Application of nocardia immunopotentiator in fish vaccine
An immune enhancer, the technology of Nocardia, which is applied in the field of medicine and biology, can solve the problems of no obvious promotion of immune effect, insignificant promotion effect, poor immune effect, etc., and achieves high safety, wide application range and immunity. strong effect
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Examples
Example Embodiment
[0031] Example 1 Preparation process of immunoassine
[0032] A Norcocci immunological reinforcing agent, which consists of 2% Norcocci, 30% 苓 polysaccharide and 42% hyaluronic acid.
[0033] The preparation method of Norcocci immunochagegen, including the following steps:
[0034] 1), cultured Norca Steel in 30 L of the fermenter for 48 hours, high temperature and high pressure inactivation, and obtain Norca bacteria sterilization; the step 1) The formulation of the medium in the fermentor is 2% glucose 2% 2% of yeast powder, 3% of trypsin, purified water 95%, pH 7.0; the culture conditions in the fermenter are: stirring speed 310R / min, ventilation 1:10, inoculation amount 8%, loading liquid It is 65% of the tank, temperature 30 ° C; the high temperature and high pressure inactivation is 120 ° C, 101 kPa, and the treatment 22min.
[0035] 2), each raw material is weighed according to the weight of the weight;
[0036] 3), the Norca bacteria, the polysaccharide and hyaluronic ac...
Example Embodiment
[0038] Embodiment 2 Preparation process of an immunoassine
[0039] A Norcocci immunological reinforcing agent, which consists of 30% Norcocci, 25% 苓 polysaccharide and 45% hyaluronic acid.
[0040] The preparation method of Norcocci immunochagegen, including the following steps:
[0041]1), cultured in 30 L of the fermenter in 30 L, high temperature and high pressure inactivation, and obtaining Norca bacteria to sinking the live bacteria; the formulation of the medium in the fermentor is 3% glucose 3% 1% of yeast powder, 5% of trypsin, purified water 96%, pH 7.1; the culture conditions in the fermenter are: stirring rotational speed 310R / min, ventilation 1:10, inoculation amount of 7%, liquid content It is 65% of the tank to accumulate at a temperature of 30 ° C; the specific operation of the high temperature and high pressure inactivation is 122 ° C, 101 kPa, and treat 20 min.
[0042] 2), each raw material is weighed according to the weight of the weight;
[0043] 3), the Nor...
Example Embodiment
[0045] Example 3 Preparation process of immunoassine
[0046] A Norcocci immunological reinforcing agent, which consists of the following components: 28% Norcocci, 17% 苓 polysaccharide and 55% hyaluronic acid.
[0047] The preparation method of Norcocci immunochagegen, including the following steps:
[0048] 1), the Norca bacteria were placed in a fermentation tank of 30L for 46 hours, high temperature and high pressure, and obtained Norca bacteria, the formulation of the cells described in the first fermentor was 4% of glucose 4%. 2% of yeast powder, 3% of trypsin, purified water 93%, pH 7.2; the culture conditions in the fermenter are: stirring speed 315R / min, ventilation 1:10, inoculation amount 8%, inoculation It is 65% of the tank to accumulate, temperature 30 ° C; the specific operation of the high temperature and high pressure inactivation is 121 ° C, 101 kPa, and treat 20 min.
[0049] 2), each raw material is weighed according to the weight of the weight;
[0050] 3), t...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap