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Out-of-bottle bag rooting method for tissue culture seedlings of Europe and America hybrid aspens

A technology for tissue culture seedlings and hybrids, which is applied in horticultural methods, botanical equipment and methods, plant regeneration, etc., can solve the problems of high cultivation cost, long production cycle, and low survival rate of tissue culture seedling transplanting, and achieves simplified cultivation. Procedures, low production costs, the effect of reducing energy and man-hour consumption

Inactive Publication Date: 2013-02-13
NORTHEAST FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The present invention aims to solve the problems of high culture cost, long production cycle and low survival rate of transplanted tissue cultured seedlings in the existing European and American hybrid poplar tissue culture process, and provides a method for rooting European and American hybrid poplar tissue culture seedlings in the outer bag of the bottle

Method used

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  • Out-of-bottle bag rooting method for tissue culture seedlings of Europe and America hybrid aspens
  • Out-of-bottle bag rooting method for tissue culture seedlings of Europe and America hybrid aspens
  • Out-of-bottle bag rooting method for tissue culture seedlings of Europe and America hybrid aspens

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specific Embodiment approach 1

[0021] Specific embodiment one: the method for rooting in the outer bag of the European and American hybrid poplar tissue culture seedling bottle of the present embodiment comprises the following steps:

[0022] 1. Seal the bottom of a polypropylene plastic bag with a width of 17-20 cm and a height of 10-12 cm with a sealing machine to make a rooting culture bag;

[0023] 2. Mix peat soil and sand at a volume ratio of 1 to 2:1, and then sterilize at 121°C for 1.5 to 2 hours to obtain culture material, and then divide the culture material into rooting culture bags, each rooting culture bag The amount of medium culture medium is 1 / 3 of the volume of the culture bag;

[0024] 3. Dig a hole with the culture material in the culture bag. The size of the hole is 1.5-2cm wide and 1.5cm deep. The distance between the holes is 2cm. The dosage is 3~5mL;

[0025] 4. Take out the 1.5-3cm European and American hybrid poplar tissue culture seedlings from the culture medium, remove the call...

specific Embodiment approach 2

[0028] Specific embodiment two: the difference between this embodiment and specific embodiment one is that in step one, the bottom of the polypropylene plastic bag with a width of 18 cm and a height of 11 cm is plastic-sealed with a sealing machine. Others are the same as in the first embodiment.

specific Embodiment approach 3

[0029] Embodiment 3: The difference between this embodiment and Embodiment 1 or 2 is that in Step 2, peat soil and sand are mixed in a volume ratio of 1:1. Others are the same as in the first or second embodiment.

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Abstract

The invention relates to an out-of-bottle bag rooting method for tissue culture seedlings of Europe and America hybrid aspens. The method is to the problem that the cultivation cost is high, the production phase is long and the survival rate of transplanting of tissue culture seedlings is low in a tissue culture process of the Europe and America hybrid aspens. The method comprises the following steps: 1, plastically packaging the bottom of polypropylene plastic bags with a sealing machine to manufacture rooting culture bags; 2, mixing peat soils with sands, sterilizing at high speed to obtain composts and packing the composts into the rooting culture bags; 3, burrowing in the composts in the culture bags and wetting each hole with rooting culture solution; and 4, taking out the tissue culture seedlings of the Europe and America hybrid aspens from culture mediums, planting the tissue culture seedlings into the holes of the culture bags, sealing the top of the culture bags with the sealing machine, placing in greenhouses to culture for 5-7 days, gashing the top of the culture bags and then culturing for 8-10 days to obtain the rooting seedlings of the Europe and America hybrid aspens. The method is used in the field of asexual propagation of the Europe and America hybrid aspens.

Description

technical field [0001] The invention relates to a rooting method for European and American hybrid poplar tissue culture seedlings. Background technique [0002] European and American poplar (Populus tremula ×P.tremuloides) is a hybrid of European poplar (P.tremula) and North American poplar (P.tremuloides), which has fast growth, cold resistance, barren tolerance, tall tree shape, and strong adaptability It is an excellent tree species for afforestation in temperate and cold mountainous areas. In addition, the hybrid material is soft, dense, white, and the trunk is straight. It is a high-quality raw material for papermaking, plywood, particleboard and other industries. [0003] Studies have shown that: in Finland, European and American aspen hybrids are suitable for mountain afforestation, and plain afforestation grows better, and its rotation period and growth speed are faster than general mountain coniferous and broad-leaved afforestation species. However, the climate an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 刁桂萍王秋玉詹亚光杨传平闫绍鹏尚洁张荣沭
Owner NORTHEAST FORESTRY UNIVERSITY
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