The invention relates to a formula donkey milk formula with an anti-tumor efficacy. The formula donkey milk powder is characterized in that donkey milk powder is taken as the main material, and components, including lactobacillus acidophilus, lactobacillus rhamnosus, lactobacillus plantarum, lentinan, pachymaran, grifolan, fructo-oligosaccharide, galactooligosaccharide, isomaltose hypgather, synanthrin, polysaccharides in reed rhizome, apricot kernel meal, walnut powder, Chinese yam powder, lotus seed powder, lily root flour, wolfberry powder and the like are added. The formula donkey milk powder has the effects of inhibiting the growth of tumor cells and prolonging the living time of tumor-bearing mice.