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Pickering emulsion, preparation method thereof and application of pickering emulsion as vaccine immunologic adjuvant

An emulsion and vaccine technology, applied in the field of immunology, can solve the problems of unclear dosage, immunization procedure and degree of antigen saving, unknown immune response mechanism, and difficulty in measuring the auxiliary effect of emulsion adjuvant.

Active Publication Date: 2021-09-10
NANHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004]1. Security and stability need to be further improved;
[0005]2. The mechanism of immune enhancement has not been elucidated. Current studies believe that emulsion adjuvants are usually related to immune cell recruitment and antigen uptake, and tend to Th1-type immune responses. TLR is irrelevant, but the underlying immune response mechanism is unknown;
[0006]3. The specific dose, immunization procedure and degree of antigen saving are not clear, and it is difficult to measure the extent to which the emulsion adjuvant can assist the vaccine. Approved adjuvants compared to whether they work better

Method used

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  • Pickering emulsion, preparation method thereof and application of pickering emulsion as vaccine immunologic adjuvant
  • Pickering emulsion, preparation method thereof and application of pickering emulsion as vaccine immunologic adjuvant
  • Pickering emulsion, preparation method thereof and application of pickering emulsion as vaccine immunologic adjuvant

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Embodiment 1

[0039] Embodiment 1 Preparation of GO-LP pickering emulsion of the present invention

[0040] Graphene oxide (sheet diameter is 0.5~5 μm) and water are mixed, and obtaining graphene oxide concentration is the aqueous phase of 1mg / mL;

[0041] Using liquid paraffin as the oil phase, mix the water phase and the oil phase at a ratio of 10:2, sonicate at a power of 325w for 10s with an interval of 10s, and sonicate repeatedly for a total of 10min to obtain GO-LP pickering emulsion. Morphological observation results see figure 1 -b, Microscopic observation results see figure 2 -b, (400×), the particle size and uniformity test results of the Malvern particle size analyzer are shown in image 3 -b, the embedding rate of BSA by GO-LP Pickering was determined to be 80%.

Embodiment 2

[0042] Embodiment 2 Preparation of GO-LP pickering emulsion of the present invention

[0043] Graphene oxide (sheet diameter is 0.5~5 μm) and water are mixed, and obtaining graphene oxide concentration is the aqueous phase of 1mg / mL;

[0044] Using liquid paraffin as the oil phase, mix the water phase and the oil phase at a ratio of 10:1, sonicate at 325w for 10s with an interval of 10s, and repeat the sonication for a total of 10min to obtain GO-LP pickering emulsion. Morphological observation results see figure 1 -a, Microscopic observation results see figure 2 -a, Malvern particle size analyzer to detect particle size and uniformity test results see image 3 -a.

Embodiment 3

[0045] Embodiment 3 Preparation of GO-LP pickering emulsion of the present invention

[0046] Graphene oxide (sheet diameter is 0.5~5 μm) and water are mixed, and obtaining graphene oxide concentration is the aqueous phase of 1mg / mL;

[0047] Using liquid paraffin as the oil phase, mix the water phase and the oil phase at a ratio of 10:4, sonicate at 325w for 10s with an interval of 10s, and repeat the sonication for a total of 10min to obtain GO-LP pickering emulsion.

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Abstract

The invention relates to the technical field of immunology, in particular to a pickering emulsion, a preparation method thereof and application of the pickering emulsion as a vaccine immunologic adjuvant. The GO-LP Pickering emulsion prepared by the preparation method disclosed by the invention shows good stability and an obviously-enhanced adjuvant effect through a stability test and in-vivo and in-vitro evaluation; a mouse genital tract anti-Ct infection model is established by taking a CtpORF5 recombinant protein vaccine as a model antigen, the immune protection capability and the safety of the GO-LPPickering adjuvant for the organism are evaluated, and a result shows that the GO-LPPickering emulsion provided by the invention can remarkably enhance the humoral immunity of a mouse and is relatively high in safety. Therefore, the pickering emulsion has potential values of enhancing immune response and improving the immune protection of the vaccine.

Description

technical field [0001] The invention relates to the technical field of immunology, in particular to a pickering emulsion, a preparation method thereof and an application as a vaccine immune adjuvant. Background technique [0002] Infectious diseases have always been the biggest threat to human health, especially the diseases caused by new pathogens often bring huge challenges to global health, seriously affecting human production and life, and even fatal blows. Adjuvants can reduce the dosage of vaccines, enhance immunogenicity, and effectively deal with vaccine production and supply problems during outbreaks. Adjuvants currently approved for use mainly include aluminum salts, emulsions, and Toll-like receptor agonists. Although traditional aluminum adjuvants have been widely used in many vaccines, they can only enhance humoral immunity but not cellular immunity and have limited effect on subunit vaccines. Other adjuvants such as liposomes, immune regulators, oligonucleotid...

Claims

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Application Information

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IPC IPC(8): A61K39/39A61K39/118A61K9/107A61P37/04A61P31/04
CPCA61K39/39A61K39/118A61K9/1075A61P37/04A61P31/04A61K2039/55505A61K2039/575A61K2300/00Y02A50/30
Inventor 李忠玉赵兰华舒明艺陈虹亮
Owner NANHUA UNIV
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