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Method for establishing efficient regeneration system by taking dandelion roots as explants

A high-efficiency regeneration and dandelion technology, applied in the biological field, can solve the problems of low regeneration frequency and lack of system, and achieve the effect of maintaining the characteristics of the original species, simplifying the seedling breeding procedures, and improving the asexual reproduction coefficient.

Inactive Publication Date: 2016-01-06
NINGXIA ACADEMY OF AGRI & FORESTRY SCI
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Problems solved by technology

[0003] In order to conduct more in-depth research on genetic transformation and active ingredients of dandelion, a rapid and efficient regeneration system is the basic experimental platform needed to establish and carry out these works, and the existing work is not systematic, and the regeneration frequency is low

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  • Method for establishing efficient regeneration system by taking dandelion roots as explants

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Embodiment Construction

[0022] The specific embodiments of the present invention will be further described below in conjunction with the accompanying drawings.

[0023] The operation of the inventive method comprises:

[0024] (1) Induction of sterile dandelion seedlings in test tubes: take healthy and rooted sterile dandelion seedlings, cut off the root segments, blot the water with sterilized filter paper, then cut the root segments into root segments, and inoculate the root segments into in MS medium. The concentration of NAA in MS medium is 0-2.0mg / L, the concentration of 6-BA is 0-1.0mg / L, the concentration of sucrose and agar is 30-40g / L and 4-7g / L respectively. When cultured for 20-30 days, the incidence rate of clustered buds induced by each explant reached 98.8%, and an average of 18-36 seedlings emerged from a single explant. The tissue culture seedlings grew well and basically maintained the characteristics of the original species.

[0025] (2) Cultivation of clustered shoots of test-tub...

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Abstract

The invention relates to a method for establishing an efficient regeneration system by taking medical and edible plant dandelion roots as explants. The method comprises the following steps: (1) putting the dandelion roots in an MS medium to induce one-step culture; (2) putting robust cluster buds or plants into a 1 / 2MS rooting medium, and performing cultivation to obtain complete seedlings with roots; (3) exercising the complete seedlings with the roots, putting the complete seedlings with the roots into a substrate to grow for 20 to 30d, and then transplanting the complete seedlings with the roots to a large field. The seedlings obtained by using the method are robust and high in survival rate, and a great number of high-quality seedlings of dandelion can be provided within a short time.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for establishing a high-efficiency regeneration system using dandelion root segments as explants. Background technique [0002] Dandelion (Taraxacum) is a perennial herb of the genus Taraxacum in the Compositae family. It belongs to about 2000 species and is mainly produced in the temperate to subtropical regions of the northern hemisphere. There are 70 species and 1 variant in China, which are widely distributed in Northeast, North, Northwest, Central and Southwest provinces. Dandelion is a traditional Chinese medicinal material, which has the effects of clearing away heat and detoxification, reducing swelling and dispelling stagnation, and diuresis for treating stranguria. Its chemical composition is complex, and the main active ingredients have antioxidant effects (flavonoids and antioxidant enzymes, etc.), antibacterial effects (chlorogenic acid) and anti-tu...

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Application Information

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IPC IPC(8): A01H4/00
Inventor 朱金霞张源沛郑国保孔德杰朱永兴吕学莲白海波
Owner NINGXIA ACADEMY OF AGRI & FORESTRY SCI
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