One-step seedling and efficient in-vitro propagation method with gynura bicolor leaves

A technology for in vitro propagation of Gelonia purple-backed, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of long seedling breeding cycle, easy variation of germplasm, labor-intensive and time-consuming problems, and achieve the growth of seedlings in clusters Long and strong, good growth effect

Inactive Publication Date: 2013-07-17
ZHAOQING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It can be seen that the rapid propagation technology of G. purple-backed so far still uses the traditional tissue culture method. It has to go through multiple stages such as primary culture, subculture, rooting culture and seedling hardening and transplanting. The cycle is long, the germplasm is prone to variation, and the cost is high.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] (1) Medium preparation: MS was used as the basic medium, 0.25 mg of 6-BA, 0.4 mg of NAA, 0.2 mg of IBA, 7 g of carrageenan, and 30 g of sucrose were added per liter, and the pH of the medium was adjusted to 6.0 after mixing ; Dispense each bottle with about 30 mL, put it in an autoclave with sterilization conditions set at 121°C and 105 Kpa for 15 minutes, take out the culture bottle, and cool it for later use;

[0032] (2) Explant treatment and inoculation: According to the conventional inoculation method, the leaves of aseptic seedlings of G. purple-backed seedlings with a leaf diameter of about 0.8-1.5 cm were cut as explants, and the leaves were directly inoculated on the above-mentioned medium (MS+ 0.25 mg / L 6-BA+0.4 mg / L NAA+0.2 mg / L IBA+30 g / L sucrose+7 g / L carrageenan), inoculate 2 explants in each bottle, and aseptically seal the mouth of the bottle;

[0033] (3) Cultivation: Move the inoculated culture bottle into the culture room for culture, the culture cond...

Embodiment 2

[0036] Other operations are the same as in Example 1, except that in step (1), the formula of the medium is (MS+0.25 mg / L 6-BA+0.6 mg / L NAA+0.2 mg / L IBA+30 g / L sucrose + 7 g / L carrageenan); in step ((3), after 15 days of culture, the survival rate of the explants was 100%, the leaves were obviously enlarged, the leaf margins were upturned, and the induction rate of callus cells (or tissues) 100%. After cultivating for 35 days, the leaves of the explants transformed into massive bud clusters, the differentiation rate was 100%, the height of the bud clusters was about 0-7 mm, the growth rate of the bud clusters was faster, and a small amount of complete leaves were formed. Meanwhile, the bud clusters The inside of the clump tissue emits white fluff, and then the fluff grows rapidly into fine roots densely covered with white fluff, and the root tissue is relatively rich. After 45 days of cultivation, the seedling clump begins to form, with bulbs appearing at the base, and the lea...

Embodiment 3

[0038] Other operations are the same as in Example 2, except that in step (1), the formula of the medium is (MS+0.25 mg / L 6-BA+0.8 mg / L NAA+0.2 mg / L IBA+30 g / L sucrose + 7 g / L carrageenan); in step ((3), after 15 days of culture, the survival rate of the explants was 100%, the leaves were significantly enlarged and arched, the leaf margins were upturned, and the callus induction rate was 100%. After 35 days of culture, the leaves of the explants had rapidly transformed into massive bud clusters, the differentiation rate was 100%, the height of the bud clusters was about 2-7 mm, the bud clusters grew vigorously, and a large number of complete leaves were formed. At the same time, the base of the bud clusters had A large number of white fluffy fine roots are formed, and the root system is rich. After 40 days of cultivation, the seedling clusters begin to form, with obvious bulbs at the base, and the leaves are stretched and green. After 65 days of cultivation, the seedling clust...

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Abstract

The invention relates to a one-step seedling and efficient in-vitro propagation method for Begoniaceae gynura bicolor, which is an endangered plant with homology of medicine and food. The method provided by the invention comprises the following steps of: preparing a culture medium, and adjusting the culture medium through preparing, subpackaging and sterilizing; carrying out sterile inoculating directly on gynura bicolor aseptic seedling leaves which are taken as explants; culturing the inoculated culture medium in a culture chamber for 70-80 days under appropriate conditions, wherein the number of a single explant induced seedlings is 20 above; and decapping, transplanting regenerating plants to appropriate conditions, and culturing without hardening-seedling, wherein the commodity seedling survival rate achieves 100%. According to the method provided by the invention, the gynura bicolor tissue culture seedling regeneration period is short; the original seed property is kept effectively; the vegetative propagation coefficient is improved obviously; the operation is simple and convenient; the culture program is simple; the seedling cost is lowered obviously; and the method can act as an effective technology of industrialized production of high-quality seedlings of Begoniaceae gynura bicolor.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a tissue culture method for rapid seedling propagation of rare and endangered plants, in particular to a one-step seedling rapid propagation method for leaf tissue culture of the medicinal and edible homologous plant Begoniaceae Gelonia chinensis. Background technique [0002] Purple-backed geranium ( Begonia fimbristipulata Hance) is a succulent herb belonging to the family Begoniaceae Begonia, a species endemic to China, also known as Danye, Red Heliotrope, and Sanxuezi. It is commonly found in valleys, cliffs, and mosses under sparse forests at an altitude of 200-1 120 m. On wet rocks, it likes warm, humid and cool climates, and is generally distributed in clusters or clusters. The associated plants include mosses, small ferns, and shrubs. Glycyrrhizae can be found in Shenzhen, Dongguan, Zhaoqing and Jiaoling in Guangdong, but Dinghushan in Zhaoqing is the most famous, and its ty...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 邵玲梁廉梁广坚
Owner ZHAOQING UNIV
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