low germination rate, long seedling period, low survival rate, large separation of characters, and low robustness; while cuttings also have long cycle and Low survival rate and other problems, and long-term asexual reproduction is easy to cause virus accumulation, resulting in the degradation of passionflower species, the decline in variety quality, and the production decline year by year
These unfavorable factors have restricted the development of passionflower planting and processing industry, making my country's passionflower have no market competitiveness in the world
In Xishuangbanna Manfeilong Farm, Yunnan, a hybrid F 1 During the generation, a hybrid (new variety has not yet been applied for) has been selected with strong disease resistance, self-compatibility, purple-red fruit, fruit setting rate of more than 70%, and strong fruit juice fragrance and high sweetness; The variety is very unstable, and the above-mentioned excellent traits cannot be fixed by sexual reproduction.
There are many reports on tissue culture of various varieties of Passiflora, but there are still some problems in terms of reproduction coefficient and cycle, yellowing and defoliation of bottle seedlings, transplanting survival rate and high cost, so that the overall application of tissue culture seedlings is still at a low level. Level

Method used

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  • A kind of high-efficiency artificial seedling raising method of Passiflora high-quality hybrid
  • A kind of high-efficiency artificial seedling raising method of Passiflora high-quality hybrid
  • A kind of high-efficiency artificial seedling raising method of Passiflora high-quality hybrid

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Embodiment 1

[0081] A high-efficiency artificial seedling raising method for Passiflora high-quality hybrids, comprising the following steps:

[0082] 1. Acquisition of explants: Select a robust hybrid F with good growth potential, no pests and diseases, and no deformed fruit. 1 Substitute plants, whichever leaves the current year.

[0083] 2. Clean the dust and sundries on the surface of the leaves in step 1 with tap water, then soak them in 10% washing powder solution (mass ratio) for 10 minutes, shake and stir slightly, then rinse them with running water for 30 minutes, and place them on an ultra-clean workbench with a volume ratio of Disinfect with 75% alcohol for 15s, then use 0.1% HgCl 2 Sterilize for 4 minutes, and finally rinse with sterile water for 5 times, 3 minutes each time. Shake the utensils well throughout the sanitization process.

[0084] 3. Callus induction, cluster bud formation and proliferation: take the leaves that have been sterilized in two steps as materials, c...

Embodiment 2

[0115] A high-efficiency artificial seedling raising method for Passiflora high-quality hybrids, comprising the following steps:

[0116] 1. Acquisition of explants: Select a robust hybrid F with good growth potential, no pests and diseases, and no deformed fruit. 1 Substitute plants, whichever leaves the current year.

[0117] 2. Clean the dust and sundries on the surface of the leaves in step 1 with tap water, then soak them in 10% washing powder solution (mass ratio) for 10 minutes, shake and stir slightly, then rinse them with running water for 30 minutes, and place them on an ultra-clean workbench with a volume ratio of Disinfect with 75% alcohol for 15s, then use 0.1% HgCl 2 Sterilize for 4 minutes, and finally rinse with sterile water for 6 times, 3 minutes each time. Shake the utensils well throughout the sanitization process.

[0118] 3. Callus induction, cluster bud formation and proliferation: take the leaves that have been sterilized in two steps as materials, c...

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Abstract

The invention provides a high-efficiency artificial seedling-raising method of high-quality passionflower hybrids, which comprises the steps of: inoculating the sterilized leaf explants in a culture medium, inducing callus, forming clustered buds and proliferating , rooting induction, seedling hardening and transplanting. The present invention optimizes and adjusts the production process of hybrid seedlings of passionflower, synchronously carries out callus growth, cluster bud generation and cluster bud proliferation, simplifies the tissue culture process, and carries out three culture processes in one culture medium at the same time , greatly improving the reproductive efficiency. In addition, the invention solves the phenomena such as plant yellowing and leaf defoliation that are easy to occur in the tissue culture process of Passiflora plants, and has low cost, short cycle, high quality and high survival rate.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for high-efficiency artificial seedling cultivation of high-quality passionflower hybrids. Background technique [0002] Passiflora coerulea L., also known as passion fruit, egg fruit, Brazil fruit, is a perennial herbaceous to semi-woody vine climbing tropical and subtropical plant of Passifloraceae (Passifloraceae) Passiflora (Passiflora); Originated in South America from Brazil to Argentina, my country is mainly distributed in Taiwan, Guangdong, Fujian, Guangxi, Zhejiang, Sichuan, Yunnan and other provinces and regions. Passiflora is evergreen in four seasons, with gorgeous flower color, 5 petals, light green, outer corona lobes are filamentous, and inner corona fringe, its fruit is berry ovoid to nearly spherical, orange or yellow when ripe, good for storage Good luck, good taste, with the reputation of "king of juice". [0003] Passiflora is a cross-pollina...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 黄衡宇徐福荣
Owner YUNNAN UNIV OF TRADITIONAL CHINESE MEDICINE
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