Tissue culture and rapid propagation method of million stars

A tissue culture and rapid technology, which is applied in the tissue culture and propagation of plants, in the field of tissue culture and rapid propagation of MillionStars, can solve the problems of large-scale promotion of fine varieties that are difficult to scale production, low propagation coefficient of twig cuttings, hundreds of Wanxing seeds are difficult to harvest and other problems, to achieve the effect of solving the complex source of parents, improving the quality of seedlings, and shortening the production cycle

Inactive Publication Date: 2011-01-19
FLOWER RES INST OF YUNNAN ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The conventional propagation methods of Million Stars include seed propagation and twig cuttings. The former has the problem that the seeds of Million Stars are difficult to harvest and their properties are unstable. The latter has the problem of low propagation coefficient of twig cuttings, so both methods are difficult. Carry out large-scale production and large-scale promotion of fine varieties
At present, there is no report on the tissue culture research of million stars, especially the large-scale asexual reproduction of million stars

Method used

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  • Tissue culture and rapid propagation method of million stars
  • Tissue culture and rapid propagation method of million stars

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] 1. Selection of explants: Select plants with many flowers, double petals in clusters, white color, hard branches, no pests and diseases, and no deformities from the blooming million-star plants, and then select plants with strong bases and no flower buds The shoot tip is an explant, and the outer leaves are peeled off;

[0038] 2. Sterilization of explants: cut the explants selected in step 1 into 1.5cm stem segments, soak them in conventional washing powder water for 3 minutes, rinse them with tap water, and place them in an ultra-clean workbench with a mass concentration of 0.1% Hgcl 2 Sterilize the solution for 25 minutes, then switch to 2% sodium hypochlorite solution (with an appropriate amount of Tween added) to sterilize for 15 minutes, and finally take it out and rinse it in sterile water for 3 times;

[0039] 3. Induction culture: inoculate the shoot tips after the above-mentioned disinfection and sterilization treatment in the following induction medium under...

Embodiment 2

[0068] 1. Selection of explants: Select plants with many flowers, double petals in clusters, white color, hard branches, no pests and diseases, and no deformities from the blooming million-star plants, and then select plants with strong bases and no flower buds Stem tips and axillary buds are explants, and the outer leaves are stripped off;

[0069] 2. Sterilization of explants: cut the explants selected in step 1 into 2 cm stem segments, soak them in conventional washing powder water for 3 minutes, rinse them with tap water, and place them in an ultra-clean workbench with a mass concentration of 0.1 % Hgcl 2 The solution was sterilized for 15 minutes, and then sterilized with a 2% sodium hypochlorite solution (with an appropriate amount of Tween added) for 20 minutes, and finally taken out and rinsed 4 times in sterile water;

[0070] 3. Induction culture: inoculate the shoot tips and axillary buds after the above-mentioned disinfection and sterilization treatment in the fol...

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Abstract

The invention provides a tissue culture and rapid propagation method of million stars, which comprises the process steps of: selecting an explant, sterilizing the explant, inducing and culturing an adventitious bud, carrying out enrichment culture on the adventitious bud, subculturing, rooting, carrying out transition transplanting and the like. The million stars is cultured on various corresponding culture media and is directly transplanted until growing into a rooting seedling, wherein the seed evolution rate can reach 90 percent, and the transplanting survival rate is above 95 percent. The method solves the problem on the key technology for establishing in a million stars sterile system, achieves the purposes of high seed entering efficiency, high propagation speed and stable production technology through comprehensive adjustment of hormone, nutrition and culture environment, and realizes massive, standardized and industrialized production of the million stars.

Description

technical field [0001] The invention relates to a plant tissue culture and propagation method, in particular to a tissue culture and rapid propagation method of MillionStars, belonging to the technical field of plant cultivation. Background technique [0002] Gypsophila is one of the most popular cut flowers in the world and one of the top ten cut flowers in Europe. In my country, it is mainly planted in Yunnan Province, with a planting area of ​​nearly 4,000 mu, and more than 3.5 million seedlings are needed every year. The new variety of Gypsophila "Million Stars" has thin branches, few leaves, and many and small flowers. In addition to being used as floral accessories, it is also a good material for producing dried flowers. Since this variety was introduced from the Netherlands in 2002, the Flower Research Institute of Yunnan Academy of Agricultural Sciences has been accepted by the market after years of trial planting and trial marketing. At present, "Million Star" cut...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00A01G1/00
Inventor 杨春梅吴丽芳屈云慧赵培飞汪国鲜单芹丽龙江
Owner FLOWER RES INST OF YUNNAN ACAD OF AGRI SCI
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