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Porcine circovivus2 strain and application thereof

A porcine circovirus and strain technology, applied in antiviral agents, virus/bacteriophage, virus antigen components, etc., can solve problems such as inability to accurately measure virus titer, unsatisfactory vaccine preventive effect, rough vaccine preparation process, etc., to achieve Prevent the occurrence or prevalence of circovirus disease, shorten the immune gap, and improve the immune effect

Active Publication Date: 2015-01-07
YEBIO BIOENG OF QINGDAO
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In this regard, PCV2 vaccine research has been carried out at home and abroad, some of which have been approved and produced, and have played a certain role in immune prevention, but the disease still occurs in various places, and the pathogen can be detected in healthy pigs, indicating that The preventive effect of the existing PCV2 vaccine is not ideal. The reason is that in addition to breeding and disease factors, it may also be related to the low antigen content of the vaccine (the production cell line is PK15, PCV2 cannot cause it to produce CPE, and the virus efficacy cannot be accurately measured. price), rough vaccine preparation process, strain mutation and other factors

Method used

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  • Porcine circovivus2 strain and application thereof
  • Porcine circovivus2 strain and application thereof
  • Porcine circovivus2 strain and application thereof

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Embodiment Construction

[0010] The present invention will be described in detail below in conjunction with the examples.

[0011] 1. Screening of PCV2SD strain of porcine circovirus type 2 (Porcine Circovirus 2) strain

[0012] 1. Screening of virus strains

[0013] A virus strain was isolated from the lymph nodes, spleen, lungs and other tissues of piglets with suspected PMWS in a farm in Shandong Province in 2005. The fresh tissue samples of diseased pigs were fully ground, treated with double antibodies (penicillin and streptomycin), centrifuged at 12000rpm / min at 4°C for 10min, filtered through a 0.22μm microporous membrane, and tested for PCV2, porcine pseudorabies virus (PRV ), porcine reproductive and respiratory syndrome virus (PRRSV), swine fever virus (CSFV), porcine parvovirus (PPV) and other PCR / RT-PCR tests, it was confirmed that PCV2 was purely positive, and the supernatant of the treated samples was inoculated with MPR cells After isolation and cultivation, a highly proliferative PCV...

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Abstract

The invention provides a porcine circovivus2 strain and application thereof. The novel porcine circovivus2 strain is good in productivity and good in immunogenicity. A vaccine prepared through inactivation of the porcine circovivus2 strain can effectively prevent the porcine circovivus disease. The preservation serial number of the porcine circovivus2 strain is CGMCC NO. 7707. According to the porcine circovivus2 strain (PCV2SD), protective efficacy is good, MPR cells used for strain reproduction can be reproduced well with conventional methods, inoculation can be achieved synchronously as well as with conventional methods, and the virus titer obtained after inoculated culture can reach more than 105.25 TCID / 0.1ml; the vaccine prepared with the PCV2SD can well prevent the occurrence or prevalence of the porcine circovivus disease, and the protection rate can reach more than 90% if ELISA detection is carried out after immunity. Compared with other vaccines of the same kind in the domestic market in respect of protective efficacy, the vaccine prepared with the PCV2SD has the advantages of being fast in antibody production, long in protecting period, and capable of shortening the immunity blank area obviously, reducing immunity frequency, and improving economic performance.

Description

technical field [0001] The invention belongs to the technical field of livestock and poultry pathogenic bacteria screening, and in particular relates to a porcine circovirus type 2 strain and application thereof. Background technique [0002] In 1991, John Harding of Canada reported Postweaning Multisystemic Wasting Syndrome (PMWS), and after extensive and in-depth investigation and research, it was confirmed in 1998 that porcine circovirus (Porcine Circovirus, PCV) was the main pathogen. It has been confirmed that porcine circovirus disease (Porcine Circovirus Disease, PCVD) is a clinical symptom caused by porcine circovirus type 2 (Porcine Circovirus2, PCV2) infection, which is progressive weight loss, dyspnea, shortness of breath, anemia, Viral diseases of diarrhea, jaundice, interstitial pneumonia, lymphadenitis and nephritis, mainly affecting weaned piglets aged 5 to 12 weeks, and PMWS, porcine dermatitis and nephritis syndrome (Porcine Dermatitis and Nephropathy Syndro...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N7/00A61K39/12A61P31/20C12R1/93
Inventor 邹敏张恒刘新文申洪银宫晓刘蕾邹桂荣韩乃君陶晓珊
Owner YEBIO BIOENG OF QINGDAO
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