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Application of nocardia immunopotentiator in fish vaccine

An immune enhancer, the technology of Nocardia, which is applied in the field of medicine and biology, can solve the problems of no obvious promotion of immune effect, insignificant promotion effect, poor immune effect, etc., and achieves high safety, wide application range and immunity. strong effect

Pending Publication Date: 2021-11-16
广东渔跃生物技术有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this promotion is aimed at specific macrophages, and the scope of application is narrow, and the range of strains that can be selected is also narrow, and there is no obvious promotion effect on the immune effect of animals
[0006] In summary, the immunopotentiators in the prior art generally have the disadvantages of poor immune effect, narrow scope of application, and inconspicuous promoting effect.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Embodiment 1 A kind of preparation process of immune adjuvant

[0032] A nocardia immune enhancer, the immune enhancer is composed of the following components: 28% nocardia, 30% pachyphyllin and 42% hyaluronic acid.

[0033] The preparation method of Nocardia immunopotentiator comprises the steps:

[0034] 1), Nocardia was placed in a 30L fermenter and cultivated for 48 hours, inactivated by high temperature and high pressure to obtain the inactivated thallus of Nocardia; described step 1) the formula of the medium in the fermenter was glucose 2% , yeast powder 2%, tryptone 3%, purified water 95%, pH7.0; the culture conditions in the fermentor are: stirring speed 310r / min, ventilation rate 1:10, inoculum size 8%, liquid volume It is 65% of the tank volume, and the temperature is 30°C; the specific operation of the high temperature and high pressure inactivation is 120°C, 101kPa, and 22min.

[0035] 2), take each raw material by weight percentage proportioning;

[003...

Embodiment 2

[0038] Embodiment 2 A kind of preparation process of immune adjuvant

[0039] A nocardia immune enhancer, the immune enhancer is composed of the following components: 30% nocardia, 25% pachyphyllin and 45% hyaluronic acid.

[0040] The preparation method of Nocardia immunopotentiator comprises the steps:

[0041]1), Nocardia was placed in a 30L fermenter and cultivated for 50 hours, inactivated by high temperature and high pressure to obtain the inactivated thallus of Nocardia; described step 1) the formula of the medium in the fermenter was glucose 3% , yeast powder 1%, tryptone 5%, purified water 96%, pH7.1; the culture conditions in the fermenter are: stirring speed 310r / min, ventilation rate 1:10, inoculum size 7%, liquid volume It is 65% of the tank volume, and the temperature is 30°C; the specific operation of the high temperature and high pressure inactivation is 122°C, 101kPa, 20min.

[0042] 2), take each raw material by weight percentage proportioning;

[0043] 3)...

Embodiment 3

[0045] Embodiment 3 A kind of preparation process of immune adjuvant

[0046] A Nocardia immune enhancer, the immune enhancer is composed of the following components: 28% Nocardia, 17% pachyphyllin and 55% hyaluronic acid.

[0047] The preparation method of Nocardia immunopotentiator comprises the steps:

[0048] 1), Nocardia was placed in a 30L fermenter and cultivated for 46 hours, inactivated by high temperature and high pressure to obtain the inactivated thallus of Nocardia; described step 1) the formula of the medium in the fermenter was glucose 4% , yeast powder 2%, tryptone 3%, purified water 93%, pH 7.2; the culture conditions in the fermentor are: stirring speed 315r / min, ventilation rate 1:10, inoculum size 8%, liquid volume It is 65% of the tank volume, and the temperature is 30°C; the specific operation of the high temperature and high pressure inactivation is 121°C, 101kPa, 20min.

[0049] 2), take each raw material by weight percentage proportioning;

[0050] ...

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PUM

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Abstract

The invention provides an application of a nocardia immunopotentiator in a fish vaccine, the nocardia immunopotentiator comprises nocardia, pachymaran and hyaluronic acid, and the invention also provides an application of the nocardia immunopotentiator in an oil emulsion inactivated vaccine for fish. According to the fish oil emulsion inactivated vaccine, vaccines aiming at different viruses or bacteria can be obtained according to different selected antigens; animal experiments prove that the nocardia immunopotentiator and the application thereof in fish vaccines enable the fish vaccines to be strong in immunity, and the oil emulsion vaccine added with the nocardia adjuvant has the advantages of high safety, no obvious residue in local vaccine injection, simple preparation process and wide application range, Tand the corresponding vaccine specificity is high.

Description

technical field [0001] The invention belongs to the technical field of medicine and biology, and in particular relates to the application of a Nocardia immune enhancer in fish vaccines. Background technique [0002] Immunopotentiators refer to substances that can enhance the body’s immune response either alone or simultaneously with antigens. There are many varieties of adjuvants, which can be divided into three categories according to their prerequisites: (1) Immune substitutes, used to replace certain Drugs with biological factors that enhance immunity; (2) immune recovery agents, which can enhance the suppressed immune function, but have little effect on normal immune function; (3) immune adjuvants, also known as non-specific stimulators. Commonly used immune enhancers include BCG, Corynebacterium pumilus, endotoxin, immune ribonucleic acid, thymosin, transfer factor, double-stranded polynucleotide, aluminum hydroxide adjuvant and so on. [0003] Although some of the abo...

Claims

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Application Information

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IPC IPC(8): A61K39/39
CPCA61K39/39A61K2039/55594A61K2039/55583A61K2039/55A61K2039/54
Inventor 张毓金王文勇严悌昆黄淑芬
Owner 广东渔跃生物技术有限公司
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