Method for establishing efficient regeneration system by taking dandelion roots as explants

A high-efficiency regeneration and dandelion technology, applied in the biological field, can solve the problems of low regeneration frequency and lack of system, and achieve the effect of maintaining the characteristics of the original species, simplifying the seedling breeding procedures, and improving the asexual reproduction coefficient.

Inactive Publication Date: 2016-01-06
NINGXIA ACADEMY OF AGRI & FORESTRY SCI
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[0003] In order to conduct more in-depth research on genetic transformation and active ingredients of dandelion, a rapid and efficient regeneration system is

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  • Method for establishing efficient regeneration system by taking dandelion roots as explants

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[0022] The specific embodiments of the present invention will be further described below with reference to the accompanying drawings.

[0023] The steps of the method of the present invention include:

[0024] ⑴Induction of dandelion sterile test-tube seedlings: Take sterile dandelion seedlings with healthy roots, cut off the roots, absorb water with sterilized filter paper, then cut the roots into roots, and inoculate the roots to MS medium. The concentration of NAA in MS medium is 0~2.0mg / L, the concentration of 6-BA is 0~1.0mg / L, the concentration of sucrose and agar are 30~40g / L and 4~7g / L, respectively. When cultured for 20-30 days, the incidence of cluster buds induced by each explant reached 98.8%, and a single explant formed 18-36 seedlings on average. The tissue culture seedlings grew well and basically maintained the characteristics of the original species.

[0025] ⑵ Rapid proliferation and cultivation of clump buds of test-tube seedlings: The robust plants obtained in s...

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Abstract

The invention relates to a method for establishing an efficient regeneration system by taking medical and edible plant dandelion roots as explants. The method comprises the following steps: (1) putting the dandelion roots in an MS medium to induce one-step culture; (2) putting robust cluster buds or plants into a 1/2MS rooting medium, and performing cultivation to obtain complete seedlings with roots; (3) exercising the complete seedlings with the roots, putting the complete seedlings with the roots into a substrate to grow for 20 to 30d, and then transplanting the complete seedlings with the roots to a large field. The seedlings obtained by using the method are robust and high in survival rate, and a great number of high-quality seedlings of dandelion can be provided within a short time.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for establishing a high-efficiency regeneration system using dandelion root segments as explants. Background technique [0002] Dandelion (Taraxacum) is a perennial herb of the genus Taraxacum in the Compositae family. It belongs to about 2000 species and is mainly produced in the temperate to subtropical regions of the northern hemisphere. There are 70 species and 1 variant in China, which are widely distributed in Northeast, North, Northwest, Central and Southwest provinces. Dandelion is a traditional Chinese medicinal material, which has the effects of clearing away heat and detoxification, reducing swelling and dispelling stagnation, and diuresis for treating stranguria. Its chemical composition is complex, and the main active ingredients have antioxidant effects (flavonoids and antioxidant enzymes, etc.), antibacterial effects (chlorogenic acid) and anti-tu...

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Application Information

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IPC IPC(8): A01H4/00
Inventor 朱金霞张源沛郑国保孔德杰朱永兴吕学莲白海波
Owner NINGXIA ACADEMY OF AGRI & FORESTRY SCI
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