Porcine circovivus2 strain and application thereof
A porcine circovirus and strain technology, applied in antiviral agents, virus/bacteriophage, virus antigen components, etc., can solve problems such as inability to accurately measure virus titer, unsatisfactory vaccine preventive effect, rough vaccine preparation process, etc., to achieve Prevent the occurrence or prevalence of circovirus disease, shorten the immune gap, and improve the immune effect
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[0010] The present invention will be described in detail below in conjunction with the examples.
[0011] 1. Screening of PCV2SD strain of porcine circovirus type 2 (Porcine Circovirus 2) strain
[0012] 1. Screening of virus strains
[0013] A virus strain was isolated from the lymph nodes, spleen, lungs and other tissues of piglets with suspected PMWS in a farm in Shandong Province in 2005. The fresh tissue samples of diseased pigs were fully ground, treated with double antibodies (penicillin and streptomycin), centrifuged at 12000rpm / min at 4°C for 10min, filtered through a 0.22μm microporous membrane, and tested for PCV2, porcine pseudorabies virus (PRV ), porcine reproductive and respiratory syndrome virus (PRRSV), swine fever virus (CSFV), porcine parvovirus (PPV) and other PCR / RT-PCR tests, it was confirmed that PCV2 was purely positive, and the supernatant of the treated samples was inoculated with MPR cells After isolation and cultivation, a highly proliferative PCV...
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