One-step seedling rapid propagation method for tissue culture of Chinese cabbage

A technology for tissue culture and cabbage cultivation, applied in the field of plant tissue culture, can solve the problems of cumbersome pathways, low regeneration frequency, easy mutation of germplasm, etc., achieves short regeneration period, maintains the characteristics of the original species, and does not affect the proliferation rate. Effect

Active Publication Date: 2016-09-07
RIZHAO POLYTECHNIC
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Problems solved by technology

However, this approach is cumbersome, the germplasm is prone to mutation, and the regeneration frequency is not high

Method used

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  • One-step seedling rapid propagation method for tissue culture of Chinese cabbage
  • One-step seedling rapid propagation method for tissue culture of Chinese cabbage
  • One-step seedling rapid propagation method for tissue culture of Chinese cabbage

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specific Embodiment approach 1

[0017] Specific implementation mode 1: This implementation mode will further describe and illustrate the present invention in conjunction with specific examples below. Described method is carried out according to the following steps:

[0018] Step 1, take the leaves of Chinese cabbage that are free from diseases and insect pests and grow robustly as explants, and then sterilized;

[0019] Step 2, induction of leaf callus: cut the sterilized leaves into 0.4-0.6cm area 2 Inoculate it on the culture medium after being blocky, place the culture medium at a temperature of 25±2°C after inoculation, and cultivate it in complete darkness, and cultivate it under light until it takes root after 7 to 10 days;

[0020] Step 3. Domestication and transplanting of test-tube seedlings: Take out the rooted seedlings and put them in a hardening room with sufficient light. After 3 to 5 days, open the bottle and continue to harden the seedlings for 2 to 3 days; then take out the test-tube seedli...

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Abstract

The invention discloses a one-step seedling rapid propagation method for tissue culture of Chinese cabbage, which belongs to the technical field of plant tissue culture. The invention solves the problems of low coefficient, slow growth and difficulty in large-scale production in conventional propagation methods of the cabbage. The method is carried out according to the following steps: Step 1, disinfection and sterilization; Step 2, one-step seedling cultivation; Step 3, domestication and transplanting of test-tube seedlings: take out the rooted seedlings and put them in a seedling hardening room with sufficient light After 3 to 5 days, open the bottle mouth and continue to harden the seedlings for 2 to 3 days; then take out the test tube seedlings, wash the root culture medium, and transplant them into the substrate. The substrate is composed of peat soil and vermiculite, and the weight of peat soil and vermiculite The ratio of parts is 1:1, and the seedlings are sprayed 2-3 times a day for the first 7 days. The invention is used for tissue culture of Chinese cabbage.

Description

technical field [0001] The invention relates to a one-step seedling rapid propagation method for tissue culture of Chinese cabbage, belonging to the technical field of plant tissue culture. Background technique [0002] At present, the production and propagation of Chinese cabbage is mainly based on division propagation and cutting propagation. The details are as follows: 1. Cuttage propagation selects strong branches that grow in the same year, cuts into 10-15cm long stem segments, removes the base leaves, leaves 3.4 leaves in each stem segment, tops, inserts in the furrow, fills the soil with 3-5cm, and pours it once Water permeable, generally takes 7-10 days to take root and survive, the branches are best cut and planted, and can be transplanted and planted after 20 days. It can also be cut directly in the field, the planting density is 25cm row spacing, 15cm hole spacing, 2-3 plants per hole. 2. Propagation by ramets Dig up the roots of the Chinese cabbage to be ramete...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 李媛侯可雷
Owner RIZHAO POLYTECHNIC
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