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Tide type bioreactor and method for culturing lily bulblets thereof

A bioreactor, lily seed ball technology, applied in the fields of botanical equipment and methods, horticultural methods, applications, etc., can solve the problems of high material and labor costs, difficult to scale production, complex production procedures, etc., and save manpower and material resources. , the seed ball is stable, and the effect of improving economic efficiency

Active Publication Date: 2014-10-01
FLOWER RES INST OF YUNNAN ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to overcome the problems of low efficiency, high material and labor costs, relatively complicated production procedures, and difficulty in large-scale production in the existing lily seed ball propagation technology

Method used

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  • Tide type bioreactor and method for culturing lily bulblets thereof
  • Tide type bioreactor and method for culturing lily bulblets thereof
  • Tide type bioreactor and method for culturing lily bulblets thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0058] Such as Figure 1~3 As shown, a tidal bioreactor includes a nutrient solution storage container 2, a culture container 1, an air pump 3, a timer 4, an air filter membrane 5, a water saving valve 6 and a liquid addition filter 7, and the nutrient solution storage container 2 is connected to the culture container 1 through a conduit equipped with a water-saving valve 6, and the nutrient solution storage container 2 and the culture container 1 are respectively connected to the corresponding air pump 3 through a conduit equipped with an air filter membrane 5, and the The aspirating pump 3 is connected with the timer 4, and the timer 4 controls whether the aspirating pump 3 operates; the culture container 1 is provided with a metal gauze 8 parallel to the bottom surface, and the nutrient solution storage container and the aspirating pump The conduit between is provided with liquid-adding filter 7.

[0059] When need soaking culture, add culture medium from liquid-adding fil...

Embodiment 2

[0090] The tidal bioreactor of this embodiment is the same as that of Embodiment 1.

[0091] The tidal bioreactor of the present embodiment cultivates the method for lily bulb, comprises the steps:

[0092] A. Select healthy growing lily balls with a diameter of 16-18cm, peel off the outer layer and the scales with spots and wormholes, separate the scales of the lily balls, wash them with washing powder water first, and then rinse them with clean water. After wiping the material twice with 75% alcohol in an ultra-clean environment, put it into a mercuric chloride solution with a mass concentration of 0.1% for disinfection for 15 minutes, then put it in the mixed solution for disinfection for 15 minutes; then rinse it with sterile water 3 times, 1 min each time, the lily explants were obtained, and the mixed solution was that every 100ml of mass concentration was 2% sodium hypochlorite solution, and 5 drops of Tween-20 were added dropwise to obtain the lily explants. Insert th...

Embodiment 3

[0123] The tidal bioreactor of this embodiment is the same as that of Embodiment 1.

[0124] The tidal bioreactor of the present embodiment cultivates the method for lily bulb, comprises the steps:

[0125] A. Select healthy growing lily balls with a diameter of 16-18cm, peel off the outer layer and the scales with spots and wormholes, separate the scales of the lily balls, wash them with washing powder water first, and then rinse them with clean water. After wiping the material twice with 75% alcohol in an ultra-clean environment, put it into a mercuric chloride solution with a mass concentration of 0.1% for disinfection for 15 minutes, then put it in the mixed solution for disinfection for 15 minutes; then rinse it with sterile water 3 times, 1 min each time, the lily explants were obtained, and the mixed solution was that every 100ml of mass concentration was 2% sodium hypochlorite solution, and 5 drops of Tween-20 were added dropwise to obtain the lily explants. Insert th...

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Abstract

The invention innovatively utilizes a tide type bioreactor to rapidly propagate lily transplanting small bulblets. The bulblets are cultured in a conventional solid propagation culture medium to grow to obtain the lily small bulblets with the diameter of 0.2-0.4cm to be used as explants; the explants are inoculated into the tide type bioreactor; a liquid culture medium formula is replaced in a culturing process of 50 days; medicines are added to control the pollution and bulblet expansion and rooting are gradually carried out; after the bulblets are discharged out of a bottle, the lily bulblets are managed by conventional vernalization, transplanting and planting methods. According to the invention, bulblet expansion and rooting culture are carried out gradually and a tissue culture procedure can be simplified; the manual cost in conventional solid tissue culture is greatly reduced; the generation of pollution and variant strains can be effectively controlled; the lily breeding period is shortened and the production cost is saved; the good properties of new species of the lilies can be rapidly fixed and kept; the difficulties that the propagating speed of the bulblets produced by a conventional lily propagation system is slow, the provenance is complicated and the quality of the bulblets is not stable are overcome.

Description

[0001] technical field [0002] The invention belongs to the technical field of plant tissue culture, and in particular relates to a tidal bioreactor and a method for cultivating lily balls. Background technique [0003] Lily is a perennial herbaceous bulb plant of the Liliaceae Lilium genus. It is a world-renowned ornamental flower. In recent years, with the development of my country's economy and the improvement of people's living standards, lily has become an important high-grade cut flower in my country's flower market. Widely used in garden greening, home decoration, festival flowers, medicinal food and other fields. With the continuous expansion of the lily demand market, fresh cut flower lilies, edible lilies and garden greening lily seed balls are widely planted in southwest, northwest, northeast and other regions of my country. The safety and rapid production and reproduction of lily seed bulbs as a provenance have also attracted more and more attention from the in...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 贾文杰蒋亚莲崔光芬赵培飞王祥宁段青马璐琳杜文文王继华
Owner FLOWER RES INST OF YUNNAN ACAD OF AGRI SCI
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