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The invention discloses preparation and application of a porcine interleukin 17, 22 and 29 co-expression biological agent. The invention provides a fusion protein. The fusion protein comprises a porcine interleukin 17, a porcine interleukin 22 and a porcine interleukin 29. The recombinant yeast Po1h-IL17 / 22 / 29 capable of expressing porcine IL-17, IL-22 and IL-29 fusion molecules is constructed, and cells and mice are treated by the recombinant yeast Po1h-IL17 / 22 / 29, so that the cells and the mice obtain higher immune resistance to virus and bacterial infection. Subsequently, the piglets are orally inoculated with Po1h-IL17 / 22 / 29, and it is proved that the Po1h-IL17 / 22 / 29 obviously improves the growth performance and the whole body immunity of the piglets.