Davallia mariesii breeding method

A technology of alpine fern teeth and formula, which is applied in the fields of fern propagation and flower cultivation, can solve the problems of high material requirements, difficulty in large-scale production, and large dosage, so as to improve economic benefits, strong adaptability, and stable seedling properties Effect

Inactive Publication Date: 2014-03-26
FLOWER RES INST OF YUNNAN ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to overcome the defects and deficiencies such as high material requirements, large dosage, and difficulty in large-scale production in the existing alpine fern seedling propagation technology, and provide a method and special Culture medium to meet the needs of large-scale production of high-quality alpine fern seedlings

Method used

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  • Davallia mariesii breeding method
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  • Davallia mariesii breeding method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] (1) Take the mature alpinia spores, wrap them with gauze, soak them in 75% alcohol for 30 seconds in an ultra-clean environment, and put them in a mercuric chloride solution with a mass fraction of 0.1% for disinfection. 6min, disinfected in sodium hypochlorite mixture for 10min, then rinsed with sterile water 4 to 5 times, each time for 2min, to obtain the aseptic spores of alpine fern. The sodium hypochlorite mixture is mixed with sodium hypochlorite solution and Tween 20 , The ratio of sodium hypochlorite solution and Tween 20 is: add 2 drops of Tween 20 to 100ml of sodium hypochlorite solution with a mass fraction of 2%.

[0049] (2) Induction and proliferation culture: the sterile spores obtained in step (1) were inserted into a culture bottle filled with the following induction proliferation medium:

[0050] MS Basic Medium

[0051]

[0052] Cultivate in dark for 15 days at a temperature of 23°C, then switch to a light intensity of 2000lx, a temperature of 23°...

Embodiment 2

[0061] Embodiment 2 is the same as Embodiment 1 except that the following measures are different, and will not be repeated. (1) Take the mature alpinia spores and wrap them with gauze, and soak them in 75% alcohol for 35 seconds in an ultra-clean environment.

[0062] (2) The induced proliferation medium for induction and proliferation culture is:

[0063] MS Basic Medium

[0064]

[0065] Cultivate in dark for 16 days at a temperature of 25°C, then switch to a light intensity of 1800lx, a temperature of 25°C, and a light time of 9h / d, and continue to cultivate until the spores of Alpinia alpiniae germinate and differentiate into green spheroids, and the green spheroids cut open.

[0066] (3) Subculture: 28 days per subculture, 3 times for subculture, the conditions of subculture are: light intensity is 1800lx, temperature is 25°C, light time is 9h / d, subculture is 27d , the medium for subculture is identical to the formulation of the induced proliferation medium describ...

Embodiment 3

[0073] Embodiment 3 is the same as Embodiment 1 except that the following measures are different, and will not be repeated.

[0074] (1) Take the mature alpinia spores and wrap them with gauze, then soak them in 75% alcohol for 40 seconds in an ultra-clean environment.

[0075] (2) The culture medium for inducing proliferation is:

[0076] MS Basic Medium

[0077]

[0078] Cultivate in the dark for 15 days at a temperature of 27°C, then switch to a light intensity of 1500lx, a temperature of 27°C, and a light time of 10h / d, and continue to cultivate until the spores of Alpinia alpiniae germinate and differentiate into green spheroids, and the green spheroids cut open.

[0079] (3) Subculture: 30 days per subculture, 5 subcultures in total, the subculture conditions are: light intensity 1500lx, temperature 27°C, light time 10h / d, subculture 30d, The medium formula of the subculture is the same as that of the induced proliferation medium described in step (2).

[0080] (4...

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Abstract

The invention discloses a Davallia mariesii breeding method, which comprises the following steps: an induction proliferation medium and a rooting medium are provided, the inducting and proliferation cultivating are concurrently carried out, after exercising seedling, and the seedling can be directly transplanted to a seedlings bag for culturing. The invention provides a technical scheme of a key link in a seedling breeding technology process of the Davallia mariesii, the proliferation rate is fast, the reproduction rates can reach 5-7 times during one period with 25-30 days; the rooting culture lasts about 20 days, each plant can breed 5-8 roots, the seedling is robust, the root develops, a tissue culture process is simplified, the cost and production time are greatly reduced, and the Davallia mariesii breeding method is suitable for standardization and industrial-scale production.

Description

technical field [0001] The invention belongs to the technical field of flower cultivation, in particular to the technical field of fern propagation. Background technique [0002] Alpine fern (Davallia mariesii) is a fern of the genus Drynariae in the Drynariaceae family. Originally produced in Guatemala, it entered China as cut leaves in the 1990s. It is a kind of fern suitable for cutting leaves. It is currently widely used in flower arrangement. Its various leaf shapes, leaf postures and green colors are pleasing to the eye. With the development of indoor ornamental plants, the supply of their products has been in short supply. Alpine fern propagation methods are mainly branch propagation and spore propagation, but the number of branch propagation is small, the plant type is easily damaged, the recovery is slow and limited by the season, and a large number of seedlings cannot be obtained in a short period of time; spore propagation is due to the spores in the natural env...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/04A01H4/00
Inventor 许凤李绅崇杨春梅曹桦汪国鲜李金泽单芹丽阮继伟
Owner FLOWER RES INST OF YUNNAN ACAD OF AGRI SCI
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